Product: TF2H1 Antibody
Catalog: DF3155
Description: Rabbit polyclonal antibody to TF2H1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 57 KD; 62kD(Calculated).
Uniprot: P32780
RRID: AB_2835532

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(91%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
TF2H1 Antibody detects endogenous levels of total TF2H1.
RRID:
AB_2835532
Cite Format: Affinity Biosciences Cat# DF3155, RRID:AB_2835532.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Basic transcription factor 2 62 kDa subunit; Basic transcription factor 62 kDa subunit; BTF 2; BTF2; BTF2 p62; General transcription factor IIH polypeptide 1; General transcription factor IIH subunit 1; General transcription factor IIH, polypeptide 1, 62kDa; gtf2h1; TF2H1; TF2H1_HUMAN; TFB1; TFIIH; TFIIH basal transcription factor complex p62 subunit;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MATSSEEVLLIVKKVRQKKQDGALYLMAERIAWAPEGKDRFTISHMYADIKCQKISPEGKAKIQLQLVLHAGDTTNFHFSNESTAVKERDAVKDLLQQLLPKFKRKANKELEEKNRMLQEDPVLFQLYKDLVVSQVISAEEFWANRLNVNATDSSSTSNHKQDVGISAAFLADVRPQTDGCNGLRYNLTSDIIESIFRTYPAVKMKYAENVPHNMTEKEFWTRFFQSHYFHRDRLNTGSKDLFAECAKIDEKGLKTMVSLGVKNPLLDLTALEDKPLDEGYGISSVPSASNSKSIKENSNAAIIKRFNHHSAMVLAAGLRKQEAQNEQTSEPSNMDGNSGDADCFQPAVKRAKLQESIEYEDLGKNNSVKTIALNLKKSDRYYHGPTPIQSLQYATSQDIINSFQSIRQEMEAYTPKLTQVLSSSAASSTITALSPGGALMQGGTQQAINQMVPNDIQSELKHLYVAVGELLRHFWSCFPVNTPFLEEKVVKMKSNLERFQVTKLCPFQEKIRRQYLSTNLVSHIEEMLQTAYNKLHTWQSRRLMKKT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
91
Chicken
91
Zebrafish
82
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P32780 As Substrate

Site PTM Type Enzyme
Y25 Phosphorylation
K38 Ubiquitination
T74 Phosphorylation
K93 Ubiquitination
K102 Ubiquitination
S158 Phosphorylation
Y207 Phosphorylation
K218 Ubiquitination
S239 Phosphorylation
K240 Acetylation
K240 Ubiquitination
K275 Ubiquitination
K293 Ubiquitination
K296 Ubiquitination
S339 Phosphorylation
K353 Ubiquitination
S357 Phosphorylation
Y360 Phosphorylation
K365 Ubiquitination
S368 Phosphorylation
K377 Ubiquitination
T415 Phosphorylation
K504 Ubiquitination

PTMs - P32780 As Enzyme

Substrate Site Source
Q01094 (E2F1) S403 Uniprot
Q01094 (E2F1) T433 Uniprot

Research Backgrounds

Function:

Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the 7-subunit TFIIH core complex composed of XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and GTF2H5, which is active in NER. The core complex associates with the 3-subunit CDK-activating kinase (CAK) module composed of CCNH/cyclin H, CDK7 and MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription. Interacts with PUF60.

Family&Domains:

Belongs to the TFB1 family.

Research Fields

· Genetic Information Processing > Transcription > Basal transcription factors.

· Genetic Information Processing > Replication and repair > Nucleotide excision repair.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

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