WTAP Antibody - #DF3282

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Product: WTAP Antibody
Catalog: DF3282
Description: Rabbit polyclonal antibody to WTAP
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 44 KD; 44kD(Calculated).
Uniprot: Q15007
RRID: AB_2835650

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(90%), Sheep(100%), Dog(90%), Chicken(90%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
WTAP Antibody detects endogenous levels of total WTAP.
RRID:
AB_2835650
Cite Format: Affinity Biosciences Cat# DF3282, RRID:AB_2835650.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp686F20131; Female-lethal(2)D homolog; FL2D_HUMAN; hFL(2)D; KIAA0105; MGC3925; Mum2; OTTHUMP00000017522; OTTHUMP00000017523; PNAS 132; PNAS-132; PNAS132; Pre mRNA splicing regulator WTAP; Pre-mRNA-splicing regulator WTAP; Putative pre mRNA splicing regulator female lethal 2D homolog; Putative pre mRNA splicing regulator female lethal(2D); Putative pre-mRNA splicing regulator female lethal(2D) homolog; putative pre-mRNA splicing regulator female-lethal(2D); Wilms tumor 1 associated protein; Wilms tumor 1 associating protein; Wilms tumor 1-associating protein; Wilms' tumor 1 associating protein; Wilms' tumour 1-associating protein; WT1 associated protein; WT1-associated protein; WT1-associating protein; wtap;

Immunogens

Immunogen:
Uniprot:

Q15007(FL2D_HUMAN) >>Visit HPA database.

Gene(ID):
WTAP(9589)
Expression:
Q15007 FL2D_HUMAN:

Ubiquitously expressed.

Sequence:
MTNEEPLPKKVRLSETDFKVMARDELILRWKQYEAYVQALEGKYTDLNSNDVTGLRESEEKLKQQQQESARRENILVMRLATKEQEMQECTTQIQYLKQVQQPSVAQLRSTMVDPAINLFFLKMKGELEQTKDKLEQAQNELSAWKFTPDSQTGKKLMAKCRMLIQENQELGRQLSQGRIAQLEAELALQKKYSEELKSSQDELNDFIIQLDEEVEGMQSTILVLQQQLKETRQQLAQYQQQQSQASAPSTSRTTASEPVEQSEATSKDCSRLTNGPSNGSSSRQRTSGSGFHREGNTTEDDFPSSPGNGNKSSNSSEERTGRGGSGYVNQLSAGYESVDSPTGSENSLTHQSNDTDSSHDPQEEKAVSGKGNRTVGSRHVQNGLDSSVNVQGSVL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Sheep
100
Horse
90
Dog
90
Xenopus
90
Chicken
90
Zebrafish
78
Pig
0
Bovine
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15007 As Substrate

Site PTM Type Enzyme
M1 Acetylation
T2 Phosphorylation
K10 Ubiquitination
S14 Phosphorylation
K19 Ubiquitination
K31 Ubiquitination
K43 Ubiquitination
Y44 Phosphorylation
S49 Phosphorylation
S58 Phosphorylation
K61 Ubiquitination
K63 Ubiquitination
S69 Phosphorylation
K83 Ubiquitination
K98 Ubiquitination
K125 Sumoylation
K125 Ubiquitination
K134 Ubiquitination
K146 Ubiquitination
T148 Phosphorylation
K191 Ubiquitination
S244 Phosphorylation
S267 Phosphorylation
C270 S-Nitrosylation
T274 Phosphorylation
S288 Phosphorylation
S290 Phosphorylation
T298 Phosphorylation
T299 Phosphorylation
S305 Phosphorylation
S306 Phosphorylation
S313 Phosphorylation
S314 Phosphorylation
S316 Phosphorylation
S317 Phosphorylation
S341 Phosphorylation
T343 Phosphorylation
S345 Phosphorylation
S348 Phosphorylation
S388 Phosphorylation

Research Backgrounds

Function:

Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing. Required for accumulation of METTL3 and METTL14 to nuclear speckle. Acts as a mRNA splicing regulator. Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes.

Subcellular Location:

Nucleus speckle. Nucleus>Nucleoplasm. Cytoplasm.
Note: Mainly nuclear with some fraction located in the cytoplasm. ZC3H13 is required to anchor component of the MACOM subcomplex, such as VIRMA, in the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Component of the WMM complex, a N6-methyltransferase complex composed of a catalytic subcomplex, named MAC, and of an associated subcomplex, named MACOM. The MAC subcomplex is composed of METTL3 and METTL14. The MACOM subcomplex is composed of WTAP, ZC3H13, CBLL1/HAKAI, VIRMA, and, in some cases of RBM15 (RBM15 or RBM15B). Interacts with WT1. Also component of a MACOM-like complex, named WTAP complex, composed of WTAP, ZC3H13, CBLL1, VIRMA, RBM15, BCLAF1 and THRAP3.

Family&Domains:

Belongs to the fl(2)d family.

References

1). Comprehensive Analysis of the Transcriptome-Wide m6A Methylation Modification Difference in Liver Fibrosis Mice by High-Throughput m6A Sequencing. Frontiers in Cell and Developmental Biology, 2021 (PubMed: 34869362) [IF=5.5]

Application: WB    Species: Mice    Sample: Liver Fibrosis

FIGURE 7 Verification of m6A methylation level and methylase expression in LF. (A) The m6A methylation level in LF. (B) Semiquantitative analysis of m6A methylation. (C) IGV plots of the WTAP m6A level. (D) IGV plots of the ALKBH5 m6A level. (E) IGV plots of the YTHDF1 m6A level. (F) IGV plots of the WTAP expression level. (G) IGV plots of the ALKBH5 expression level. (H) IGV plots of the YTHDF1 expression level. (I) mRNA expression level of WTAP. (J) mRNA expression level of ALKBH5. (K) mRNA expression level of YTHDF1. (L) Protein expression levels of WTAP. (M) Protein expression levels of ALKBH5. (N) Protein expression levels of YTHDF1. (O) Semiquantitative analysis of WTAP protein. (P) Semiquantitative analysis of YTHDF1 protein. (Q) Semiquantitative analysis of ALKBH5 protein. ## p < 0.01 compared with the control group, # p < 0.05 compared with the control group.

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