ZNF147 Antibody - #DF3276

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Product: ZNF147 Antibody
Catalog: DF3276
Description: Rabbit polyclonal antibody to ZNF147
Application: WB IHC IF/ICC
Reactivity: Human, Monkey
Prediction: Pig, Bovine, Rabbit, Dog, Xenopus
Mol.Wt.: 71 KD; 71kD(Calculated).
Uniprot: Q14258
RRID: AB_2835644

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Monkey
Prediction:
Pig(92%), Bovine(100%), Rabbit(92%), Dog(92%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
ZNF147 Antibody detects endogenous levels of total ZNF147.
RRID:
AB_2835644
Cite Format: Affinity Biosciences Cat# DF3276, RRID:AB_2835644.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

E3 ubiquitin/ISG15 ligase TRIM25; EFP; Estrogen responsive finger protein; Estrogen-responsive finger protein; RING finger protein 147; RNF 147; RNF147; TRI25; TRI25_HUMAN; TRIM 25; Trim25; Tripartite motif containing 25; Tripartite motif containing protein 25; Tripartite motif protein TRIM25; Tripartite motif-containing protein 25; Tripartite motifcontaining25; Ubiquitin/ISG15-conjugating enzyme TRIM25; Z147; Zinc finger protein 147 (estrogen responsive finger protein); Zinc finger protein 147; Zinc finger protein-147; ZNF 147; ZNF147;

Immunogens

Immunogen:
Uniprot:

Q14258(TRI25_HUMAN) >>Visit HPA database.

Gene(ID):
TRIM25(7706)
Expression:
Q14258 TRI25_HUMAN:

Expressed in breast tumors (at protein level). Ubiquitous.

Sequence:
MAELCPLAEELSCSICLEPFKEPVTTPCGHNFCGSCLNETWAVQGSPYLCPQCRAVYQARPQLHKNTVLCNVVEQFLQADLAREPPADVWTPPARASAPSPNAQVACDHCLKEAAVKTCLVCMASFCQEHLQPHFDSPAFQDHPLQPPVRDLLRRKCSQHNRLREFFCPEHSECICHICLVEHKTCSPASLSQASADLEATLRHKLTVMYSQINGASRALDDVRNRQQDVRMTANRKVEQLQQEYTEMKALLDASETTSTRKIKEEEKRVNSKFDTIYQILLKKKSEIQTLKEEIEQSLTKRDEFEFLEKASKLRGISTKPVYIPEVELNHKLIKGIHQSTIDLKNELKQCIGRLQEPTPSSGDPGEHDPASTHKSTRPVKKVSKEEKKSKKPPPVPALPSKLPTFGAPEQLVDLKQAGLEAAAKATSSHPNSTSLKAKVLETFLAKSRPELLEYYIKVILDYNTAHNKVALSECYTVASVAEMPQNYRPHPQRFTYCSQVLGLHCYKKGIHYWEVELQKNNFCGVGICYGSMNRQGPESRLGRNSASWCVEWFNTKISAWHNNVEKTLPSTKATRVGVLLNCDHGFVIFFAVADKVHLMYKFRVDFTEALYPAFWVFSAGATLSICSPK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Pig
92
Dog
92
Rabbit
92
Xenopus
80
Chicken
67
Horse
0
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14258 As Substrate

Site PTM Type Enzyme
S46 Phosphorylation
Y48 Phosphorylation
Y57 Phosphorylation
T67 Phosphorylation
T91 Phosphorylation
S97 Phosphorylation
S100 Phosphorylation
K112 Ubiquitination
K117 Ubiquitination
S187 Phosphorylation
S190 Phosphorylation
S192 Phosphorylation
K205 Ubiquitination
K237 Ubiquitination
Y245 Phosphorylation
T246 Phosphorylation
K249 Ubiquitination
T258 Phosphorylation
K268 Ubiquitination
K273 Acetylation
K273 Ubiquitination
T276 Phosphorylation
Y278 Phosphorylation
K283 Methylation
K283 Ubiquitination
K284 Methylation
K284 Ubiquitination
K285 Ubiquitination
T290 Phosphorylation
K292 Ubiquitination
K301 Ubiquitination
K310 Ubiquitination
S312 Phosphorylation
T319 Phosphorylation
K320 Acetylation
K320 Ubiquitination
Y323 Phosphorylation
K332 Ubiquitination
K335 Ubiquitination
S340 Phosphorylation
K345 Ubiquitination
K349 Ubiquitination
K392 Ubiquitination
K402 Acetylation
K402 Ubiquitination
T405 Phosphorylation
K416 Methylation
K416 Ubiquitination
K425 Methylation
K425 Ubiquitination
T427 Phosphorylation
S429 Phosphorylation
S433 Phosphorylation
T434 Phosphorylation
S435 Phosphorylation
K437 Ubiquitination
K439 Ubiquitination
K447 Ubiquitination
Y455 Phosphorylation
K508 Ubiquitination
K509 Ubiquitination
K567 Acetylation
K567 Ubiquitination
K573 Ubiquitination

Research Backgrounds

Function:

Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58 and IFIH1. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region and may play a role in signal transduction that leads to the production of interferons in response to viral infection. Mediates 'Lys-63'-linked polyubiquitination of IFIH1. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3.

PTMs:

Auto-ISGylated.

Subcellular Location:

Cytoplasm. Cytoplasm>Stress granule.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in breast tumors (at protein level). Ubiquitous.

Subunit Structure:

Interacts (via SPRY domain) with DDX58 (via CARD domain). Interacts with ZFHX3. Interacts with NLRP12; this interaction reduces the E3 ubiquitin ligase TRIM25-mediated 'Lys-63'-linked DDX58 activation.

(Microbial infection) Interacts (via coiled coil) with influenza A virus NS1 protein; this interaction specifically inhibits TRIM25 multimerization and TRIM25-mediated DDX58 CARD ubiquitination, thereby suppressing DDX58 signal transduction.

Family&Domains:

The RING-type zinc finger is important for ISG15 E3 ligase activity and autoISGylation. AutoISGylation negatively regulates ISG15 E3 ligase activity.

The C-terminal B30.2/SPRY domain interacts with the first N-terminal CARD domain of DDX58.

Research Fields

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.   (View pathway)

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