ELAVL2 Antibody - #DF3251

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Product: ELAVL2 Antibody
Catalog: DF3251
Description: Rabbit polyclonal antibody to ELAVL2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 38 KD; 40kD(Calculated).
Uniprot: Q12926
RRID: AB_2835631

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 100ul $280 In stock
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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(91%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
ELAVL2 Antibody detects endogenous levels of total ELAVL2.
RRID:
AB_2835631
Cite Format: Affinity Biosciences Cat# DF3251, RRID:AB_2835631.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ELAV like neuron specific RNA binding protein 2; ELAV like Neuronal Protein 1; ELAV like Protein 2; ELAV-like neuronal protein 1; ELAV-like protein 2; ELAV2_HUMAN; ELAVL2; HEL-N1; HELN1; HU Antigen B; Hu-antigen B; HuB; Nervous System Specific RNA Binding Protein MelN1; Nervous system-specific RNA-binding protein Hel-N1;

Immunogens

Immunogen:
Uniprot:

Q12926(ELAV2_HUMAN) >>Visit HPA database.

Gene(ID):
ELAVL2(1993)
Expression:
Q12926 ELAV2_HUMAN:

Brain; neural-specific.

Sequence:
METQLSNGPTCNNTANGPTTINNNCSSPVDSGNTEDSKTNLIVNYLPQNMTQEELKSLFGSIGEIESCKLVRDKITGQSLGYGFVNYIDPKDAEKAINTLNGLRLQTKTIKVSYARPSSASIRDANLYVSGLPKTMTQKELEQLFSQYGRIITSRILVDQVTGISRGVGFIRFDKRIEAEEAIKGLNGQKPPGATEPITVKFANNPSQKTNQAILSQLYQSPNRRYPGPLAQQAQRFRLDNLLNMAYGVKRFSPMTIDGMTSLAGINIPGHPGTGWCIFVYNLAPDADESILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYDEAAMAIASLNGYRLGDRVLQVSFKTNKTHKA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
91
Xenopus
82
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q12926 As Substrate

Site PTM Type Enzyme
T51 Phosphorylation
K74 Ubiquitination
K95 Ubiquitination
T99 Phosphorylation
K111 Ubiquitination
Y114 Phosphorylation
S118 Phosphorylation
S119 Phosphorylation
T162 Phosphorylation
S165 Phosphorylation
K209 Ubiquitination
S221 Phosphorylation
K315 Ubiquitination

Research Backgrounds

Function:

RNA-binding protein that binds to the 3' untranslated region (3'UTR) of target mRNAs (By similarity). Seems to recognize a GAAA motif (By similarity). Can bind to its own 3'UTR, the FOS 3'UTR and the ID 3'UTR (By similarity).

Tissue Specificity:

Brain; neural-specific.

Subunit Structure:

Interacts with IGF2BP1. Interacts with MAP1B light chain LC1 (By similarity).

Family&Domains:

Belongs to the RRM elav family.

References

1). Dihydroartemisinin Attenuates Hypoxia-Induced Pulmonary Hypertension Through the ELAVL2/miR-503/PI3K/AKT Axis. Journal of cardiovascular pharmacology, 2022 (PubMed: 35512032) [IF=2.6]

Application: WB    Species: Rat    Sample: PASMCs

FIGURE 6. ELAVL2 regulated PASMCs migration and proliferation negatively. (A), (B), Immunoblotting analysis of ELAVL2 expression in PASMCs transfected with an ELAVL2-overexpressing plasmid or a control plasmid (n =3). (C), (D), Cell migration was determined by transwell assays at 24 hours (n = 3) (×100). (E), (F), EdU incorporation experiments (n = 3) were used to evaluate cell viability (×200). (G), (H), Immunoblotting analysis of ELAVL2 and PCNA expression in PASMCs of each treatment group (n = 3). OE means overexpression of ELAVL2.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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