CDC2L1 Antibody - #DF3118

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Product: CDC2L1 Antibody
Catalog: DF3118
Description: Rabbit polyclonal antibody to CDC2L1
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Xenopus
Mol.Wt.: 92 KD; 93kD(Calculated).
Uniprot: P21127
RRID: AB_2835495

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
CDC2L1 Antibody detects endogenous levels of total CDC2L1.
RRID:
AB_2835495
Cite Format: Affinity Biosciences Cat# DF3118, RRID:AB_2835495.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CDC2L1; CDK11; Cell division cycle 2-like protein kinase 1; Cell division protein kinase 11B; CLK 1; EC=2.7.11.22; Galactosyltransferase associated protein kinase p58/GTA; ITSLRE serine/threonine protein kinase CDC2L1; p58 CLK 1; PITSLREA; PK58;

Immunogens

Immunogen:
Uniprot:

P21127(CD11B_HUMAN) >>Visit HPA database.

Gene(ID):
CDK11B(984)
Expression:
P21127 CD11B_HUMAN:

Expressed ubiquitously. Some evidence of isoform-specific tissue distribution.

Sequence:
MGDEKDSWKVKTLDEILQEKKRRKEQEEKAEIKRLKNSDDRDSKRDSLEEGELRDHRMEITIRNSPYRREDSMEDRGEEDDSLAIKPPQQMSRKEKAHHRKDEKRKEKRRHRSHSAEGGKHARVKEKEREHERRKRHREEQDKARREWERQKRREMAREHSRRERDRLEQLERKRERERKMREQQKEQREQKERERRAEERRKEREARREVSAHHRTMREDYSDKVKASHWSRSPPRPPRERFELGDGRKPGEARPAPAQKPAQLKEEKMEERDLLSDLQDISDSERKTSSAESSSAESGSGSEEEEEEEEEEEEEGSTSEESEEEEEEEEEEEEETGSNSEEASEQSAEEVSEEEMSEDEERENENHLLVVPESRFDRDSGESEEAEEEVGEGTPQSSALTEGDYVPDSPALSPIELKQELPKYLPALQGCRSVEEFQCLNRIEEGTYGVVYRAKDKKTDEIVALKRLKMEKEKEGFPITSLREINTILKAQHPNIVTVREIVVGSNMDKIYIVMNYVEHDLKSLMETMKQPFLPGEVKTLMIQLLRGVKHLHDNWILHRDLKTSNLLLSHAGILKVGDFGLAREYGSPLKAYTPVVVTLWYRAPELLLGAKEYSTAVDMWSVGCIFGELLTQKPLFPGKSEIDQINKVFKDLGTPSEKIWPGYSELPAVKKMTFSEHPYNNLRKRFGALLSDQGFDLMNKFLTYFPGRRISAEDGLKHEYFRETPLPIDPSMFPTWPAKSEQQRVKRGTSPRPPEGGLGYSQLGDDDLKETGFHLTTTNQGASAAGPGFSLKF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Xenopus
82
Zebrafish
64
Horse
0
Sheep
0
Dog
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P21127 As Substrate

Site PTM Type Enzyme
K11 Sumoylation
K11 Ubiquitination
K20 Sumoylation
K20 Ubiquitination
K29 Sumoylation
S43 Phosphorylation
S47 Phosphorylation
T61 Phosphorylation
S65 Phosphorylation
Y67 Phosphorylation
S72 Phosphorylation
K101 Acetylation
S113 Phosphorylation
S115 Phosphorylation
K135 Acetylation
S212 Phosphorylation
Y222 Phosphorylation
S223 Phosphorylation
K225 Acetylation
S229 Phosphorylation
S232 Phosphorylation
S234 Phosphorylation
K266 Sumoylation
S277 Phosphorylation
S283 Phosphorylation
S285 Phosphorylation
S358 Phosphorylation
S381 Phosphorylation
S384 Phosphorylation
T395 Phosphorylation
T402 Phosphorylation
Y406 Phosphorylation
S410 Phosphorylation
S414 Phosphorylation
K419 Sumoylation
K424 Ubiquitination
S434 Phosphorylation
T448 Phosphorylation
Y449 Phosphorylation
Y453 Phosphorylation
K456 Acetylation
K458 Acetylation
K459 Acetylation
K459 Sumoylation
K459 Ubiquitination
K467 Acetylation
K467 Sumoylation
K467 Ubiquitination
K475 Ubiquitination
S482 Phosphorylation
T488 Phosphorylation
K491 Sumoylation
K491 Ubiquitination
K531 Ubiquitination
K551 Ubiquitination
K564 Sumoylation
K564 Ubiquitination
K577 Ubiquitination
Y587 Phosphorylation
S589 Phosphorylation
Y594 Phosphorylation
T595 Phosphorylation P50613 (CDK7)
T600 Phosphorylation
K641 Sumoylation
K641 Ubiquitination
K649 Sumoylation
K649 Ubiquitination
K652 Sumoylation
K652 Ubiquitination
K660 Ubiquitination
K672 Ubiquitination
K673 Sumoylation
K673 Ubiquitination
T675 Phosphorylation
S713 Phosphorylation
K719 Sumoylation
K719 Ubiquitination
T726 Phosphorylation Q9UQ88 (CDK11A)
K741 Sumoylation
K741 Ubiquitination
T751 Phosphorylation
S752 Phosphorylation O96017 (CHEK2)
Y762 Phosphorylation
S763 Phosphorylation
T773 Phosphorylation
T778 Phosphorylation
S785 Phosphorylation
S792 Phosphorylation
K794 Methylation
K794 Sumoylation
K794 Ubiquitination

PTMs - P21127 As Enzyme

Substrate Site Source
O00303 (EIF3F) S46 Uniprot
O00303 (EIF3F) T119 Uniprot

Research Backgrounds

Function:

Plays multiple roles in cell cycle progression, cytokinesis and apoptosis. Involved in pre-mRNA splicing in a kinase activity-dependent manner. Isoform 7 may act as a negative regulator of normal cell cycle progression.

PTMs:

During FAS- or TNF-induced apoptosis, isoform SV9 is cleaved by caspases to produce p110C, a fragment that contains the C-terminal kinase domain.

Phosphorylation at Ser-115 creates a binding site for 14-3-3 proteins. p110C can be autophosphorylated.

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed ubiquitously. Some evidence of isoform-specific tissue distribution.

Subunit Structure:

Cleaved isoform SV9 (p110C) binds to the serine/threonine kinase PAK1 and RANBP9. p110C interacts with RNPS1. Isoform 7, but not isoform SV9, nor its cleavage product p110C, interacts with CCND3. Interacts with CCNL1 and CCNL2. Forms complexes with pre-mRNA-splicing factors, including at least SRSF1, SRSF2 AND SRSF7/SLU7. Interacts with isoform 5 of MYO18A.

Family&Domains:

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

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