CSTF2 Antibody - #DF3158

(3)  
Product: CSTF2 Antibody
Catalog: DF3158
Description: Rabbit polyclonal antibody to CSTF2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 60 KD; 61kD(Calculated).
Uniprot: P33240
RRID: AB_2835535

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
CSTF2 Antibody detects endogenous levels of total CSTF2.
RRID:
AB_2835535
Cite Format: Affinity Biosciences Cat# DF3158, RRID:AB_2835535.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

betaCstF 64 variant 2; CF-1 64 kDa subunit; CF1 64 kDa subunit; Cleavage stimulation factor 64 kDa subunit; Cleavage stimulation factor subunit 2; Cleavage stimulation factor, 3' pre RNA, subunit 2, 64kDa; CSTF 2; CstF 64; CSTF 64 kDa subunit; CstF-64; CSTF2; CSTF2_HUMAN; MGC188397;

Immunogens

Immunogen:
Uniprot:

P33240(CSTF2_HUMAN) >>Visit HPA database.

Gene(ID):
CSTF2(1478)
Sequence:
MAGLTVRDPAVDRSLRSVFVGNIPYEATEEQLKDIFSEVGPVVSFRLVYDRETGKPKGYGFCEYQDQETALSAMRNLNGREFSGRALRVDNAASEKNKEELKSLGTGAPVIESPYGETISPEDAPESISKAVASLPPEQMFELMKQMKLCVQNSPQEARNMLLQNPQLAYALLQAQVVMRIVDPEIALKILHRQTNIPTLIAGNPQPVHGAGPGSGSNVSMNQQNPQAPQAQSLGGMHVNGAPPLMQASMQGGVPAPGQMPAAVTGPGPGSLAPGGGMQAQVGMPGSGPVSMERGQVPMQDPRAAMQRGSLPANVPTPRGLLGDAPNDPRGGTLLSVTGEVEPRGYLGPPHQGPPMHHVPGHESRGPPPHELRGGPLPEPRPLMAEPRGPMLDQRGPPLDGRGGRDPRGIDARGMEARAMEARGLDARGLEARAMEARAMEARAMEARAMEARAMEVRGMEARGMDTRGPVPGPRGPIPSGMQGPSPINMGAVVPQGSRQVPVMQGTGMQGASIQGGSQPGGFSPGQNQVTPQDHEKAALIMQVLQLTADQIAMLPPEQRQSILILKEQIQKSTGAP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P33240 As Substrate

Site PTM Type Enzyme
S14 Phosphorylation
K96 Ubiquitination
S113 Phosphorylation
Y115 Phosphorylation
T118 Phosphorylation
S120 Phosphorylation
K130 Ubiquitination
K145 Ubiquitination
K148 Ubiquitination
S154 Phosphorylation
K189 Ubiquitination
R303 Methylation
R308 Methylation
S310 Phosphorylation
T317 Phosphorylation
R319 Methylation
R330 Methylation
T333 Phosphorylation
S336 Phosphorylation
T338 Phosphorylation
R344 Methylation
R365 Methylation
R373 Methylation
R381 Methylation
R388 Methylation
R402 Methylation
R405 Methylation
R423 Methylation
R428 Methylation
R458 Methylation
R463 Methylation
R468 Methylation
R475 Methylation
S480 Phosphorylation
S486 Phosphorylation
S498 Phosphorylation
R499 Methylation
T507 Phosphorylation
S518 Phosphorylation
S524 Phosphorylation
T531 Phosphorylation
S562 Phosphorylation
K567 Ubiquitination
K572 Ubiquitination

Research Backgrounds

Function:

One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs (By similarity).

Subcellular Location:

Nucleus.
Note: Localized with DDX1 in cleavage bodies.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). CSTF2 directly interacts with CSTF3, SYMPK and RPO2TC1. Interacts with HSF1 in heat-stressed cells. Interacts with CPSF2, CPSF3 and FIP1L1. Interacts with DDX1.

Research Fields

· Genetic Information Processing > Translation > mRNA surveillance pathway.

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