Nuclear Factor 1 Antibody - #DF3250

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Product: Nuclear Factor 1 Antibody
Catalog: DF3250
Description: Rabbit polyclonal antibody to Nuclear Factor 1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Rabbit, Dog, Chicken
Mol.Wt.: 55 KD; 56kD,47kD,55kD(Calculated).
Uniprot: Q12857 | O00712 | P08651 | Q14938
RRID: AB_2835630

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
Nuclear Factor 1 Antibody detects endogenous levels of total Nuclear Factor 1.
RRID:
AB_2835630
Cite Format: Affinity Biosciences Cat# DF3250, RRID:AB_2835630.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1110047K16Rik; 9430022M17Rik; CCAAT Box Binding Transcription Factor; CCAAT-box-binding transcription factor; CTF; FLJ39164; NF I/A; NF-I/A; NF1-A; NFI A; NFI L; NFI-A; NFIA; NFIA_HUMAN; Nuclear factor 1 A type; Nuclear factor 1 A-type; Nuclear factor 1/A; Nuclear factor I/A; TGGCA binding protein; TGGCA-binding protein; CCAAT Box Binding Transcription Factor; CCAAT-box-binding transcription factor; CTF; HMGIC/NFIB; NF-I/B; NF1-B; NF1B; NF1B2; NFI-B; NFI-RED; Nfib; NFIB_HUMAN; NFIB2; NFIB3; Nuclear factor 1 B-type; Nuclear factor 1/B; Nuclear Factor 1B; Nuclear Factor I B; Nuclear factor I/B; TGGCA Binding Protein; TGGCA-binding protein; TRANSCRIPTION FACTOR NFIB; 1110019L22Rik; 1500041O16Rik; AA589446; AI746521; CAAT box transcription factor; CCAAT binding transcription factor; CCAAT box binding transcription factor; CCAAT-box-binding transcription factor; CNFI C; CTF; CTF5; MGC137374; MGC20153; NF I; NF I/C; NF-I/C; NF1 C; NF1-C; NF1C; NFI; NFI-C; NFI/C; NFIC; NFIC_HUMAN; Nuclear factor 1; Nuclear factor 1 C type; Nuclear factor 1 C-type; Nuclear factor 1/C; Nuclear factor I/C (CCAAT binding transcription factor); Nuclear factor I/C; TGGCA binding protein; TGGCA-binding protein; Transcription factor NFIC; CCAAT box binding transcription factor; CCAAT-box-binding transcription factor; CTF; NF-I/X; NF1-X; NF1A; NF1X; NFI X; NFI-X; NFI/X; NFIX; NFIX_HUMAN; Nuclear factor 1 X type; Nuclear factor 1 X-type; Nuclear factor 1/X; Nuclear factor I/X; TGGCA binding protein; TGGCA-binding protein;

Immunogens

Immunogen:
Uniprot:

Q12857(NFIA_HUMAN) >>Visit HPA database.

O00712(NFIB_HUMAN) >>Visit HPA database.

P08651(NFIC_HUMAN) >>Visit HPA database.

Q14938(NFIX_HUMAN) >>Visit HPA database.

Gene(ID):
NFIA(4774) | NFIB(4781) | NFIC(4782) | NFIX(4784)
Expression:
Q14938 NFIX_HUMAN:

Widely expressed.

Sequence:
MYSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKEEERAVKDELLSEKPEVKQKWASRLLAKLRKDIRPEYREDFVLTVTGKKPPCCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKSPQCSNPGLCVQPHHIGVSVKELDLYLAYFVHAADSSQSESPSQPSDADIKDQPENGHLGFQDSFVTSGVFSVTELVRVSQTPIAAGTGPNFSLSDLESSSYYSMSPGAMRRSLPSTSSTSSTKRLKSVEDEMDSPGEEPFYTGQGRSPGSGSQSSGWHEVEPGMPSPTTLKKSEKSGFSSPSPSQTSSLGTAFTQHHRPVITGPRASPHATPSTLHFPTSPIIQQPGPYFSHPAIRYHPQETLKEFVQLVCPDAGQQAGQVGFLNPNGSSQGKVHNPFLPTPMLPPPPPPPMARPVPLPVPDTKPPTTSTEGGAASPTSPTYSTPSTSPANRFVSVGPRDPSFVNIPQQTQSWYLG

