CKMT2 Antibody - #DF3104

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Product: CKMT2 Antibody
Catalog: DF3104
Description: Rabbit polyclonal antibody to CKMT2
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 48 KD; 48kD(Calculated).
Uniprot: P17540
RRID: AB_2835481

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
CKMT2 Antibody detects endogenous levels of total CKMT2.
RRID:
AB_2835481
Cite Format: Affinity Biosciences Cat# DF3104, RRID:AB_2835481.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CKMT 2; Basic-type mitochondrial creatine kinase; CKMT2; CPK; Creatine kinase mitochondrial 2; Creatine kinase mitochondrial 2 (sarcomeric); Creatine kinase S-type; creatine kinase S-type, mitochondrial; Creatine kinase, sarcomeric mitochondrial; KCRS_HUMAN; Mib CK; Mib-CK; mitochondrial; OTTHUMP00000147542; S-MtCK; Sarcomeric mitochondrial creatine kinase; SMTCK;

Immunogens

Immunogen:
Uniprot:

P17540(KCRS_HUMAN) >>Visit HPA database.

Gene(ID):
CKMT2(1160)
Expression:
P17540 KCRS_HUMAN:

Sarcomere-specific. Found only in heart and skeletal muscles.

Sequence:
MASIFSKLLTGRNASLLFATMGTSVLTTGYLLNRQKVCAEVREQPRLFPPSADYPDLRKHNNCMAECLTPAIYAKLRNKVTPNGYTLDQCIQTGVDNPGHPFIKTVGMVAGDEESYEVFADLFDPVIKLRHNGYDPRVMKHTTDLDASKITQGQFDEHYVLSSRVRTGRSIRGLSLPPACTRAERREVENVAITALEGLKGDLAGRYYKLSEMTEQDQQRLIDDHFLFDKPVSPLLTCAGMARDWPDARGIWHNYDKTFLIWINEEDHTRVISMEKGGNMKRVFERFCRGLKEVERLIQERGWEFMWNERLGYILTCPSNLGTGLRAGVHVRIPKLSKDPRFSKILENLRLQKRGTGGVDTAAVADVYDISNIDRIGRSEVELVQIVIDGVNYLVDCEKKLERGQDIKVPPPLPQFGKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P17540 As Substrate

Site PTM Type Enzyme
Y73 Phosphorylation
Y85 Phosphorylation
T142 Phosphorylation
T143 Phosphorylation
S148 Phosphorylation
T151 Phosphorylation
Y159 Phosphorylation
S162 Phosphorylation
S163 Phosphorylation
T167 Phosphorylation
S170 Phosphorylation
S175 Phosphorylation
Y207 Phosphorylation
Y208 Phosphorylation
S211 Phosphorylation
T214 Phosphorylation
Y255 Phosphorylation
K281 Acetylation
Y313 Phosphorylation
S319 Phosphorylation
S343 Phosphorylation
R350 Methylation
T356 Phosphorylation
T361 Phosphorylation
Y368 Phosphorylation
S371 Phosphorylation
Y393 Phosphorylation
K419 Acetylation

Research Backgrounds

Function:

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Subcellular Location:

Mitochondrion inner membrane>Peripheral membrane protein>Intermembrane side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Sarcomere-specific. Found only in heart and skeletal muscles.

Subunit Structure:

Exists as an octamer composed of four CKMT2 homodimers.

Family&Domains:

Belongs to the ATP:guanido phosphotransferase family.

Research Fields

· Metabolism > Amino acid metabolism > Arginine and proline metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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