TNAP1 Antibody - #DF3271

(3)  
Product: TNAP1 Antibody
Catalog: DF3271
Description: Rabbit polyclonal antibody to TNAP1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 34 KD; 36kD(Calculated).
Uniprot: Q13829
RRID: AB_2835639

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(90%), Bovine(90%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(80%)
Clonality:
Polyclonal
Specificity:
TNAP1 Antibody detects endogenous levels of total TNAP1.
RRID:
AB_2835639
Cite Format: Affinity Biosciences Cat# DF3271, RRID:AB_2835639.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

alpha-induced protein 1; BACD2_HUMAN; BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2; BTB/POZ domain-containing protein TNFAIP1; endothelial; hBACURD2; Protein B12; tnfaip1; Tumor necrosis factor alpha induced protein 1 (endothelial); Tumor necrosis factor alpha induced protein 1; Tumor necrosis factor;

Immunogens

Immunogen:
Uniprot:

Q13829(BACD2_HUMAN) >>Visit HPA database.

Gene(ID):
TNFAIP1(7126)
Sequence:
MSGDTCLCPASGAKPKLSGFKGGGLGNKYVQLNVGGSLYYTTVRALTRHDTMLKAMFSGRMEVLTDKEGWILIDRCGKHFGTILNYLRDDTITLPQNRQEIKELMAEAKYYLIQGLVNMCQSALQDKKDSYQPVCNIPIITSLKEEERLIESSTKPVVKLLYNRSNNKYSYTSNSDDHLLKNIELFDKLSLRFNGRVLFIKDVIGDEICCWSFYGQGRKLAEVCCTSIVYATEKKQTKVEFPEARIYEETLNVLLYETPRVPDNSLLEATSRSRSQASPSEDEETFELRDRVRRIHVKRYSTYDDRQLGHQSTHRD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Sheep
100
Dog
100
Rabbit
100
Pig
90
Bovine
90
Chicken
80
Xenopus
50
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13829 As Substrate

Site PTM Type Enzyme
S11 Phosphorylation
K14 Ubiquitination
K16 Ubiquitination
K21 Ubiquitination
K67 Ubiquitination
K78 Ubiquitination
K102 Ubiquitination
S142 Phosphorylation
K144 Ubiquitination
K155 Ubiquitination
K159 Ubiquitination
Y162 Phosphorylation
K168 Ubiquitination
K181 Ubiquitination
K188 Ubiquitination
T237 Phosphorylation
K238 Ubiquitination
Y256 Phosphorylation
S265 Phosphorylation
S278 Phosphorylation P68400 (CSNK2A1)
S280 Phosphorylation P68400 (CSNK2A1)
T285 Phosphorylation

Research Backgrounds

Function:

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(TNFAIP1) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. Its interaction with RHOB may regulate apoptosis. May enhance the PCNA-dependent DNA polymerase delta activity.

PTMs:

Phosphorylation at Ser-280 by CK2 facilitates the nucleus localization and increases interaction with PCNA.

Subcellular Location:

Cytoplasm. Nucleus. Endosome.
Note: Colocalizes with RHOB in endosomes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the BCR(TNFAIP1) E3 ubiquitin ligase complex, at least composed of CUL3, TNFAIP1/BACURD2 and RBX1. Interacts with RHOA; with a preference for RhoA-GDP. Interacts with RHOB. Interacts with PCNA. Interacts with CSNK2B.

Family&Domains:

Belongs to the BACURD family.

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