MtSSB Antibody | Affinity Biosciences

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Western blot analysis of extracts from MCF7, using MtSSB Antibody.

Product:	MtSSB Antibody
Catalog:	DF3240
Description:	Rabbit polyclonal antibody to MtSSB
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	17 KD; 17kD(Calculated).
Uniprot:	Q04837
RRID:	AB_2835620

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

MtSSB Antibody detects endogenous levels of total MtSSB.

RRID:

AB_2835620
Cite Format: Affinity Biosciences Cat# DF3240, RRID:AB_2835620.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

mitochondrial; Mt SSB; Mt-SSB; MtSSB; PWP1 interacting protein 17; PWP1-interacting protein 17; SENSOR OF SINGLE-STRANDED DNA COMPLEX; Single stranded DNA binding protein 1; Single stranded DNA binding protein; Single stranded DNA binding protein mitochondrial; Single-stranded DNA-binding protein; SOSS B1; SOSS COMPLEX, SUBUNIT B1; SSBP 1; SSBP; SSBP_HUMAN; SSBP1;

Immunogens

Immunogen:

Uniprot:

Q04837(SSBP_HUMAN) >>Visit HPA database.

Gene(ID):

SSBP1(6742)

Sequence:

Q04837 SSBP_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MFRRPVLQVLRQFVRHESETTTSLVLERSLNRVHLLGRVGQDPVLRQVEGKNPVTIFSLATNEMWRSGDSEVYQLGDVSQKTTWHRISVFRPGLRDVAYQYVKKGSRIYLEGKIDYGEYMDKNNVRRQATTIIADNIIFLSDQTKEKE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Rabbit

100

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q04837 As Substrate

Q04837

Site	PTM Type	Enzyme	Source
K51	Acetylation		Uniprot
K51	Ubiquitination		Uniprot
S67	Phosphorylation	O14757 (CHEK1)	Uniprot
S70	Phosphorylation		Uniprot
Y73	Phosphorylation		Uniprot
S79	Phosphorylation		Uniprot
K81	Ubiquitination		Uniprot
S88	Phosphorylation		Uniprot
Y99	Phosphorylation		Uniprot
Y101	Phosphorylation		Uniprot
K103	Acetylation		Uniprot
Y109	Phosphorylation		Uniprot
K113	Acetylation		Uniprot
Y119	Phosphorylation		Uniprot
K122	Ubiquitination		Uniprot
T130	Phosphorylation		Uniprot
K145	Ubiquitination		Uniprot

Research Backgrounds

Q04837_SSBP_HUMAN

Function:

Binds preferentially and cooperatively to pyrimidine rich single-stranded DNA (ss-DNA). In vitro, required to maintain the copy number of mitochondrial DNA (mtDNA) and plays crucial roles during mtDNA replication that stimulate activity of the replisome components POLG and TWNK at the replication fork. Promotes the activity of the gamma complex polymerase POLG, largely by organizing the template DNA and eliminating secondary structures to favor ss-DNA conformations that facilitate POLG activity. In addition it is able to promote the 5'-3' unwinding activity of the mtDNA helicase TWNK. May also function in mtDNA repair.

Subcellular Location:

Mitochondrion. Mitochondrion matrix>Mitochondrion nucleoid.

Subunit Structure:

Homotetramer. Interacts with MPG/AAG, through inhibition of its glycosylase activity it potentially prevents formation of DNA breaks in ssDNA, ensuring that base removal primarily occurs in dsDNA. Interacts with POLDIP2. Interacts with PRIMPOL.

Research Fields

· Genetic Information Processing > Replication and repair > DNA replication.

· Genetic Information Processing > Replication and repair > Mismatch repair.

· Genetic Information Processing > Replication and repair > Homologous recombination.

Restrictive clause

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For Research Use Only.
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MtSSB Antibody - #DF3240