JAM1 Recombinant Rabbit mAb - #BF3549

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Product: JAM1 Recombinant Rabbit mAb
Catalog: BF3549
Description: Rabbit monoclonal antibody to JAM1
Application: WB IF/ICC
Reactivity: Human
Mol.Wt.: 33 kDa(Observed); 33kD(Calculated).
Uniprot: Q9Y624

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 100ul $280 In stock

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MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit IgG
Application:
WB 1:1000, IF/ICC 1:50
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [ReFirm22996]
Specificity:
JAM1 Recombinant Rabbit mAb detects endogenous levels of JAM1.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CD 321; CD321; CD321 antigen; ESTM33; F11 receptor; F11R; JAM 1; JAM A; JAM; JAM-1; JAM-A; JAM1; JAM1_HUMAN; JAMA; JCAM; Jcam1; Junction adhesion molecule 1; Junction adhesion molecule, mouse, homolog of; Junctional adhesion molecule 1; Junctional adhesion molecule A; KAT; Ly106; PAM 1; PAM-1; PAM1; Platelet adhesion molecule 1; Platelet adhesion molecule; Platelet F11 receptor; PRO301; UNQ264;

Immunogens

Immunogen:

A synthetic peptide from human JAM1

Uniprot:

Q9Y624(JAM1_HUMAN) >>Visit HPA database.

Gene(ID):
F11R(50848)
Expression:
Q9Y624 JAM1_HUMAN:

Expressed in endothelium, epithelium and leukocytes (at protein level).

Description:
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets,forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition,the encoded protein can act as (1) a receptor for reovirus,(2) a ligand for the integrin LFA1,involved in leukocyte transmigration,and (3) a platelet receptor. Multiple 5' alternatively spliced variants,encoding the same protein,have been identified but their biological validity has not been established.
Sequence:
MGTKAQVERKLLCLFILAILLCSLALGSVTVHSSEPEVRIPENNPVKLSCAYSGFSSPRVEWKFDQGDTTRLVCYNNKITASYEDRVTFLPTGITFKSVTREDTGTYTCMVSEEGGNSYGEVKVKLIVLVPPSKPTVNIPSSATIGNRAVLTCSEQDGSPPSEYTWFKDGIVMPTNPKSTRAFSNSSYVLNPTTGELVFDPLSASDTGEYSCEARNGYGTPMTSNAVRMEAVERNVGVIVAAVLVTLILLGILVFGIWFAYSRGHFDRTKKGTSSKKVIYSQPSARSEGEFKQTSSFLV

Research Backgrounds

Function:

Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier (By similarity). Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration. Involved in platelet activation.

(Microbial infection) Acts as a receptor for Mammalian reovirus sigma-1.

(Microbial infection) Acts as a receptor for Human Rotavirus strain Wa.

PTMs:

N-glycosylated.

Subcellular Location:

Cell junction>Tight junction. Cell membrane>Single-pass type I membrane protein.
Note: Localized at tight junctions of both epithelial and endothelial cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in endothelium, epithelium and leukocytes (at protein level).

Family&Domains:

The Ig-like V-type 2 domain is necessary and sufficient for interaction with integrin alpha-L/beta-2.

Belongs to the immunoglobulin superfamily.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

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