CIITA Antibody - #DF3157
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF3157, RRID:AB_2835534.
Fold/Unfold
C2TA; C2TA_HUMAN; CIITA; CIITA IV; Class II major histocompatibility complex transactivator; Class II transactivator; MHC class II transactivator; MHC class II transactivator type III; MHC2TA; NLR family acid domain containing; NLRA; Nucleotide binding oligomerization domain leucine rich repeat and acid domain containing;
Immunogens
- P33076 C2TA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRCLAPRPAGSYLSEPQGSSQCATMELGPLEGGYLELLNSDADPLCLYHFYDQMDLAGEEEIELYSEPDTDTINCDQFSRLLCDMEGDEETREAYANIAELDQYVFQDSQLEGLSKDIFKHIGPDEVIGESMEMPAEVGQKSQKRPFPEELPADLKHWKPAEPPTVVTGSLLVRPVSDCSTLPCLPLPALFNQEPASGQMRLEKTDQIPMPFSSSSLSCLNLPEGPIQFVPTISTLPHGLWQISEAGTGVSSIFIYHGEVPQASQVPPPSGFTVHGLPTSPDRPGSTSPFAPSATDLPSMPEPALTSRANMTEHKTSPTQCPAAGEVSNKLPKWPEPVEQFYRSLQDTYGAEPAGPDGILVEVDLVQARLERSSSKSLERELATPDWAERQLAQGGLAEVLLAAKEHRRPRETRVIAVLGKAGQGKSYWAGAVSRAWACGRLPQYDFVFSVPCHCLNRPGDAYGLQDLLFSLGPQPLVAADEVFSHILKRPDRVLLILDGFEELEAQDGFLHSTCGPAPAEPCSLRGLLAGLFQKKLLRGCTLLLTARPRGRLVQSLSKADALFELSGFSMEQAQAYVMRYFESSGMTEHQDRALTLLRDRPLLLSHSHSPTLCRAVCQLSEALLELGEDAKLPSTLTGLYVGLLGRAALDSPPGALAELAKLAWELGRRHQSTLQEDQFPSADVRTWAMAKGLVQHPPRAAESELAFPSFLLQCFLGALWLALSGEIKDKELPQYLALTPRKKRPYDNWLEGVPRFLAGLIFQPPARCLGALLGPSAAASVDRKQKVLARYLKRLQPGTLRARQLLELLHCAHEAEEAGIWQHVVQELPGRLSFLGTRLTPPDAHVLGKALEAAGQDFSLDLRSTGICPSGLGSLVGLSCVTRFRAALSDTVALWESLQQHGETKLLQAAEEKFTIEPFKAKSLKDVEDLGKLVQTQRTRSSSEDTAGELPAVRDLKKLEFALGPVSGPQAFPKLVRILTAFSSLQHLDLDALSENKIGDEGVSQLSATFPQLKSLETLNLSQNNITDLGAYKLAEALPSLAASLLRLSLYNNCICDVGAESLARVLPDMVSLRVMDVQYNKFTAAGAQQLAASLRRCPHVETLAMWTPTIPFSVQEHLQQQDSRISLR
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P33076 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K141 | Acetylation | Uniprot | |
| K144 | Acetylation | Uniprot | |
| S280 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| S286 | Phosphorylation | Uniprot | |
| S288 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| S293 | Phosphorylation | Uniprot | |
| K315 | Ubiquitination | Uniprot | |
| K330 | Ubiquitination | Uniprot | |
| K333 | Ubiquitination | Uniprot | |
| S373 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S427 | Phosphorylation | Uniprot | |
| Y428 | Phosphorylation | Uniprot | |
| S434 | Phosphorylation | Uniprot | |
| T546 | Phosphorylation | Uniprot | |
| S834 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| T838 | Phosphorylation | Uniprot | |
| T947 | Phosphorylation | Uniprot | |
| K959 | Acetylation | Uniprot | |
| S1050 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S1073 | Phosphorylation | Uniprot |
Research Backgrounds
Essential for transcriptional activity of the HLA class II promoter; activation is via the proximal promoter. No DNA binding of in vitro translated CIITA was detected. May act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter. Also mediates enhanced MHC class I transcription; the promoter element requirements for CIITA-mediated transcription are distinct from those of constitutive MHC class I transcription, and CIITA can functionally replace TAF1 at these genes. Exhibits intrinsic GTP-stimulated acetyltransferase activity. Exhibits serine/threonine protein kinase activity: can phosphorylate the TFIID component TAF7, the RAP74 subunit of the general transcription factor TFIIF, histone H2B at 'Ser-37' and other histones (in vitro).
Autophosphorylated, affecting interaction with TAF7.
Nucleus. Nucleus>PML body.
Note: Recruited to PML body by PML.
Interacts with ZXDA and ZXDC. Interacts with PML (isoform PML-2).
The acetyltransferase domain is necessary for activation of both class I and class II transcription.
The GTP-binding motif doesn't confer GTPase activity but promotes nuclear localization.
Research Fields
· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Immune diseases > Primary immunodeficiency.
· Organismal Systems > Immune system > Antigen processing and presentation. (View pathway)
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