PCBP2 Recombinant Rabbit mAb - #BF4466

(1)   Copy Info
Product: PCBP2 Recombinant Rabbit mAb
Catalog: BF4466
Description: Rabbit monoclonal antibody to PCBP2
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 35-45 kDa(Observed); 39kD(Calculated).
Uniprot: Q15366

View similar products>>

Out of stock
Control Products
Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit IgG
Application:
WB 1:1000, ICC
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human, Mouse, Rat
Clonality:
Monoclonal [ReFirm23916]
Specificity:
PCBP2 Recombinant Rabbit mAb detects endogenous levels of PCBP2.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Alpha CP2; Alpha-CP2; alphaCP-2; Cbp; CTBP; Heterogeneous nuclear ribonucleoprotein E2; Heterogenous nuclear ribonucleoprotein E2; hnRNP E2; hnRNP-E2; HNRNPE2; Hnrnpx; HNRPE2; Hnrpx; MGC110998; PCBP2; PCBP2_HUMAN; poly(rC) binding protein 2; Poly(rC)-binding protein 2; Putative heterogeneous nuclear ribonucleoprotein X; rCbinding protein 2;

Immunogens

Immunogen:

A synthetic peptide from human PCBP2

Uniprot:

Q15366(PCBP2_HUMAN) >>Visit HPA database.

Gene(ID):
PCBP2(5094)
Expression:
Q15366 PCBP2_HUMAN:

Detected in all tissues examined.

Description:
The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK,it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1,this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES,promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA,human papillomavirus type 16 L2 mRNA,and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1,an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. Thsi gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene.
Sequence:
MDTGVIEGGLNVTLTIRLLMHGKEVGSIIGKKGESVKKMREESGARINISEGNCPERIITLAGPTNAIFKAFAMIIDKLEEDISSSMTNSTAASRPPVTLRLVVPASQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGIPQSIIECVKQICVVMLETLSQSPPKGVTIPYRPKPSSSPVIFAGGQDRYSTGSDSASFPHTTPSMCLNPDLEGPPLEAYTIQGQYAIPQPDLTKLHQLAMQQSHFPMTHGNTGFSGIESSSPEVKGYWGLDASAQTTSHELTIPNDLIGCIIGRQGAKINEIRQMSGAQIKIANPVEGSTDRQVTITGSAASISLAQYLINVRLSSETGGMGSS

Research Backgrounds

Function:

Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitination and degradation.

(Microbial infection) In case of infection by poliovirus, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation. Also plays a role in initiation of viral RNA replication in concert with the viral protein 3CD.

PTMs:

Phosphorylated. The non-phosphorylated form(s) exhibited the strongest poly(rC)-binding activity.

(Microbial infection) Proteolyticaly cleaved by picornavirus proteinase 3CD.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in all tissues examined.

Family&Domains:

The KH domains mediates poly(C) binding.

Research Fields

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.