CORO1A Antibody - #DF3148

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Product: CORO1A Antibody
Catalog: DF3148
Description: Rabbit polyclonal antibody to CORO1A
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 52 KD; 51kD(Calculated).
Uniprot: P31146
RRID: AB_2835525

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
CORO1A Antibody detects endogenous levels of total CORO1A.
RRID:
AB_2835525
Cite Format: Affinity Biosciences Cat# DF3148, RRID:AB_2835525.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Actin binding protein; CLABP; Clipin A; Clipin-A; CLIPINA; COR1A_HUMAN; CORO1A; Coronin 1; Coronin actin binding protein 1A; Coronin like protein A; Coronin like protein p57; Coronin-1A; Coronin-like protein A; Coronin-like protein p57; FLJ41407; HCORO1; MGC117380; OTTHUMP00000163017; p57; TACO; Tryptophan aspartate containing coat protein; Tryptophan aspartate-containing coat protein;

Immunogens

Immunogen:
Uniprot:

P31146(COR1A_HUMAN) >>Visit HPA database.

Gene(ID):
CORO1A(11151)
Expression:
P31146 COR1A_HUMAN:

Expressed in brain, thymus, spleen, bone marrow and lymph node. Low in lung and gut.

Sequence:
MSRQVVRSSKFRHVFGQPAKADQCYEDVRVSQTTWDSGFCAVNPKFVALICEASGGGAFLVLPLGKTGRVDKNAPTVCGHTAPVLDIAWCPHNDNVIASGSEDCTVMVWEIPDGGLMLPLREPVVTLEGHTKRVGIVAWHTTAQNVLLSAGCDNVIMVWDVGTGAAMLTLGPEVHPDTIYSVDWSRDGGLICTSCRDKRVRIIEPRKGTVVAEKDRPHEGTRPVRAVFVSEGKILTTGFSRMSERQVALWDTKHLEEPLSLQELDTSSGVLLPFFDPDTNIVYLCGKGDSSIRYFEITSEAPFLHYLSMFSSKESQRGMGYMPKRGLEVNKCEIARFYKLHERRCEPIAMTVPRKSDLFQEDLYPPTAGPDPALTAEEWLGGRDAGPLLISLKDGYVPPKSRELRVNRGLDTGRRRAAPEASGTPSSDAVSRLEEEMRKLQATVQELQKRLDRLEETVQAK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
88
Xenopus
75
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P31146 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
K20 Acetylation
K20 Ubiquitination
Y25 Phosphorylation
T33 Phosphorylation
T34 Phosphorylation
S54 Phosphorylation
K66 Ubiquitination
T178 Phosphorylation
Y180 Phosphorylation
S181 Phosphorylation
K207 Ubiquitination
K214 Ubiquitination
K233 Acetylation
K233 Ubiquitination
K253 Ubiquitination
S299 Phosphorylation
K313 Acetylation
K313 Ubiquitination
K324 Ubiquitination
K331 Acetylation
K331 Ubiquitination
K339 Acetylation
K339 Ubiquitination
K355 Ubiquitination
S356 Phosphorylation
Y364 Phosphorylation
K393 Ubiquitination
K400 Ubiquitination
T412 Phosphorylation
S422 Phosphorylation
T424 Phosphorylation Q00535 (CDK5)
S426 Phosphorylation
S431 Phosphorylation
K439 Ubiquitination
K449 Acetylation
K449 Ubiquitination

Research Backgrounds

Function:

May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria-infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes.

PTMs:

phosphorylation at Thr-412 by PKC strongly down-regulates the association with actin.

Polyubiquitinated by RNF128 with 'Lys-48'-linked chains, leading to proteasomal degradation.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cytoplasm>Cell cortex. Cytoplasmic vesicle>Phagosome membrane.
Note: In non-infected macrophages, associated with the cortical microtubule network. In mycobacteria-infected macrophages, becomes progressively relocalized and retained around the mycobacterial phagosomes. Retention on the phagosomal membrane is strictly dependent on mycobacterial viability and not due to impaired acidification (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in brain, thymus, spleen, bone marrow and lymph node. Low in lung and gut.

Subunit Structure:

Binds actin.

Family&Domains:

Belongs to the WD repeat coronin family.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

References

1). Wnt5a regulates Ameloblastoma Cell Migration by modulating Mitochondrial and Cytoskeletal Dynamics. Journal of Cancer, 2020 (PubMed: 32742496) [IF=3.9]

Application: WB    Species: human    Sample: AM-1 cells

Figure 5. |Wnt5a up-regulates mitochondria-cytoskeleton co-localization through mediating Coro1A expression. (A) Western blot detected nuclear transfactor NFATc2 and Coro1A protein expression. Bar charts demonstrating quantitative significance results from 3 to 5 independent experiments. (B) Western blot demonstrating Coro1A protein expression changes of AM-1 cells treated with only human recombinant Wnt5a compared with cells in the presence of both rhWnt5a and FK506 (200 nm).

Application: IHC    Species: human    Sample: oral mucosa tissues and ameloblastoma tissues

Figure 5. |Wnt5a up-regulates mitochondria-cytoskeleton co-localization through mediating Coro1A expression. (E) Immunohistochemistry staining demonstrated Coro1A protein expression in normal oral mucosa tissues (a), (b-d) ameloblastoma tissues (forlicular type for b, plexiform type for c, peripheral type for d). Magnification: 200×. *p<0.05.

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