Product: BUD31 Antibody
Catalog: DF3175
Description: Rabbit polyclonal antibody to BUD31
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 17 KD; 17kD(Calculated).
Uniprot: P41223
RRID: AB_2835551

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
BUD31 Antibody detects endogenous levels of total BUD31.
RRID:
AB_2835551
Cite Format: Affinity Biosciences Cat# DF3175, RRID:AB_2835551.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Bud31; BUD31 homolog (S. cerevisiae); BUD31_HUMAN; Cwc14; EDG 2; EDG2; fSAP17; Functional spliceosome associated protein 17; G10; G10 maternal transcript homolog; Maternal G10 transcript; Protein BUD31 homolog; Protein EDG-2; Protein G10 homolog; YCR063W;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P41223 BUD31_HUMAN:

Detected in epithelial and stromal cells in benign prostate hyperplasia tissue (at protein level).

Sequence:
MPKVKRSRKAPPDGWELIEPTLDELDQKMREAETEPHEGKRKVESLWPIFRIHHQKTRYIFDLFYKRKAISRELYEYCIKEGYADKNLIAKWKKQGYENLCCLRCIQTRDTNFGTNCICRVPKSKLEVGRIIECTHCGCRGCSG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
91
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P41223 As Substrate

Site PTM Type Enzyme
K9 Ubiquitination
K28 Ubiquitination
K40 Ubiquitination
K42 Sumoylation
K42 Ubiquitination
K66 Ubiquitination
K80 Ubiquitination
K86 Ubiquitination
K91 Ubiquitination
K94 Ubiquitination
Y97 Phosphorylation
T115 Phosphorylation
K123 Ubiquitination
K125 Acetylation
K125 Sumoylation
K125 Ubiquitination
R140 Methylation

Research Backgrounds

Function:

Involved in the pre-mRNA splicing process. May play a role as regulator of AR transcriptional activity; may increase AR transcriptional activity.

Subcellular Location:

Nucleus.
Note: Detected in chromatin at the promoter of AR target genes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in epithelial and stromal cells in benign prostate hyperplasia tissue (at protein level).

Subunit Structure:

Identified in the spliceosome C complex. May interact with AR.

Family&Domains:

Contains a short sequence motif (Phe-Xaa-Xaa-Phe-Tyr) that can bind to AR and may modulate AR activity.

Belongs to the BUD31 (G10) family.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

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