CBF beta Antibody - #DF3273
| Product: | CBF beta Antibody |
| Catalog: | DF3273 |
| Description: | Rabbit polyclonal antibody to CBF beta |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Dog, Xenopus |
| Mol.Wt.: | 22 KD; 22kD(Calculated). |
| Uniprot: | Q13951 |
| RRID: | AB_2835641 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF3273, RRID:AB_2835641.
Fold/Unfold
CBF b; CBF beta; CBF-beta; CBFB; CBFbeta; Core binding factor beta subunit; core binding factor subunit beta; Core-binding factor subunit beta; PEA 2; PEA2; PEA2 beta; PEA2-beta; PEA2beta; PEBB_HUMAN; PEBP 2B; PEBP2 beta; PEBP2-beta; PEBP2B; PEBP2beta; Polyomavirus enhancer binding protein 2 beta subunit; Polyomavirus enhancer-binding protein 2 beta subunit; SL3 3 enhancer factor 1 beta subunit; SL3 3 enhancer factor 1 subunit beta; SL3-3 enhancer factor 1 subunit beta; SL3/AKV core binding factor beta subunit; SL3/AKV core-binding factor beta subunit;
Immunogens
- Q13951 PEBB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQARFQNACRDGRSEIAFVATGTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLQRLDGMGCLEFDEERAQQEDALAQQAFEEARRRTREFEDRDRSHREEMEVRVSQLLAVTGKKTTRP
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13951 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K11 | Acetylation | Uniprot | |
| K11 | Ubiquitination | Uniprot | |
| R19 | Methylation | Uniprot | |
| C25 | S-Nitrosylation | Uniprot | |
| K28 | Acetylation | Uniprot | |
| K28 | Ubiquitination | Uniprot | |
| T80 | Phosphorylation | Uniprot | |
| Y85 | Phosphorylation | Uniprot | |
| K94 | Ubiquitination | Uniprot | |
| Y96 | Phosphorylation | Uniprot | |
| T150 | Phosphorylation | Uniprot | |
| S169 | Phosphorylation | Uniprot | |
| K177 | Acetylation | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| K178 | Ubiquitination | Uniprot |
Research Backgrounds
Forms the heterodimeric complex core-binding factor (CBF) with RUNX family proteins (RUNX1, RUNX2, and RUNX3). RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation.
Nucleus.
Heterodimer with RUNX1, RUNX2 and RUNX3. Interacts with COPRS. Found in a complex with PRMT5 and RUNX1.
Belongs to the CBF-beta family.
Restrictive clause
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For Research Use Only.
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