CD153 Antibody | Affinity Biosciences

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Western blot analysis of extracts from RAW264.

Product:	CD153 Antibody
Catalog:	DF3156
Description:	Rabbit polyclonal antibody to CD153
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	38 KD; 26kD(Calculated).
Uniprot:	P32971
RRID:	AB_2835533

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%)

Clonality:

Polyclonal

Specificity:

CD153 Antibody detects endogenous levels of total CD153.

RRID:

AB_2835533
Cite Format: Affinity Biosciences Cat# DF3156, RRID:AB_2835533.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD153; CD153 antigen; CD30 antigen ligand; CD30 L; CD30 ligand; CD30-L; CD30L; CD30LG; MGC138144; TNFL8_HUMAN; Tnfsf8; Tumor necrosis factor (ligand) superfamily member 8; Tumor necrosis factor ligand superfamily member 8;

Immunogens

Immunogen:

Uniprot:

P32971(TNFL8_HUMAN) >>Visit HPA database.

Gene(ID):

TNFSF8(944)

Sequence:

P32971 TNFL8_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDPGLQQALNGMAPPGDTAMHVPAGSVASHLGTTSRSYFYLTTATLALCLVFTVATIMVLVVQRTDSIPNSPDNVPLKGGNCSEDLLCILKRAPFKKSWAYLQVAKHLNKTKLSWNKDGILHGVRYQDGNLVIQFPGLYFIICQLQFLVQCPNNSVDLKLELLINKHIKKQALVTVCESGMQTKHVYQNLSQFLLDYLQVNTTISVNVDTFQYIDTSTFPLENVLSIFLYSNSD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

Chicken

Pig

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P32971 As Substrate

P32971

Site	PTM Type	Enzyme	Source
S67	Phosphorylation		Uniprot
S71	Phosphorylation		Uniprot

Research Backgrounds

P32971_TNFL8_HUMAN

Function:

Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.

Subcellular Location:

Membrane>Single-pass type II membrane protein.

Subunit Structure:

Homotrimer.

Family&Domains:

Belongs to the tumor necrosis factor family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

CD153 Antibody - #DF3156