MMYSPICLTQDEFHPFIEALLPHVRAIAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLSEKPEIKQKWASRLLAKLRKDIRQEYREDFVLTVTGKKHPCCVLSNPDQKGKIRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLMKSPHCTNPALCVQPHHITVSVKELDLFLAYYVQEQDSGQSGSPSHNDPAKNPPGYLEDSFVKSGVFNVSELVRVSRTPITQGTGVNFPIGEIPSQPYYHDMNSGVNLQRSLSSPPSSKRPKTISIDENMEPSPTGDFYPSPSSPAAGSRTWHERDQDMSSPTTMKKPEKPLFSSASPQDSSPRLSTFPQHHHPGIPGVAHSVISTRTPPPPSPLPFPTQAILPPAPSSYFSHPTIRYPPHLNPQDTLKNYVPSYDPSSPQTSQSWYLG

MYSSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLGEKPEVKQKWASRLLAKLRKDIRPECREDFVLSITGKKAPGCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKAAQCGHPVLCVQPHHIGVAVKELDLYLAYFVRERDAEQSGSPRTGMGSDQEDSKPITLDTTDFQESFVTSGVFSVTELIQVSRTPVVTGTGPNFSLGELQGHLAYDLNPASTGLRRTLPSTSSSGSKRHKSGSMEEDVDTSPGGDYYTSPSSPTSSSRNWTEDMEGGISSPVKKTEMDKSPFNSPSPQDSPRLSSFTQHHRPVIAVHSGIARSPHPSSALHFPTTSILPQTASTYFPHTAIRYPPHLNPQDPLKDLVSLACDPASQQPGPLNGSGQLKMPSHCLSAQMLAPPPPGLPRLALPPATKPATTSEGGATSPTSPSYSPPDTSPANRSFVGLGPRDPAGIYQAQSWYLG

MYSPYCLTQDEFHPFIEALLPHVRAFSYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLGEKPEIKQKWASRLLAKLRKDIRPEFREDFVLTITGKKPPCCVLSNPDQKGKIRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLYKSPQCSNPGLCVQPHHIGVTIKELDLYLAYFVHTPESGQSDSSNQQGDADIKPLPNGHLSFQDCFVTSGVWNVTELVRVSQTPVATASGPNFSLADLESPSYYNINQVTLGRRSITSPPSTSTTKRPKSIDDSEMESPVDDVFYPGTGRSPAAGSSQSSGWPNDVDAGPASLKKSGKLDFCSALSSQGSSPRMAFTHHPLPVLAGVRPGSPRATASALHFPSTSIIQQSSPYFTHPTIRYHHHHGQDSLKEFVQFVCSDGSGQATGQPNGSGQGKVPGSFLLPPPPPVARPVPLPMPDSKSTSTAPDGAALTPPSPSFATTGASSANRFVSIGPRDGNFLNIPQQSQSWFL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q12857/O00712/P08651/Q14938 As Substrate

