GIT2 Antibody - #DF3274

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Product: GIT2 Antibody
Catalog: DF3274
Description: Rabbit polyclonal antibody to GIT2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 84 KD; 85kD(Calculated).
Uniprot: Q14161
RRID: AB_2835642

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
GIT2 Antibody detects endogenous levels of total GIT2.
RRID:
AB_2835642
Cite Format: Affinity Biosciences Cat# DF3274, RRID:AB_2835642.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ARF GAP GIT2; ARF GTPase activating protein GIT2; ARF GTPase-activating protein GIT2; CAT 2; CAT-2; CAT2; Cool associated tyrosine phosphorylated protein 2; Cool interacting tyrosine phosphorylated protein 2; Cool-interacting tyrosine-phosphorylated protein 2; DKFZp686G01261; G protein coupled receptor kinase interacting ArfGAP 2; G protein coupled receptor kinase interactor 2; G protein-coupled receptor kinase-interactor 2; GIT 2; GIT2; GIT2_HUMAN; GRK interacting protein 2; GRK-interacting protein 2; KIAA0148; MGC760;

Immunogens

Immunogen:
Uniprot:

Q14161(GIT2_HUMAN) >>Visit HPA database.

Gene(ID):
GIT2(9815)
Sequence:
MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKVNNNLSDELRIMQKKLQTLQSENSNLRKQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPMGEASRPEESRMRLQPFPAHIGRSALVTSSSSLPSFPSTLSWSRDESARRASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPGSPTDVQLVTQQVIQCAYDIAKAAKQLVTITTKENNN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
91
Xenopus
91
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14161 As Substrate

Site PTM Type Enzyme
S7 Phosphorylation
S8 Phosphorylation
S36 Phosphorylation
K98 Ubiquitination
K103 Ubiquitination
S127 Phosphorylation
K131 Ubiquitination
K135 Ubiquitination
S154 Phosphorylation
T195 Phosphorylation Q13315 (ATM)
Y286 Phosphorylation
S361 Phosphorylation
S365 Phosphorylation
K366 Ubiquitination
T375 Phosphorylation
S381 Phosphorylation
S384 Phosphorylation Q13315 (ATM)
Y392 Phosphorylation
S394 Phosphorylation
S397 Phosphorylation
T401 Phosphorylation
T405 Phosphorylation
T406 Phosphorylation
S408 Phosphorylation
S415 Phosphorylation
S418 Phosphorylation
S421 Phosphorylation
T426 Phosphorylation
K434 Ubiquitination
K443 Ubiquitination
K449 Ubiquitination
K464 Ubiquitination
K477 Ubiquitination
T480 Phosphorylation
T481 Phosphorylation
Y484 Phosphorylation
T488 Phosphorylation
S490 Phosphorylation
Y492 Phosphorylation
T493 Phosphorylation
T495 Phosphorylation
S496 Phosphorylation
S500 Phosphorylation
S506 Phosphorylation
S510 Phosphorylation
S514 Phosphorylation
Y516 Phosphorylation
T518 Phosphorylation
S520 Phosphorylation
Y525 Phosphorylation
S532 Phosphorylation
S557 Phosphorylation
S558 Phosphorylation
S559 Phosphorylation
S562 Phosphorylation
S565 Phosphorylation
S568 Phosphorylation
S570 Phosphorylation
S586 Phosphorylation
T587 Phosphorylation
S590 Phosphorylation
Y592 Phosphorylation
S614 Phosphorylation
S668 Phosphorylation
S673 Phosphorylation
S693 Phosphorylation
T698 Phosphorylation
S699 Phosphorylation
S724 Phosphorylation
K747 Ubiquitination

Research Backgrounds

Function:

GTPase-activating protein for the ADP ribosylation factor family.

Subunit Structure:

Interacts with TGFB1I1 (By similarity). Interacts with G protein-coupled receptor kinases. Associates with paxillin. Also interacts with PIX exchange factors. Identified in a complex with ARHGEF6 and BIN2.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

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For Research Use Only.
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