TRIM24 Antibody - #AF0320

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Product: TRIM24 Antibody
Catalog: AF0320
Description: Rabbit polyclonal antibody to TRIM24
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 117kDa; 117kD(Calculated).
Uniprot: O15164
RRID: AB_2833483

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
TRIM24 Antibody detects endogenous levels of total TRIM24.
RRID:
AB_2833483
Cite Format: Affinity Biosciences Cat# AF0320, RRID:AB_2833483.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

E3 ubiquitin protein ligase TRIM24; E3 ubiquitin-protein ligase Trim24; hTIF1; PTC6; RING finger protein 82; RNF82; TF1A; TIF1; TIF1-alpha; TIF1A; TIF1A_HUMAN; TIF1ALPHA; Transcription intermediary factor 1-alpha; Transcriptional intermediary factor 1 alpha; Transcriptional intermediary factor 1; Trim24; Tripartite motif containing 24; Tripartite motif-containing protein 24;

Immunogens

Immunogen:
Uniprot:

O15164(TIF1A_HUMAN) >>Visit HPA database.

Gene(ID):
TRIM24(8805)
Description:
TRIM24 an atypical protein kinase that functions as a transcriptional coactivator. Originally identified as a mammalian protein that enhances the AF-2 activity of the retinoid X receptor (RXR) in a yeast genetic screen. Binds to nuclear receptors in an agonist and AF2-activating domain (AF2-AD) dependent manner. Binding to the agonist-nuclear receptor complex induces its own hyperphosphorylation as well as the phosphorylation of TFIIEa, TAFII28, and TAFII55.
Sequence:
MEVAVEKAVAAAAAASAAASGGPSAAPSGENEAESRQGPDSERGGEAARLNLLDTCAVCHQNIQSRAPKLLPCLHSFCQRCLPAPQRYLMLPAPMLGSAETPPPVPAPGSPVSGSSPFATQVGVIRCPVCSQECAERHIIDNFFVKDTTEVPSSTVEKSNQVCTSCEDNAEANGFCVECVEWLCKTCIRAHQRVKFTKDHTVRQKEEVSPEAVGVTSQRPVFCPFHKKEQLKLYCETCDKLTCRDCQLLEHKEHRYQFIEEAFQNQKVIIDTLITKLMEKTKYIKFTGNQIQNRIIEVNQNQKQVEQDIKVAIFTLMVEINKKGKALLHQLESLAKDHRMKLMQQQQEVAGLSKQLEHVMHFSKWAVSSGSSTALLYSKRLITYRLRHLLRARCDASPVTNNTIQFHCDPSFWAQNIINLGSLVIEDKESQPQMPKQNPVVEQNSQPPSGLSSNQLSKFPTQISLAQLRLQHMQQQVMAQRQQVQRRPAPVGLPNPRMQGPIQQPSISHQQPPPRLINFQNHSPKPNGPVLPPHPQQLRYPPNQNIPRQAIKPNPLQMAFLAQQAIKQWQISSGQGTPSTTNSTSSTPSSPTITSAAGYDGKAFGSPMIDLSSPVGGSYNLPSLPDIDCSSTIMLDNIVRKDTNIDHGQPRPPSNRTVQSPNSSVPSPGLAGPVTMTSVHPPIRSPSASSVGSRGSSGSSSKPAGADSTHKVPVVMLEPIRIKQENSGPPENYDFPVVIVKQESDEESRPQNANYPRSILTSLLLNSSQSSTSEETVLRSDAPDSTGDQPGLHQDNSSNGKSEWLDPSQKSPLHVGETRKEDDPNEDWCAVCQNGGELLCCEKCPKVFHLSCHVPTLTNFPSGEWICTFCRDLSKPEVEYDCDAPSHNSEKKKTEGLVKLTPIDKRKCERLLLFLYCHEMSLAFQDPVPLTVPDYYKIIKNPMDLSTIKKRLQEDYSMYSKPEDFVADFRLIFQNCAEFNEPDSEVANAGIKLENYFEELLKNLYPEKRFPKPEFRNESEDNKFSDDSDDDFVQPRKKRLKSIEERQLLK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15164 As Substrate