Site PTM Type Enzyme
S3 Phosphorylation
K64 Ubiquitination
T145 Phosphorylation
S232 Phosphorylation
T240 Phosphorylation
S247 Phosphorylation
S256 Phosphorylation
S258 Phosphorylation
S265 Phosphorylation
S268 Phosphorylation
S270 Phosphorylation
S271 Phosphorylation
T272 Phosphorylation
S273 Phosphorylation
S274 Phosphorylation
T275 Phosphorylation
K276 Acetylation
K276 Ubiquitination
S280 Phosphorylation
S287 Phosphorylation
Y294 Phosphorylation
S300 Phosphorylation
S303 Phosphorylation
S305 Phosphorylation
S307 Phosphorylation
S308 Phosphorylation
S319 Phosphorylation
T321 Phosphorylation
T322 Phosphorylation
S332 Phosphorylation
S333 Phosphorylation
S335 Phosphorylation
T344 Phosphorylation
S360 Phosphorylation
T364 Phosphorylation
T372 Phosphorylation
S373 Phosphorylation
R389 Methylation
S469 Phosphorylation
T471 Phosphorylation
S472 Phosphorylation
T474 Phosphorylation
Y475 Phosphorylation
T480 Phosphorylation
S495 Phosphorylation
Site PTM Type Enzyme
S4 Phosphorylation
S50 Phosphorylation
K58 Ubiquitination
S63 Phosphorylation
K65 Ubiquitination
K100 Ubiquitination
K112 Ubiquitination
T146 Phosphorylation
S194 Phosphorylation
S196 Phosphorylation
Y207 Phosphorylation
S211 Phosphorylation
K214 Ubiquitination
S221 Phosphorylation
S246 Phosphorylation
S262 Phosphorylation
S264 Phosphorylation
S265 Phosphorylation
S268 Phosphorylation
S269 Phosphorylation
S276 Phosphorylation
S284 Phosphorylation
T286 Phosphorylation
Y290 Phosphorylation
S292 Phosphorylation
S294 Phosphorylation
S295 Phosphorylation
S312 Phosphorylation
T314 Phosphorylation
S325 Phosphorylation
S326 Phosphorylation
S328 Phosphorylation
S332 Phosphorylation
S333 Phosphorylation
R335 Methylation
T359 Phosphorylation
S364 Phosphorylation
R388 Methylation
K400 Methylation
S410 Phosphorylation
T413 Phosphorylation
Site PTM Type Enzyme
M1 Acetylation
S4 Phosphorylation
K58 Ubiquitination
K65 Ubiquitination
K99 Ubiquitination
K112 Ubiquitination
T146 Phosphorylation
S192 Phosphorylation
S194 Phosphorylation
T237 Phosphorylation
T270 Phosphorylation
S273 Phosphorylation
S275 Phosphorylation
S276 Phosphorylation
S277 Phosphorylation
S279 Phosphorylation
K280 Acetylation
S284 Phosphorylation
S286 Phosphorylation
T293 Phosphorylation
S294 Phosphorylation
Y299 Phosphorylation
T301 Phosphorylation
S302 Phosphorylation
S304 Phosphorylation
S305 Phosphorylation
T307 Phosphorylation
S310 Phosphorylation
T314 Phosphorylation
S322 Phosphorylation
S323 Phosphorylation
T328 Phosphorylation
S333 Phosphorylation
S337 Phosphorylation
S339 Phosphorylation
S343 Phosphorylation
R345 Methylation
T350 Phosphorylation
S361 Phosphorylation
R365 Methylation
S366 Phosphorylation
S371 Phosphorylation
R395 Methylation
S427 Phosphorylation
S438 Phosphorylation
R451 Methylation
T472 Phosphorylation
S473 Phosphorylation
S475 Phosphorylation
Y476 Phosphorylation
S477 Phosphorylation
T481 Phosphorylation
S482 Phosphorylation
S487 Phosphorylation
Site PTM Type Enzyme
S3 Phosphorylation
K57 Ubiquitination
K64 Ubiquitination
T145 Phosphorylation
T233 Phosphorylation
S244 Phosphorylation
S250 Phosphorylation
Y253 Phosphorylation
S265 Phosphorylation
T267 Phosphorylation
S268 Phosphorylation
S271 Phosphorylation
T272 Phosphorylation
S273 Phosphorylation
T274 Phosphorylation
S280 Phosphorylation
S284 Phosphorylation
S288 Phosphorylation
S301 Phosphorylation
S306 Phosphorylation
S307 Phosphorylation
S309 Phosphorylation
S322 Phosphorylation
K324 Ubiquitination
S336 Phosphorylation
S340 Phosphorylation
S341 Phosphorylation
S361 Phosphorylation
S381 Phosphorylation
Y383 Phosphorylation
T388 Phosphorylation
S399 Phosphorylation
T463 Phosphorylation
S466 Phosphorylation

Research Backgrounds

Function:

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Binds DNA as a homodimer.

Family&Domains:

The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Belongs to the CTF/NF-I family.

Function:

Transcriptional activator of GFAP, essential for proper brain development. Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Binds DNA as a homodimer.

Family&Domains:

The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Belongs to the CTF/NF-I family.

Function:

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Binds DNA as a homodimer.

Family&Domains:

The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Belongs to the CTF/NF-I family.

Function:

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Binds DNA as a homodimer.

Family&Domains:

The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Belongs to the CTF/NF-I family.

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