GRK5 Antibody - #DF3159
| Product: | GRK5 Antibody |
| Catalog: | DF3159 |
| Description: | Rabbit polyclonal antibody to GRK5 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Dog, Chicken |
| Mol.Wt.: | 68 KD; 68kD(Calculated). |
| Uniprot: | P34947 |
| RRID: | AB_2835536 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF3159, RRID:AB_2835536.
Fold/Unfold
FLJ39780; FP2025; G protein coupled receptor kinase 5; G protein coupled receptor kinase GRK5; G protein-coupled receptor kinase 5; G protein-coupled receptor kinase GRK5; GPRK5; GRK-pan; GRK5; GRK5_HUMAN; Pan-GRK;
Immunogens
Highest levels in heart, placenta, lung > skeletal muscle > brain, liver, pancreas > kidney.
- P34947 GRK5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MELENIVANTVLLKAREGGGGKRKGKSKKWKEILKFPHISQCEDLRRTIDRDYCSLCDKQPIGRLLFRQFCETRPGLECYIQFLDSVAEYEVTPDEKLGEKGKEIMTKYLTPKSPVFIAQVGQDLVSQTEEKLLQKPCKELFSACAQSVHEYLRGEPFHEYLDSMFFDRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKRLEKKRIKKRKGESMALNEKQILEKVNSQFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNPGFEEERALFYAAEILCGLEDLHRENTVYRDLKPENILLDDYGHIRISDLGLAVKIPEGDLIRGRVGTVGYMAPEVLNNQRYGLSPDYWGLGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSHKFSEEAKSICKMLLTKDAKQRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDPRAVYCKDVLDIEQFSTVKGVNLDHTDDDFYSKFSTGSVSIPWQNEMIETECFKELNVFGPNGTLPPDLNRNHPPEPPKKGLLQRLFKRQHQNNSKSSPSSKTSFNHHINSNHVSSNSTGSS
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P34947 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T10 | Phosphorylation | Uniprot | |
| K14 | Ubiquitination | Uniprot | |
| K24 | Acetylation | Uniprot | |
| K26 | Acetylation | Uniprot | |
| S27 | Phosphorylation | Uniprot | |
| K28 | Acetylation | Uniprot | |
| K29 | Acetylation | Uniprot | |
| S40 | Phosphorylation | Uniprot | |
| Y90 | Phosphorylation | P09619 (PDGFRB) | Uniprot |
| Y109 | Phosphorylation | P09619 (PDGFRB) | Uniprot |
| K113 | Ubiquitination | Uniprot | |
| K183 | Ubiquitination | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| K210 | Ubiquitination | Uniprot | |
| K226 | Acetylation | Uniprot | |
| K235 | Ubiquitination | Uniprot | |
| Y251 | Phosphorylation | P12931 (SRC) | Uniprot |
| Y253 | Phosphorylation | P12931 (SRC) | Uniprot |
| Y309 | Phosphorylation | P09619 (PDGFRB) | Uniprot |
| Y368 | Phosphorylation | P09619 (PDGFRB) | Uniprot |
| Y408 | Phosphorylation | Uniprot | |
| K411 | Ubiquitination | Uniprot | |
| K443 | Ubiquitination | Uniprot | |
| S484 | Phosphorylation | P34947 (GRK5) | Uniprot |
| T485 | Phosphorylation | P34947 (GRK5) | Uniprot |
| K487 | Ubiquitination | Uniprot | |
| K548 | Methylation | Uniprot | |
| S586 | Phosphorylation | Uniprot |
PTMs - P34947 As Enzyme
| Substrate | Site | Source |
|---|---|---|
| O75581 (LRP6) | S1448 | Uniprot |
| O75581 (LRP6) | S1450 | Uniprot |
| O75581 (LRP6) | S1490 | Uniprot |
| O75581 (LRP6) | S1508 | Uniprot |
| O75581 (LRP6) | T1572 | Uniprot |
| O75581 (LRP6) | S1590 | Uniprot |
| O75581 (LRP6) | S1607 | Uniprot |
| O76070 (SNCG) | S124 | Uniprot |
| P04637 (TP53) | T55 | Uniprot |
| P06748 (NPM1) | S4 | Uniprot |
| P07550 (ADRB2) | S355 | Uniprot |
| P07550 (ADRB2) | S356 | Uniprot |
| P07550 (ADRB2) | T384 | Uniprot |
| P07550 (ADRB2) | T393 | Uniprot |
| P07550 (ADRB2) | S396 | Uniprot |
| P07550 (ADRB2) | S401 | Uniprot |
| P07550 (ADRB2) | S407 | Uniprot |
| P07550 (ADRB2) | S411 | Uniprot |
| P25963 (NFKBIA) | S32 | Uniprot |
| P26038 (MSN) | T66 | Uniprot |
| P30411 (BDKRB2) | S366 | Uniprot |
| P30411 (BDKRB2) | S373 | Uniprot |
| P30411-1 (BDKRB2) | S375 | Uniprot |
| P34947 (GRK5) | S484 | Uniprot |
| P34947 (GRK5) | T485 | Uniprot |
| P37840-1 (SNCA) | S129 | Uniprot |
| P49407 (ARRB1) | S412 | Uniprot |
| P50502 (ST13) | S346 | Uniprot |
| P61073 (CXCR4) | S339 | Uniprot |
| Q16143 (SNCB) | S118 | Uniprot |
Research Backgrounds
Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro).
Autophosphorylated. Autophosphorylation may play a critical role in the regulation of GRK5 kinase activity.
Cytoplasm. Nucleus. Cell membrane>Peripheral membrane protein.
Note: Predominantly localized at the plasma membrane; targeted to the cell surface through the interaction with phospholipids. Nucleus localization is regulated in a GPCR and Ca(2+)/calmodulin-dependent fashion.
Highest levels in heart, placenta, lung > skeletal muscle > brain, liver, pancreas > kidney.
Interacts with ST13 (via the C-terminus 303-319 AA). Interacts with TP53/p53. Interacts with HTR4 (via C-terminus 330-346 AA); this interaction is promoted by 5-HT (serotonin). Interacts with HDAC5 (By similarity).
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Research Fields
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Human Diseases > Substance dependence > Morphine addiction.
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.