GCLM Recombinant Rabbit mAb - #BF3676

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Product: GCLM Recombinant Rabbit mAb
Catalog: BF3676
Description: Rabbit monoclonal antibody to GCLM
Application: WB IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 31 kDa(Observed); 31kD(Calculated).
Uniprot: P48507

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 100ul $280 In stock

Lead Time: Same day delivery

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MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit IgG
Application:
WB 1:1000-1:5000, IHC 1:50-1:100
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Monoclonal [ReFirm23123]
Specificity:
GCLM Recombinant Rabbit mAb detects endogenous levels of GCLM.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Gamma ECS regulatory subunit; Gamma-ECS regulatory subunit; Gamma-glutamylcysteine synthetase regulatory subunit; GCLM; GCS light chain; GLCLR; Glutamate cysteine ligase regulatory subunit; Glutamate--cysteine ligase modifier subunit; Glutamate--cysteine ligase regulatory subunit; GSC light chain; GSH0_HUMAN;

Immunogens

Immunogen:

A synthetic peptide from human GCLM

Uniprot:

P48507(GSH0_HUMAN) >>Visit HPA database.

Gene(ID):
GCLM(2730)
Expression:
P48507 GSH0_HUMAN:

In all tissues examined. Highest levels in skeletal muscle.

Description:
Glutamate-cysteine ligase,also known as gamma-glutamylcysteine synthetase,is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits,a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms.
Sequence:
MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS

Research Backgrounds

Tissue Specificity:

In all tissues examined. Highest levels in skeletal muscle.

Family&Domains:

Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

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For Research Use Only.
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