MEF2B Antibody | Affinity Biosciences

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Western blot analysis of extracts from HT-29 cells, using MEF2B antibody.

Product:	MEF2B Antibody
Catalog:	DF3236
Description:	Rabbit polyclonal antibody to MEF2B
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.:	38 KD; 39kD(Calculated).
Uniprot:	Q02080
RRID:	AB_2835616

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(82%), Bovine(82%), Horse(82%), Sheep(82%), Dog(91%)

Clonality:

Polyclonal

Specificity:

MEF2B Antibody detects endogenous levels of total MEF2B.

RRID:

AB_2835616
Cite Format: Affinity Biosciences Cat# DF3236, RRID:AB_2835616.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

MADS box transcription enhancer factor 2; MDS1 EVI1; Mef2b; MEF2B_HUMAN; Myocyte enhancer factor 2B; Myocyte specific enhancer factor 2B; Myocyte-specific enhancer factor 2B; PRDM3; RSRFR2; Serum response factor like protein 2; Serum response factor-like protein 2; XMEF2;

Immunogens

Immunogen:

Uniprot:

Q02080(MEF2B_HUMAN) >>Visit HPA database.

Gene(ID):

MEF2B(100271849)

Expression:

Q02080 MEF2B_HUMAN:

Expressed in skeletal and cardiac muscle and brain.

Sequence:

Q02080 MEF2B_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGRKKIQISRILDQRNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFNSANRLFQYASTDMDRVLLKYTEYSEPHESRTNTDILETLKRRGIGLDGPELEPDEGPEEPGEKFRRLAGEGGDPALPRPRLYPAAPAMPSPDVVYGALPPPGCDPSGLGEALPAQSRPSPFRPAAPKAGPPGLVHPLFSPSHLTSKTPPPLYLPTEGRRSDLPGGLAGPRGGLNTSRSLYSGLQNPCSTATPGPPLGSFPFLPGGPPVGAEAWARRVPQPAAPPRRPPQSASSLSASLRPPGAPATFLRPSPIPCSSPGPWQSLCGLGPPCAGCPWPTAGPGRRSPGGTSPERSPGTARARGDPTSLQASSEKTQQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

Pig

Horse

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q02080 As Substrate

Q02080

Site	PTM Type	Source
K23	Ubiquitination	Uniprot
K89	Ubiquitination	Uniprot
K112	Ubiquitination	Uniprot
T196	Phosphorylation	Uniprot

Research Backgrounds

Q02080_MEF2B_HUMAN

Function:

Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Activates transcription via this element. May be involved in muscle-specific and/or growth factor-related transcription.

Subcellular Location:

Nucleus.

Tissue Specificity:

Expressed in skeletal and cardiac muscle and brain.

Subunit Structure:

Interacts with HDAC7 (By similarity). Heterodimer. Interacts with HDAC9.

Family&Domains:

Belongs to the MEF2 family.

Research Fields

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

MEF2B Antibody - #DF3236