Product: DAGK Antibody
Catalog: DF3209
Source: Rabbit
Application: WB, IF/ICC, ELISA(peptide)
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Dog, Chicken, Xenopus
Mol.Wt.: 101 kD; 101kD(Calculated).
Uniprot: P52824
RRID: AB_2835589

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(88%), Dog(100%), Chicken(88%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
DAGK Antibody detects endogenous levels of total DAGK.
RRID:
AB_2835589
Cite Format: Affinity Biosciences Cat# DF3209, RRID:AB_2835589.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DAG kinase theta; DAGK4; DAGK7; DGK theta; DGK-theta; Dgkq; DGKQ_HUMAN; Diacylglycerol kinase delta (110kD); Diacylglycerol kinase theta 110kDa; Diacylglycerol kinase theta; Diglyceride kinase theta;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MAAAAEPGARAWLGGGSPRPGSPACSPVLGSGGRARPGPGPGPGPERAGVRAPGPAAAPGHSFRKVTLTKPTFCHLCSDFIWGLAGFLCDVCNFMSHEKCLKHVRIPCTSVAPSLVRVPVAHCFGPRGLHKRKFCAVCRKVLEAPALHCEVCELHLHPDCVPFACSDCRQCHQDGHQDHDTHHHHWREGNLPSGARCEVCRKTCGSSDVLAGVRCEWCGVQAHSLCSAALAPECGFGRLRSLVLPPACVRLLPGGFSKTQSFRIVEAAEPGEGGDGADGSAAVGPGRETQATPESGKQTLKIFDGDDAVRRSQFRLVTVSRLAGAEEVLEAALRAHHIPEDPGHLELCRLPPSSQACDAWAGGKAGSAVISEEGRSPGSGEATPEAWVIRALPRAQEVLKIYPGWLKVGVAYVSVRVTPKSTARSVVLEVLPLLGRQAESPESFQLVEVAMGCRHVQRTMLMDEQPLLDRLQDIRQMSVRQVSQTRFYVAESRDVAPHVSLFVGGLPPGLSPEEYSSLLHEAGATKATVVSVSHIYSSQGAVVLDVACFAEAERLYMLLKDMAVRGRLLTALVLPDLLHAKLPPDSCPLLVFVNPKSGGLKGRDLLCSFRKLLNPHQVFDLTNGGPLPGLHLFSQVPCFRVLVCGGDGTVGWVLGALEETRYRLACPEPSVAILPLGTGNDLGRVLRWGAGYSGEDPFSVLLSVDEADAVLMDRWTILLDAHEAGSAENDTADAEPPKIVQMSNYCGIGIDAELSLDFHQAREEEPGKFTSRLHNKGVYVRVGLQKISHSRSLHKQIRLQVERQEVELPSIEGLIFINIPSWGSGADLWGSDSDTRFEKPRMDDGLLEVVGVTGVVHMGQVQGGLRSGIRIAQGSYFRVTLLKATPVQVDGEPWVQAPGHMIISAAGPKVHMLRKAKQKPRRAGTTRDARADAAPAPESDPR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Horse
88
Xenopus
88
Chicken
88
Sheep
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P52824 As Substrate

Site PTM Type Enzyme
S17 Phosphorylation
S22 Phosphorylation
S26 Phosphorylation
R51 Methylation
K202 Ubiquitination
K258 Ubiquitination
K297 Ubiquitination
S376 Phosphorylation
K400 Ubiquitination
Y412 Phosphorylation
T835 Phosphorylation

Research Backgrounds

Function:

Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). May regulate the activity of protein kinase C by controlling the balance between these two signaling lipids. Activated in the nucleus in response to alpha-thrombin and nerve growth factor (By similarity). May be involved in cAMP-induced activation of NR5A1 and subsequent steroidogenic gene transcription by delivering PA as ligand for NR5A1. Acts synergistically with NR5A1 on CYP17 transcriptional activity.

PTMs:

Phosphorylated by PRKCE and PRKCH in vitro.

Subcellular Location:

Cytoplasm. Cell membrane. Cytoplasm>Cytoskeleton. Nucleus. Nucleus speckle.
Note: Translocates to the nucleus in response to thrombin stimulation (PubMed:15632189). Translocates to the plasma membrane in response to steroid hormone receptor stimulation (PubMed:15632189). Translocation to the plasma membrane is dependent on G-protein coupled receptor stimulation and subsequent activation of PRKCE and probably PRKCH (PubMed:15632189).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with RHOA (constitutively activated, GTP-bound); the interaction inhibits DGKQ. Interacts with PRKCE. Interacts with PRKCH. Interacts with PLCB1. Interacts with NR5A1; the interaction requires the LXXLL motif 1 and LXXLL motif 2 in DGKQ.

Family&Domains:

The L-X-X-L-L repeats are implicated in binding to the nuclear receptor NR5A1.

Belongs to the eukaryotic diacylglycerol kinase family.

Research Fields

· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Metabolism > Lipid metabolism > Glycerolipid metabolism.

· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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