HARS Antibody - #DF3087

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Product: HARS Antibody
Catalog: DF3087
Description: Rabbit polyclonal antibody to HARS
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 60/52 KD; 57kD(Calculated).
Uniprot: P12081
RRID: AB_2835470

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(80%)
Clonality:
Polyclonal
Specificity:
HARS Antibody detects endogenous levels of total HARS.
RRID:
AB_2835470
Cite Format: Affinity Biosciences Cat# DF3087, RRID:AB_2835470.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

cytoplasmic; EC 6.1.1.21; FLJ20491; HARS; HisRS; Histidine tRNA ligase, cytoplasmic; histidine translase; Histidine tRNA ligase; Histidine--tRNA ligase; Histidyl tRNA synthetase; Histidyl-tRNA synthetase; HRS; Human histidyl tRNA synthetase homolog (HO3) mRNA complete cds; SYHC_HUMAN; USH3B;

Immunogens

Immunogen:
Uniprot:

P12081(HARS1_HUMAN) >>Visit HPA database.

Gene(ID):
HARS(3035)
Expression:
P12081 HARS1_HUMAN:

Brain, heart, liver and kidney.

Sequence:
MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKVFDVIIRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMIPDAECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKLLFEYLTLFGIDDKISFDLSLARGLDYYTGVIYEAVLLQTPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEALEEKIRTTETQVLVASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAGIPLVAIIGEQELKDGVIKLRSVTSREEVDVRREDLVEEIKRRTGQPLCIC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
80
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P12081 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K12 Ubiquitination
S27 Phosphorylation
K37 Ubiquitination
K42 Ubiquitination
K51 Ubiquitination
K57 Methylation
K57 Ubiquitination
T58 Phosphorylation
S66 Phosphorylation
K75 Ubiquitination
T94 Phosphorylation
K100 Ubiquitination
K106 Ubiquitination
K112 Acetylation
K112 Ubiquitination
Y115 Phosphorylation
K118 Acetylation
K118 Ubiquitination
S126 Phosphorylation
Y138 Phosphorylation
Y150 Phosphorylation
Y156 Phosphorylation
S229 Phosphorylation
K230 Ubiquitination
K240 Ubiquitination
K243 Ubiquitination
K250 Ubiquitination
K257 Ubiquitination
Y270 Phosphorylation
K288 Ubiquitination
K293 Ubiquitination
S356 Phosphorylation
Y363 Phosphorylation
K373 Ubiquitination
K376 Ubiquitination
S391 Phosphorylation
K403 Ubiquitination
S415 Phosphorylation
K418 Ubiquitination
K426 Ubiquitination
K443 Acetylation
K477 Ubiquitination
K499 Ubiquitination

Research Backgrounds

Function:

Catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP). Plays a role in axon guidance.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Brain, heart, liver and kidney.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the class-II aminoacyl-tRNA synthetase family.

Research Fields

· Genetic Information Processing > Translation > Aminoacyl-tRNA biosynthesis.

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