SNAP25 Recombinant Rabbit mAb - #BF3844

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Product: SNAP25 Recombinant Rabbit mAb
Catalog: BF3844
Description: Rabbit monoclonal antibody to SNAP25
Application: WB IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 25 kDa(Observed); 23kD(Calculated).
Uniprot: P60880

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 100ul $280 In stock

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Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit IgG
Application:
WB 1:1000-1:5000, IHC 1:100-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Monoclonal [ReFirm23291]
Specificity:
SNAP25 Recombinant Rabbit mAb detects endogenous levels of SNAP25.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

bA416N4.2; Bdr; CMS18; dJ1068F16.2; FLJ23079; HGNC:11132; MGC105414; MGC139754; Resistance to inhibitors of cholinesterase 4 homolog; RIC 4; RIC4; SEC 9; SEC9; SNAP 25; SNAP; SNAP-25; SNAP-25B; SNAP25; SNP 25; SNP25; SNP25_HUMAN; sp; SUP; Super protein; Synaptosomal associated 25 kDa protein; Synaptosomal associated protein; Synaptosomal associated protein 25; Synaptosomal associated protein 25kDa; Synaptosomal-associated 25 kDa protein; Synaptosomal-associated protein 25; Synaptosomal-associated protein, 25-KD;

Immunogens

Immunogen:

A synthetic peptide from human SNAP25

Uniprot:

P60880(SNP25_HUMAN) >>Visit HPA database.

Gene(ID):
SNAP25(6616)
Expression:
P60880 SNP25_HUMAN:

Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.

Description:
Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE,t-SNARE complex consists of a bundle of four helices,one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane,the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore,this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene.
Sequence:
MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG

Research Backgrounds

Function:

t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.

PTMs:

Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association (By similarity).

(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type A (BoNT/A, botA) which hydrolyzes the 197-Gln-|-Arg-198 bond and inhibits neurotransmitter release.

(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type C (BoNT/C) which hydrolyzes the 198-Arg-|-Ala-199 bond and inhibits neurotransmitter release. C.botulinum type C only rarely infects humans.

(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type E (BoNT/E) which hydrolyzes the 180-Arg-|-Ile-181 bond and inhibits neurotransmitter release.

Subcellular Location:

Cytoplasm>Perinuclear region. Cell membrane>Lipid-anchor. Cell junction>Synapse>Synaptosome. Photoreceptor inner segment.
Note: Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region. Colocalizes with KCNB1 at the cell membrane (By similarity). Colocalizes with PLCL1 at the cell membrane (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.

Family&Domains:

Belongs to the SNAP-25 family.

Research Fields

· Organismal Systems > Nervous system > Synaptic vesicle cycle.

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

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