CAF1A Antibody - #DF3254

(3)  
Product: CAF1A Antibody
Catalog: DF3254
Description: Rabbit polyclonal antibody to CAF1A
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep, Dog
Mol.Wt.: 150 KD; 107kD(Calculated).
Uniprot: Q13111
RRID: AB_2835634

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
CAF1A Antibody detects endogenous levels of total CAF1A.
RRID:
AB_2835634
Cite Format: Affinity Biosciences Cat# DF3254, RRID:AB_2835634.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CAF 1; CAF 1 subunit A; CAF; CAF I 150 kDa subunit; CAF I p150; CAF-1 subunit A; CAF-I 150 kDa subunit; CAF-I p150; CAF1; CAF1A_HUMAN; CAF1B; CAF1P150; CHAF1A; Chromatin assembly factor 1 subunit A; Chromatin Assembly Factor 1 Subunit A p150; Chromatin assembly factor I (150 kDa); Chromatin assembly factor I 150 kDa; Chromatin assembly factor I p150 subunit; DCAF1; hp150; MGC71229; P150;

Immunogens

Immunogen:
Uniprot:

Q13111(CAF1A_HUMAN) >>Visit HPA database.

Gene(ID):
CHAF1A(10036)
Sequence:
MLEELECGAPGARGAATAMDCKDRPAFPVKKLIQARLPFKRLNLVPKGKADDMSDDQGTSVQSKSPDLEASLDTLENNCHVGSDIDFRPKLVNGKGPLDNFLRNRIETSIGQSTVIIDLTEDSNEQPDSLVDHNKLNSEASPSREAINGQREDTGDQQGLLKAIQNDKLAFPGETLSDIPCKTEEEGVGCGGAGRRGDSQECSPRSCPELTSGPRMCPRKEQDSWSEAGGILFKGKVPMVVLQDILAVRPPQIKSLPATPQGKNMTPESEVLESFPEEDSVLSHSSLSSPSSTSSPEGPPAPPKQHSSTSPFPTSTPLRRITKKFVKGSTEKNKLRLQRDQERLGKQLKLRAEREEKEKLKEEAKRAKEEAKKKKEEEKELKEKERREKREKDEKEKAEKQRLKEERRKERQEALEAKLEEKRKKEEEKRLREEEKRIKAEKAEITRFFQKPKTPQAPKTLAGSCGKFAPFEIKEHMVLAPRRRTAFHPDLCSQLDQLLQQQSGEFSFLKDLKGRQPLRSGPTHVSTRNADIFNSDVVIVERGKGDGVPERRKFGRMKLLQFCENHRPAYWGTWNKKTALIRARDPWAQDTKLLDYEVDSDEEWEEEEPGESLSHSEGDDDDDMGEDEDEDDGFFVPHGYLSEDEGVTEECADPENHKVRQKLKAKEWDEFLAKGKRFRVLQPVKIGCVWAADRDCAGDDLKVLQQFAACFLETLPAQEEQTPKASKRERRDEQILAQLLPLLHGNVNGSKVIIREFQEHCRRGLLSNHTGSPRSPSTTYLHTPTPSEDAAIPSKSRLKRLISENSVYEKRPDFRMCWYVHPQVLQSFQQEHLPVPCQWSYVTSVPSAPKEDSGSVPSTGPSQGTPISLKRKSAGSMCITQFMKKRRHDGQIGAEDMDGFQADTEEEEEEEGDCMIVDVPDAAEVQAPCGAASGAGGGVGVDTGKATLTASPLGAS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Pig
67
Horse
55
Zebrafish
50
Xenopus
45
Chicken
45
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13111 As Substrate

Site PTM Type Enzyme
K22 Acetylation
K30 Acetylation
S54 Phosphorylation
S65 Phosphorylation
S71 Phosphorylation
T74 Phosphorylation
S83 Phosphorylation
T114 Phosphorylation
S123 Phosphorylation
S138 Phosphorylation
S141 Phosphorylation
S143 Phosphorylation
T154 Phosphorylation
K162 Ubiquitination
K168 Ubiquitination
S177 Phosphorylation
K182 Sumoylation
S199 Phosphorylation
S203 Phosphorylation
S206 Phosphorylation
T211 Phosphorylation
S224 Phosphorylation
K234 Ubiquitination
S255 Phosphorylation
T259 Phosphorylation
T266 Phosphorylation
S308 Phosphorylation
T309 Phosphorylation
S310 Phosphorylation
T314 Phosphorylation
S315 Phosphorylation
T316 Phosphorylation
T330 Phosphorylation
T454 Phosphorylation
K467 Acetylation
K467 Ubiquitination
K513 Sumoylation
T527 Phosphorylation
S535 Phosphorylation
K544 Sumoylation
S642 Phosphorylation
K666 Ubiquitination
K674 Acetylation
K674 Ubiquitination
T722 Phosphorylation
S767 Phosphorylation
T770 Phosphorylation
S772 Phosphorylation
S775 Phosphorylation
S777 Phosphorylation
T778 Phosphorylation
T779 Phosphorylation
Y780 Phosphorylation
T783 Phosphorylation
T785 Phosphorylation
S794 Phosphorylation
K795 Sumoylation
S796 Phosphorylation
S803 Phosphorylation
S853 Phosphorylation
S858 Phosphorylation
S862 Phosphorylation
T865 Phosphorylation
S868 Phosphorylation
K870 Methylation
K870 Ubiquitination
K872 Methylation
S873 Phosphorylation
S876 Phosphorylation
S933 Phosphorylation
T947 Phosphorylation
T949 Phosphorylation
S951 Phosphorylation

Research Backgrounds

Function:

Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci (By similarity).

Subcellular Location:

Nucleus.
Note: DNA replication foci.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer. Part of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. CHAF1A binds directly to PCNA and to CBX1. Binds MBD1. Interacts directly with CBX5 via the PxVxL motif. During DNA replication, it forms a S phase-specific complex that facilitates DNA methylation and histone H3 'Lys-9' methylation during replication-coupled chromatin assembly and is at least composed of the CHAF1A, MBD1 and SETDB1. Interacts with CBX5.

Family&Domains:

Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.

Belongs to the CHAF1A family.

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