RPL10 Antibody - #DF3137

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Product: RPL10 Antibody
Catalog: DF3137
Description: Rabbit polyclonal antibody to RPL10
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.: 25 KD; 25kD(Calculated).
Uniprot: P27635
RRID: AB_2835514

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 100ul $280 In stock
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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
RPL10 Antibody detects endogenous levels of total RPL10.
RRID:
AB_2835514
Cite Format: Affinity Biosciences Cat# DF3137, RRID:AB_2835514.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

60S ribosomal protein L10; DKFZp686J1851; DXS648; DXS648E; FLJ23544; FLJ27072; L10; Laminin receptor homolog; NOV; OTTHUMP00000031945; OTTHUMP00000063212; OTTHUMP00000063213; OTTHUMP00000183506; OTTHUMP00000196447; Protein QM; QM; Ribosomal protein L10; Tumor suppressor QM; Wilms tumor related protein;

Immunogens

Immunogen:
Uniprot:

P27635(RL10_HUMAN) >>Visit HPA database.

Gene(ID):
RPL10(6134)
Sequence:
MGRRPARCYRYCKNKPYPKSRFCRGVPDAKIRIFDLGRKKAKVDEFPLCGHMVSDEYEQLSSEALEAARICANKYMVKSCGKDGFHIRVRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVHIGQVIMSIRTKLQNKEHVIEALRRAKFKFPGRQKIHISKKWGFTKFNADEFEDMVAEKRLIPDGCGVKYIPNRGPLDKWRALHS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P27635 As Substrate

Site PTM Type Enzyme
Y9 Phosphorylation
K13 Ubiquitination
Y17 Phosphorylation
K19 Ubiquitination
R24 Methylation
K30 Sumoylation
K30 Ubiquitination
R32 Methylation
K42 Ubiquitination
S54 Phosphorylation
Y57 Phosphorylation
S61 Phosphorylation
C71 S-Nitrosylation
K74 Acetylation
K74 Sumoylation
K74 Ubiquitination
Y75 Phosphorylation
K78 Ubiquitination
K82 Sumoylation
K82 Ubiquitination
R88 Methylation
K101 Ubiquitination
S104 Phosphorylation
R110 Methylation
K121 Acetylation
K121 Sumoylation
K121 Ubiquitination
T125 Phosphorylation
K145 Ubiquitination
R153 Methylation
K156 Ubiquitination
K158 Sumoylation
K158 Ubiquitination
K164 Acetylation
K164 Ubiquitination
K170 Methylation
K170 Sumoylation
K170 Ubiquitination
K175 Sumoylation
K175 Ubiquitination
K188 Sumoylation
K188 Ubiquitination
C195 S-Nitrosylation
K198 Ubiquitination
K208 Acetylation
K208 Ubiquitination

Research Backgrounds

Function:

Component of the large ribosomal subunit. Plays a role in the formation of actively translating ribosomes. May play a role in the embryonic brain development.

PTMs:

Citrullinated by PADI4.

Subunit Structure:

Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (28S, 5.8S and 5S).

Family&Domains:

Belongs to the universal ribosomal protein uL16 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

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