TOP3A Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from 293 cells, using TOP3A antibody.

Product:	TOP3A Antibody
Catalog:	DF3265
Description:	Rabbit polyclonal antibody to TOP3A
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Rabbit, Dog, Xenopus
Mol.Wt.:	115 KD; 112kD(Calculated).
Uniprot:	Q13472
RRID:	AB_2835563

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Rabbit(88%), Dog(89%), Xenopus(88%)

Clonality:

Polyclonal

Specificity:

TOP3A Antibody detects endogenous levels of total TOP3A.

RRID:

AB_2835563
Cite Format: Affinity Biosciences Cat# DF3265, RRID:AB_2835563.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DNA topoisomerase 3 alpha; DNA topoisomerase 3-alpha; DNA topoisomerase III alpha; TOP 3; TOP3; TOP3A; TOP3A_HUMAN; topo III-alpha; topoisomerase (DNA) III alpha; topoisomerase (DNA) III; topoisomerase III alpha; ZGRF7; zinc finger, GRF-type containing 7;

Immunogens

Immunogen:

Uniprot:

Q13472(TOP3A_HUMAN) >>Visit HPA database.

Gene(ID):

TOP3A(7156)

Expression:

Q13472 TOP3A_HUMAN:

High expression is found in testis, heart, skeletal muscle and pancreas.

Sequence:

Q13472 TOP3A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MIFPVARYALRWLRRPEDRAFSRAAMEMALRGVRKVLCVAEKNDAAKGIADLLSNGRMRRREGLSKFNKIYEFDYHLYGQNVTMVMTSVSGHLLAHDFQMQFRKWQSCNPLVLFEAEIEKYCPENFVDIKKTLERETRQCQALVIWTDCDREGENIGFEIIHVCKAVKPNLQVLRARFSEITPHAVRTACENLTEPDQRVSDAVDVRQELDLRIGAAFTRFQTLRLQRIFPEVLAEQLISYGSCQFPTLGFVVERFKAIQAFVPEIFHRIKVTHDHKDGIVEFNWKRHRLFNHTACLVLYQLCVEDPMATVVEVRSKPKSKWRPQALDTVELEKLASRKLRINAKETMRIAEKLYTQGYISYPRTETNIFPRDLNLTVLVEQQTPDPRWGAFAQSILERGGPTPRNGNKSDQAHPPIHPTKYTNNLQGDEQRLYEFIVRHFLACCSQDAQGQETTVEIDIAQERFVAHGLMILARNYLDVYPYDHWSDKILPVYEQGSHFQPSTVEMVDGETSPPKLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLTPDKRFLPGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICDGKKDKFVVLRQQVQKYKQVFIEAVAKAKKLDEALAQYFGNGTELAQQEDIYPAMPEPIRKCPQCNKDMVLKTKKNGGFYLSCMGFPECRSAVWLPDSVLEASRDSSVCPVCQPHPVYRLKLKFKRGSLPPTMPLEFVCCIGGCDDTLREILDLRFSGGPPRASQPSGRLQANQSLNRMDNSQHPQPADSRQTGSSKALAQTLPPPTAAGESNSVTCNCGQEAVLLTVRKEGPNRGRQFFKCNGGSCNFFLWADSPNPGAGGPPALAYRPLGASLGCPPGPGIHLGGFGNPGDGSGSGTSCLCSQPSVTRTVQKDGPNKGRQFHTCAKPREQQCGFFQWVDENTAPGTSGAPSWTGDRGRTLESEARSKRPRASSSDMGSTAKKPRKCSLCHQPGHTRPFCPQNR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

Xenopus

Rabbit

Pig

Horse

Bovine

Sheep

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13472 As Substrate

Q13472

Site	PTM Type	Source
S22	Phosphorylation	Uniprot
K35	Ubiquitination	Uniprot
K42	Ubiquitination	Uniprot
K47	Ubiquitination	Uniprot
S54	Phosphorylation	Uniprot
K66	Ubiquitination	Uniprot
K130	Ubiquitination	Uniprot
K168	Ubiquitination	Uniprot
K257	Ubiquitination	Uniprot
K321	Ubiquitination	Uniprot
K334	Ubiquitination	Uniprot
K345	Ubiquitination	Uniprot
K353	Ubiquitination	Uniprot
K421	Ubiquitination	Uniprot
K529	Ubiquitination	Uniprot
K546	Ubiquitination	Uniprot
T554	Phosphorylation	Uniprot
K557	Ubiquitination	Uniprot
K602	Ubiquitination	Uniprot
K668	Acetylation	Uniprot
T669	Phosphorylation	Uniprot
K670	Acetylation	Uniprot
K671	Ubiquitination	Uniprot
S694	Phosphorylation	Uniprot
S703	Phosphorylation	Uniprot
S760	Phosphorylation	Uniprot
S771	Phosphorylation	Uniprot
S778	Phosphorylation	Uniprot
S964	Phosphorylation	Uniprot
S970	Phosphorylation	Uniprot
S971	Phosphorylation	Uniprot
S972	Phosphorylation	Uniprot
S976	Phosphorylation	Uniprot
T977	Phosphorylation	Uniprot
S985	Phosphorylation	Uniprot

Research Backgrounds

Q13472_TOP3A_HUMAN

Function:

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. As an essential component of the RMI complex it is involved in chromosome separation and the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity. It is required for mtDNA decatenation and segregation after completion of replication, in a process that does not require BLM, RMI1 and RMI2.

Subcellular Location:

Mitochondrion matrix.

Tissue Specificity:

High expression is found in testis, heart, skeletal muscle and pancreas.

Subunit Structure:

Binds ssDNA. Interacts (via N-terminal region) with BLM; the interaction is direct. Directly interacts with RMI1. Component of the RMI complex, containing at least TOP3A, RMI1 and RMI2. The RMI complex interacts with BLM.

Family&Domains:

Belongs to the type IA topoisomerase family.

Research Fields

· Genetic Information Processing > Replication and repair > Homologous recombination.

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

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TOP3A Antibody - #DF3265