TF2E2 Antibody - #DF3144

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Product: TF2E2 Antibody
Catalog: DF3144
Description: Rabbit polyclonal antibody to TF2E2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 33 KD; 33kD(Calculated).
Uniprot: P29084
RRID: AB_2835521

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(91%), Chicken(91%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
TF2E2 Antibody detects endogenous levels of total TF2E2.
RRID:
AB_2835521
Cite Format: Affinity Biosciences Cat# DF3144, RRID:AB_2835521.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FE; General transcription factor IIE subunit 2; General transcription factor IIE, polypeptide 2, beta 34kDa; gtf2e2; T2EB_HUMAN; TF2E 2; TF2E2; TFIIE B; TFIIE-beta; Transcription initiation factor IIE beta subunit; Transcription initiation factor IIE subunit beta;

Immunogens

Immunogen:
Uniprot:

P29084(T2EB_HUMAN) >>Visit HPA database.

Gene(ID):
GTF2E2(2961)
Sequence:
MDPSLLRERELFKKRALSTPVVEKRSASSESSSSSSKKKKTKVEHGGSSGSKQNSDHSNGSFNLKALSGSSGYKFGVLAKIVNYMKTRHQRGDTHPLTLDEILDETQHLDIGLKQKQWLMTEALVNNPKIEVIDGKYAFKPKYNVRDKKALLRLLDQHDQRGLGGILLEDIEEALPNSQKAVKALGDQILFVNRPDKKKILFFNDKSCQFSVDEEFQKLWRSVTVDSMDEEKIEEYLKRQGISSMQESGPKKVAPIQRRKKPASQKKRRFKTHNEHLAGVLKDYSDITSSK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Dog
91
Chicken
91
Xenopus
82
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P29084 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S4 Phosphorylation
S18 Phosphorylation
T19 Phosphorylation
K24 Acetylation
S26 Phosphorylation
S29 Phosphorylation
S35 Phosphorylation
S36 Phosphorylation
K52 Acetylation
S58 Phosphorylation
S61 Phosphorylation
K86 Ubiquitination
T87 Phosphorylation
K114 Ubiquitination
K116 Acetylation
T121 Phosphorylation
K129 Acetylation
K180 Ubiquitination
K183 Ubiquitination
K198 Acetylation
K206 Acetylation
K218 Ubiquitination
S227 Phosphorylation
K238 Methylation
K238 Ubiquitination
S264 Phosphorylation
K271 Ubiquitination
K282 Ubiquitination
Y284 Phosphorylation

Research Backgrounds

Function:

Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Tetramer of two alpha and two beta chains. Interacts with FACT subunit SUPT16H. Interacts with ATF7IP. Interacts with SND1.

Family&Domains:

Belongs to the TFIIE beta subunit family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.