TF2E2 Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using TF2E2 Antibody.

Product:	TF2E2 Antibody
Catalog:	DF3144
Description:	Rabbit polyclonal antibody to TF2E2
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	33 KD; 33kD(Calculated).
Uniprot:	P29084
RRID:	AB_2835521

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(91%), Chicken(91%), Xenopus(82%)

Clonality:

Polyclonal

Specificity:

TF2E2 Antibody detects endogenous levels of total TF2E2.

RRID:

AB_2835521
Cite Format: Affinity Biosciences Cat# DF3144, RRID:AB_2835521.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FE; General transcription factor IIE subunit 2; General transcription factor IIE, polypeptide 2, beta 34kDa; gtf2e2; T2EB_HUMAN; TF2E 2; TF2E2; TFIIE B; TFIIE-beta; Transcription initiation factor IIE beta subunit; Transcription initiation factor IIE subunit beta;

Immunogens

Immunogen:

Uniprot:

P29084(T2EB_HUMAN) >>Visit HPA database.

Gene(ID):

GTF2E2(2961)

Sequence:

P29084 T2EB_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDPSLLRERELFKKRALSTPVVEKRSASSESSSSSSKKKKTKVEHGGSSGSKQNSDHSNGSFNLKALSGSSGYKFGVLAKIVNYMKTRHQRGDTHPLTLDEILDETQHLDIGLKQKQWLMTEALVNNPKIEVIDGKYAFKPKYNVRDKKALLRLLDQHDQRGLGGILLEDIEEALPNSQKAVKALGDQILFVNRPDKKKILFFNDKSCQFSVDEEFQKLWRSVTVDSMDEEKIEEYLKRQGISSMQESGPKKVAPIQRRKKPASQKKRRFKTHNEHLAGVLKDYSDITSSK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Rabbit

100

Dog

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P29084 As Substrate

P29084

Site	PTM Type	Source
M1	Acetylation	Uniprot
S4	Phosphorylation	Uniprot
S18	Phosphorylation	Uniprot
T19	Phosphorylation	Uniprot
K24	Acetylation	Uniprot
S26	Phosphorylation	Uniprot
S29	Phosphorylation	Uniprot
S35	Phosphorylation	Uniprot
S36	Phosphorylation	Uniprot
K52	Acetylation	Uniprot
S58	Phosphorylation	Uniprot
S61	Phosphorylation	Uniprot
K86	Ubiquitination	Uniprot
T87	Phosphorylation	Uniprot
K114	Ubiquitination	Uniprot
K116	Acetylation	Uniprot
T121	Phosphorylation	Uniprot
K129	Acetylation	Uniprot
K180	Ubiquitination	Uniprot
K183	Ubiquitination	Uniprot
K198	Acetylation	Uniprot
K206	Acetylation	Uniprot
K218	Ubiquitination	Uniprot
S227	Phosphorylation	Uniprot
K238	Methylation	Uniprot
K238	Ubiquitination	Uniprot
S264	Phosphorylation	Uniprot
K271	Ubiquitination	Uniprot
K282	Ubiquitination	Uniprot
Y284	Phosphorylation	Uniprot

Research Backgrounds

P29084_T2EB_HUMAN

Function:

Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.

Subcellular Location:

Nucleus.

Subunit Structure:

Tetramer of two alpha and two beta chains. Interacts with FACT subunit SUPT16H. Interacts with ATF7IP. Interacts with SND1.

Family&Domains:

Belongs to the TFIIE beta subunit family.

Research Fields

· Genetic Information Processing > Transcription > Basal transcription factors.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

TF2E2 Antibody - #DF3144