Site PTM Type Enzyme
S41 Phosphorylation
T101 Phosphorylation
S110 Phosphorylation
T148 Phosphorylation
S153 Phosphorylation
S154 Phosphorylation
S209 Phosphorylation
Y234 Phosphorylation
K252 Ubiquitination
K285 Ubiquitination
K303 Ubiquitination
K325 Ubiquitination
K336 Ubiquitination
K341 Ubiquitination
S452 Phosphorylation
K458 Ubiquitination
R469 Methylation
R481 Methylation
S590 Phosphorylation
S606 Phosphorylation
K641 Ubiquitination
S654 Phosphorylation
T657 Phosphorylation
S660 Phosphorylation
S663 Phosphorylation
S664 Phosphorylation
S667 Phosphorylation
S685 Phosphorylation
S687 Phosphorylation
S696 Phosphorylation
K702 Sumoylation
K711 Sumoylation
K723 Sumoylation
Y733 Phosphorylation
K741 Sumoylation
S744 Phosphorylation
S748 Phosphorylation
S762 Phosphorylation
S768 Phosphorylation
S771 Phosphorylation
T772 Phosphorylation
S773 Phosphorylation
T776 Phosphorylation
T786 Phosphorylation
S797 Phosphorylation
K801 Sumoylation
S808 Phosphorylation
S811 Phosphorylation
T818 Phosphorylation
S874 Phosphorylation
Y916 Phosphorylation
Y936 Phosphorylation
K940 Sumoylation
K949 Sumoylation
K992 Sumoylation
Y996 Phosphorylation
K1002 Ubiquitination
S1019 Phosphorylation
S1025 Phosphorylation
S1028 Phosphorylation
S1042 Phosphorylation

Research Backgrounds

Function:

Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes (By similarity).

PTMs:

Sumoylated.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Colocalizes with sites of active transcription. Detected both in nucleus and cytoplasm in some breast cancer samples. Predominantly nuclear.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with CARM1, NCOA2/GRIP1, PML, KAT5/TIP60, BRD7, CBX1, CBX3 and CBX5. Part of a coactivator complex containing TRIM24, NCOA2 and CARM1 (By similarity). Interacts with NR3C2/MCR. Interacts with the ligand-binding domain of estrogen receptors (in vitro). Interaction with DNA-bound estrogen receptors requires the presence of estradiol. Interacts with AR and p53/TP53. Interacts (via bromo domain) with histone H3 (via N-terminus), provided that it is not methylated at 'Lys-4' (H3K4me0). Does not interact with histone H3 that is methylated at 'Lys-4' (H3K4me1, H3K4me2 or H3K4me3). Interacts (via bromo domain) with histone H3 (via N-terminus) that is acetylated at 'Lys-23' (H3K23ac). Has the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has very low affinity for histone H3 that is methylated at 'Lys-9' (H3K9me), or acetylated at both 'Lys-9' (H3K9ac) and 'Lys-14' (H3K14ac), or acetylated at 'Lys-27' (H3K27ac) (in vitro). Interacts with TRIM16.

References

1). E3 ubiquitin ligase TRIM24 deficiency promotes NLRP3/Caspase‐1/IL‐1β‐mediated pyroptosis in endometriosis. CELL BIOLOGY INTERNATIONAL, 2021 (PubMed: 33724611) [IF=3.9]

Application: WB    Species: Human    Sample: ectopic endometrial tissues

FIGURE 1 The expression of tripartite motif‐containing 24 (TRIM24) was decreased in ectopic endometrial tissues. (a) The expression of TRIM24 in the normal endometrium (NE) and ectopic endometrium of endometriosis (EMS) was detected by immunohistochemistry (IHC). Representative IHC images are shown. Scale bar=50μm; (b) TRIM24 protein expression was measured by Western blot analysis in NE and EMS. The data are expressed as means±SD. GAPDH, glyceraldehyde 3‐phosphate dehydrogenase. *p<.05 by two‐tailed Student's t test

Application: IHC    Species: Human    Sample: ectopic endometrial tissues

FIGURE 3 TRIM24 negatively regulated pyroptosis and cell migration of hESC. (a, b) The transfection efficiency of TRIM24 using TRIM24‐small‐interfering RNA (siTRIM24) or TRIM24‐pcDNA3.1 (TRIM24 over ) was detected by real‐time polymerase chain reaction (a) and Western blot analysis (b); (c) representative Western blots of TRIM24 and pyroptotic proteins; (d, e) the quantitative comparison of protein expression; (f, g) the supernatant concentrations of IL‐1β and IL‐18 were tested by enzyme‐linked immunosorbent assay; (h) after being transfected with siTRIM24, the migration of normal hESC was measured by Transwell migration assay; (i) after being transfected with TRIM24‐ pcDNA3.1 (TRIM24 over ), the migration of ectopic hESC was detected by Transwell migration assay. Scale bar=100 μm. NC represents negative control groups. The data are expressed as means±SD. GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; hESC, human endometrial stromal cell; IL, interleukin; TRIM24, tripartite motif‐containing 24. **p<.01 and ***p<.001 by one‐way analysis of variance with Bonferroni's multiple comparison tests

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