GRK3 Antibody - #DF3165

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Product: GRK3 Antibody
Catalog: DF3165
Description: Rabbit polyclonal antibody to GRK3
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 80 KD; 80kD(Calculated).
Uniprot: P35626
RRID: AB_2835541

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
GRK3 Antibody detects endogenous levels of total GRK3.
RRID:
AB_2835541
Cite Format: Affinity Biosciences Cat# DF3165, RRID:AB_2835541.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ADRBK2; Adrenergic, beta, receptor kinase 2; ARBK2_HUMAN; BARK2; Beta adrenergic receptor kinase 2; Beta ARK 2; Beta-adrenergic receptor kinase 2; Beta-ARK-2; EC 2.7.11.15; G protein coupled receptor kinase 3; G-protein-coupled receptor kinase 3; GRK3;

Immunogens

Immunogen:
Uniprot:

P35626(ARBK2_HUMAN) >>Visit HPA database.

Gene(ID):
GRK3(157)
Sequence:
MADLEAVLADVSYLMAMEKSKATPAARASKRIVLPEPSIRSVMQKYLAERNEITFDKIFNQKIGFLLFKDFCLNEINEAVPQVKFYEEIKEYEKLDNEEDRLCRSRQIYDAYIMKELLSCSHPFSKQAVEHVQSHLSKKQVTSTLFQPYIEEICESLRGDIFQKFMESDKFTRFCQWKNVELNIHLTMNEFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMTYAFHTPDKLCFILDLMNGGDLHYHLSQHGVFSEKEMRFYATEIILGLEHMHNRFVVYRDLKPANILLDEHGHARISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGTAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTVNVELPDTFSPELKSLLEGLLQRDVSKRLGCHGGGSQEVKEHSFFKGVDWQHVYLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDCDQELYKNFPLVISERWQQEVTETVYEAVNADTDKIEARKRAKNKQLGHEEDYALGKDCIMHGYMLKLGNPFLTQWQRRYFYLFPNRLEWRGEGESRQNLLTMEQILSVEETQIKDKKCILFRIKGGKQFVLQCESDPEFVQWKKELNETFKEAQRLLRRAPKFLNKPRSGTVELPKPSLCHRNSNGL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35626 As Substrate

Site PTM Type Enzyme
K45 Ubiquitination
K57 Ubiquitination
K62 Ubiquitination
K84 Ubiquitination
K90 Ubiquitination
K94 Ubiquitination
Y109 Phosphorylation
Y112 Phosphorylation
K115 Ubiquitination
K126 Ubiquitination
K138 Ubiquitination
K139 Ubiquitination
K164 Ubiquitination
K170 Ubiquitination
Y206 Phosphorylation
T213 Phosphorylation
K215 Ubiquitination
K220 Ubiquitination
K230 Ubiquitination
K319 Ubiquitination
K344 Ubiquitination
K345 Ubiquitination
K346 Ubiquitination
S350 Phosphorylation
T353 Phosphorylation
Y356 Phosphorylation
S423 Phosphorylation
K448 Ubiquitination
K454 Ubiquitination
K465 Ubiquitination
S487 Phosphorylation
K494 Ubiquitination
K497 Ubiquitination
K507 Ubiquitination
S514 Phosphorylation
T524 Phosphorylation
Y526 Phosphorylation
K545 Ubiquitination
Y564 Phosphorylation
T612 Phosphorylation
K625 Acetylation
K628 Acetylation
K628 Ubiquitination
K644 Ubiquitination
K645 Ubiquitination
K652 Ubiquitination
S670 Phosphorylation
T672 Phosphorylation
S685 Phosphorylation

PTMs - P35626 As Enzyme

Substrate Site Source
P07550 (ADRB2) S355 Uniprot
P07550 (ADRB2) S356 Uniprot
P21731-2 (TBXA2R) S239 Uniprot
P21731-2 (TBXA2R) S357 Uniprot
P30411 (BDKRB2) S366 Uniprot
P30411 (BDKRB2) S373 Uniprot
P30411 (BDKRB2) S375 Uniprot
P30874 (SSTR2) S341 Uniprot
P30874 (SSTR2) S343 Uniprot
P35372 (OPRM1) T182 Uniprot
P41146 (OPRL1) S363 Uniprot
P51681 (CCR5) S336 Uniprot
P51681 (CCR5) S337 Uniprot
P51681 (CCR5) S342 Uniprot
P51681 (CCR5) S349 Uniprot

Research Backgrounds

Function:

Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors.

PTMs:

Ubiquitinated.

Family&Domains:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway.   (View pathway)

· Human Diseases > Substance dependence > Morphine addiction.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Glutamatergic synapse.

· Organismal Systems > Sensory system > Olfactory transduction.

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