ADK Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from various samples, using ADK Antibody.

Product:	ADK Antibody
Catalog:	DF3218
Description:	Rabbit polyclonal antibody to ADK
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	40 KD; 41kD(Calculated).
Uniprot:	P55263
RRID:	AB_2835598

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(92%), Dog(100%), Chicken(92%), Xenopus(83%)

Clonality:

Polyclonal

Specificity:

ADK Antibody detects endogenous levels of total ADK.

RRID:

AB_2835598
Cite Format: Affinity Biosciences Cat# DF3218, RRID:AB_2835598.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

2310026J05Rik; 5033405D03Rik; Adenosine 5''-phosphotransferase; Adenosine kinase; adk; ADK_HUMAN; AI255373; AI987814; AK; MGC6593; OTTHUMP00000019864; OTTHUMP00000019865;

Immunogens

Immunogen:

Uniprot:

P55263(ADK_HUMAN) >>Visit HPA database.

Gene(ID):

ADK(132)

Expression:

P55263 ADK_HUMAN:

Widely expressed. Highest level in placenta, liver, muscle and kidney.

Sequence:

P55263 ADK_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAAEEEPKPKKLKVEAPQALRENILFGMGNPLLDISAVVDKDFLDKYSLKPNDQILAEDKHKELFDELVKKFKVEYHAGGSTQNSIKVAQWMIQQPHKAATFFGCIGIDKFGEILKRKAAEAHVDAHYYEQNEQPTGTCAACITGDNRSLIANLAAANCYKKEKHLDLEKNWMLVEKARVCYIAGFFLTVSPESVLKVAHHASENNRIFTLNLSAPFISQFYKESLMKVMPYVDILFGNETEAATFAREQGFETKDIKEIAKKTQALPKMNSKRQRIVIFTQGRDDTIMATESEVTAFAVLDQDQKEIIDTNGAGDAFVGGFLSQLVSDKPLTECIRAGHYAASIIIRRTGCTFPEKPDFH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Horse

Chicken

Rabbit

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P55263 As Substrate

P55263

Site	PTM Type	Source
A2	Acetylation	Uniprot
K42	Ubiquitination	Uniprot
K47	Acetylation	Uniprot
K47	Ubiquitination	Uniprot
K51	Ubiquitination	Uniprot
K61	Ubiquitination	Uniprot
K63	Ubiquitination	Uniprot
K71	Ubiquitination	Uniprot
Y77	Phosphorylation	Uniprot
K111	Ubiquitination	Uniprot
K117	Ubiquitination	Uniprot
C160	S-Nitrosylation	Uniprot
K162	Acetylation	Uniprot
K162	Sumoylation	Uniprot
K162	Ubiquitination	Uniprot
K165	Ubiquitination	Uniprot
K171	Ubiquitination	Uniprot
T246	Phosphorylation	Uniprot
K256	Ubiquitination	Uniprot
K259	Ubiquitination	Uniprot
K264	Ubiquitination	Uniprot
K270	Acetylation	Uniprot
K274	Acetylation	Uniprot
C353	S-Nitrosylation	Uniprot

Research Backgrounds

P55263_ADK_HUMAN

Function:

ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.

Subcellular Location:

Nucleus.

Cytoplasm.

Tissue Specificity:

Widely expressed. Highest level in placenta, liver, muscle and kidney.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the carbohydrate kinase PfkB family.

Research Fields

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

ADK Antibody - #DF3218