DGKE Antibody | Affinity Biosciences

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Western blot analysis of extracts from K562 cells, using DGKE antibody.

Product:	DGKE Antibody
Catalog:	DF3203
Description:	Rabbit polyclonal antibody to DGKE
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.:	60 KD; 64kD(Calculated).
Uniprot:	P52429
RRID:	AB_2835583

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(91%), Horse(100%), Sheep(91%), Dog(100%), Chicken(91%)

Clonality:

Polyclonal

Specificity:

DGKE Antibody detects endogenous levels of total DGKE.

RRID:

AB_2835583
Cite Format: Affinity Biosciences Cat# DF3203, RRID:AB_2835583.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AHUS7; DAG kinase epsilon; DAGK 5; DAGK 6; DAGK5; DAGK6; DGK; DGK epsilon; DGK-epsilon; DGKE; DGKE_HUMAN; Diacylglycerol kinase epsilon 64kDa; Diacylglycerol kinase epsilon; Diglyceride kinase epsilon; NPHS7;

Immunogens

Immunogen:

Uniprot:

P52429(DGKE_HUMAN) >>Visit HPA database.

Gene(ID):

DGKE(8526)

Expression:

P52429 DGKE_HUMAN:

Expressed predominantly in testis. Expressed in endothelium, platelets and podocytes (at protein level).

Sequence:

P52429 DGKE_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEAERRPAPGSPSEGLFADGHLILWTLCSVLLPVFITFWCSLQRSRRQLHRRDIFRKSKHGWRDTDLFSQPTYCCVCAQHILQGAFCDCCGLRVDEGCLRKADKRFQCKEIMLKNDTKVLDAMPHHWIRGNVPLCSYCMVCKQQCGCQPKLCDYRCIWCQKTVHDECMKNSLKNEKCDFGEFKNLIIPPSYLTSINQMRKDKKTDYEVLASKLGKQWTPLIILANSRSGTNMGEGLLGEFRILLNPVQVFDVTKTPPIKALQLCTLLPYYSARVLVCGGDGTVGWVLDAVDDMKIKGQEKYIPQVAVLPLGTGNDLSNTLGWGTGYAGEIPVAQVLRNVMEADGIKLDRWKVQVTNKGYYNLRKPKEFTMNNYFSVGPDALMALNFHAHREKAPSLFSSRILNKAVYLFYGTKDCLVQECKDLNKKVELELDGERVALPSLEGIIVLNIGYWGGGCRLWEGMGDETYPLARHDDGLLEVVGVYGSFHCAQIQVKLANPFRIGQAHTVRLILKCSMMPMQVDGEPWAQGPCTVTITHKTHAMMLYFSGEQTDDDISSTSDQEDIKATE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Dog

100

Bovine

Sheep

Chicken

Rabbit

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P52429 As Substrate

P52429

Site	PTM Type	Source
K101	Acetylation	Uniprot
K109	Ubiquitination	Uniprot
K169	Ubiquitination	Uniprot
K176	Ubiquitination	Uniprot
K203	Ubiquitination	Uniprot
Y206	Phosphorylation	Uniprot
K212	Ubiquitination	Uniprot
K215	Ubiquitination	Uniprot
K254	Ubiquitination	Uniprot
T265	Phosphorylation	Uniprot
K351	Ubiquitination	Uniprot
K357	Ubiquitination	Uniprot
K392	Ubiquitination	Uniprot
K404	Ubiquitination	Uniprot
K413	Ubiquitination	Uniprot

Research Backgrounds

P52429_DGKE_HUMAN

Function:

Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition.

Subcellular Location:

Membrane>Multi-pass membrane protein. Membrane. Cytoplasm.

Tissue Specificity:

Expressed predominantly in testis. Expressed in endothelium, platelets and podocytes (at protein level).

Family&Domains:

Belongs to the eukaryotic diacylglycerol kinase family.

Research Fields

· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)

· Metabolism > Lipid metabolism > Glycerolipid metabolism.

· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

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For Research Use Only.
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DGKE Antibody - #DF3203