EFNA2 Antibody | Affinity Biosciences

IF/ICC
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Product:	EFNA2 Antibody
Catalog:	AF0335
Description:	Rabbit polyclonal antibody to EFNA2
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Zebrafish, Bovine, Chicken
Mol.Wt.:	24kDa; 24kD(Calculated).
Uniprot:	O43921
RRID:	AB_2833495

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Zebrafish(82%), Bovine(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

EFNA2 Antibody detects endogenous levels of total EFNA2.

RRID:

AB_2833495
Cite Format: Affinity Biosciences Cat# AF0335, RRID:AB_2833495.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

EFNA 2; efna2; EFNA2_HUMAN; ELF1; Eph related receptor tyrosine kinase ligand 6; EPH-related receptor tyrosine kinase ligand 6; Ephrin-A2; EphrinA2; EPLG6; HEK7 L; HEK7 ligand; HEK7-L; HEK7L; LERK 6; LERK-6; LERK6; Ligand of eph related kinase 6;

Immunogens

Immunogen:

Uniprot:

O43921(EFNA2_HUMAN) >>Visit HPA database.

Gene(ID):

EFNA2(1943)

Description:

EFNA2 Belongs to the ephrin family. Binds to the receptor tyrosine kinases EPHA3, EPHA4 and EPHA5.

Sequence:

O43921 EFNA2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAPAQRPLLPLLLLLLPLPPPPFARAEDAARANSDRYAVYWNRSNPRFHAGAGDDGGGYTVEVSINDYLDIYCPHYGAPLPPAERMEHYVLYMVNGEGHASCDHRQRGFKRWECNRPAAPGGPLKFSEKFQLFTPFSLGFEFRPGHEYYYISATPPNAVDRPCLRLKVYVRPTNETLYEAPEPIFTSNNSCSSPGGCRLFLSTIPVLWTLLGS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Chicken

100

Zebrafish

Horse

Sheep

Dog

Xenopus

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43921 As Substrate

O43921

Site	PTM Type	Enzyme	Source
K125	Acetylation		Uniprot

Research Backgrounds

O43921_EFNA2_HUMAN

Function:

Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. With the EPHA2 receptor may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis (By similarity).

Subcellular Location:

Cell membrane>Lipid-anchor.

Subunit Structure:

Binds to the receptor tyrosine kinases EPHA3, EPHA4 and EPHA5. Interacts with EPHA8; activates EPHA8.

Family&Domains:

Belongs to the ephrin family.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Organismal Systems > Development > Axon guidance. (View pathway)

References

1). Exosomal ephrinA2 derived from serum as a potential biomarker for prostate cancer. Journal of Cancer, 2018 (PubMed: 30087706) [IF=3.9]

Application: WB Species: human Sample: PCa

Fig. 1. |Expression of ephrinA2 in tissues and serum. A. Expression of ephrin A2 protein in 9 pairs of PCa tissues by western blotting (N, adjacent tissue; T, tumor tissue). The result shows the representative western blots in four pairs. B. Expression of ephrinA2 protein in 20 healthy controls, 21 BPH patients and 50 PCa patients measured via ELISA.

2). Ephrin-A2 promotes prostate cancer metastasis by enhancing angiogenesis and promoting EMT. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, 2021 (PubMed: 33772606) [IF=3.6]

Application: WB Species: Human Sample: LNCaP cell

Fig. 1 Ephrin-A2 was assessed in established stable cell lines through Western blotting analysis: a Ephrin-A2 level was detected in PC-3 cell lines (Left panel). The relative expression of ephrin-A2 was normalized to that in PC-3 cells (Right panel). b Ephrin-A2 level was detected in DU145 cell lines (Left panel). Ephrin-A2 relative level expression was normalized to that in DU145/NC cells (Right panel) c Ephrin-A2 level was detected in LNCaP cell lines (Left panel). Ephrin-A2 relative level expression was normalized to that in LNCaP/NC cells (Right panel). *P<0.05 compared with the control group

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For Research Use Only.
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EFNA2 Antibody - #AF0335