CKS1 Antibody - #DF3221

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Product: CKS1 Antibody
Catalog: DF3221
Description: Rabbit polyclonal antibody to CKS1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.: 9 KD; 10kD(Calculated).
Uniprot: P61024
RRID: AB_2835601

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
CKS1 Antibody detects endogenous levels of total CKS1.
RRID:
AB_2835601
Cite Format: Affinity Biosciences Cat# DF3221, RRID:AB_2835601.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CDC2 associated protein CKS1; CDC28 protein kinase 1; CDC28 protein kinase 1B; CDC28 protein kinase regulatory subunit 1B; Cell division control protein CKS1; CKS 1; CKS-1; CKS1; CKS1_HUMAN; Cks1b; Ckshs1; Cyclin dependent kinases regulatory subunit 1; Cyclin-dependent kinases regulatory subunit 1; NB4 apoptosis/differentiation related protein; PNAS 143; PNAS 16; PNAS 18;

Immunogens

Immunogen:
Uniprot:

P61024(CKS1_HUMAN) >>Visit HPA database.

Gene(ID):
CKS1B(1163)
Sequence:
MSHKQIYYSDKYDDEEFEYRHVMLPKDIAKLVPKTHLMSESEWRNLGVQQSQGWVHYMIHEPEPHILLFRRPLPKKPKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
91
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P61024 As Substrate

Site PTM Type Enzyme
S2 Acetylation
K4 Ubiquitination
Y7 Phosphorylation
Y8 Phosphorylation
S9 Phosphorylation
K11 Ubiquitination
Y12 Phosphorylation
Y19 Phosphorylation
K26 Ubiquitination
K30 Ubiquitination
K34 Ubiquitination
S39 Phosphorylation
S41 Phosphorylation
S51 Phosphorylation
Y57 Phosphorylation

Research Backgrounds

Function:

Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function.

Subunit Structure:

Forms a homohexamer that can probably bind six kinase subunits.

Family&Domains:

Belongs to the CKS family.

Research Fields

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

References

1). Identification of CKS1B as a prognostic indicator and a predictive marker for immunotherapy in pancreatic cancer. Frontiers in Immunology, 2022 (PubMed: 36405738) [IF=7.3]

Application: WB    Species: Human    Sample: PC cells

Figure 8 Knocking down CKS1B inhibits autophagy and STAT3/PD-L1 signaling in PC cells. (A, B) mRNA and protein expression of CKS1B in cells transfected with shCKS1B. (C) mRNA expression of PD-L1 was measured by qRT-PCR. (D) Protein levels of LC3B were measured by Western blotting. (E) Protein levels of p-STAT3 and PD-L1 were measured by western blotting.

Restrictive clause

 

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