Product: DNA Polymerase alpha Antibody
Catalog: DF3082
Description: Rabbit polyclonal antibody to DNA Polymerase alpha
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Horse
Mol.Wt.: 165 KD; 166kD(Calculated).
Uniprot: P09884
RRID: AB_2835465

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Horse(100%)
Clonality:
Polyclonal
Specificity:
DNA Polymerase alpha Antibody detects endogenous levels of total DNA Polymerase alpha.
RRID:
AB_2835465
Cite Format: Affinity Biosciences Cat# DF3082, RRID:AB_2835465.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp686K1672; DNA polymerase alpha 1 catalytic subunit; DNA polymerase alpha catalytic subunit; DNA polymerase alpha catalytic subunit p180; DNA polymerase alpha p180 subunit; DPOLA_HUMAN; OTTHUMP00000023089; OTTHUMP00000023090; P180; p180 subunit; POLA; POLA1; Polymerase (DNA directed), alpha 1, catalytic subunit; Polymerase (DNA-directed), alpha (70kD);

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MAPVHGDDSLSDSGSFVSSRARREKKSKKGRQEALERLKKAKAGEKYKYEVEDFTGVYEEVDEEQYSKLVQARQDDDWIVDDDGIGYVEDGREIFDDDLEDDALDADEKGKDGKARNKDKRNVKKLAVTKPNNIKSMFIACAGKKTADKAVDLSKDGLLGDILQDLNTETPQITPPPVMILKKKRSIGASPNPFSVHTATAVPSGKIASPVSRKEPPLTPVPLKRAEFAGDDVQVESTEEEQESGAMEFEDGDFDEPMEVEEVDLEPMAAKAWDKESEPAEEVKQEADSGKGTVSYLGSFLPDVSCWDIDQEGDSSFSVQEVQVDSSHLPLVKGADEEQVFHFYWLDAYEDQYNQPGVVFLFGKVWIESAETHVSCCVMVKNIERTLYFLPREMKIDLNTGKETGTPISMKDVYEEFDEKIATKYKIMKFKSKPVEKNYAFEIPDVPEKSEYLEVKYSAEMPQLPQDLKGETFSHVFGTNTSSLELFLMNRKIKGPCWLEVKSPQLLNQPVSWCKVEAMALKPDLVNVIKDVSPPPLVVMAFSMKTMQNAKNHQNEIIAMAALVHHSFALDKAAPKPPFQSHFCVVSKPKDCIFPYAFKEVIEKKNVKVEVAATERTLLGFFLAKVHKIDPDIIVGHNIYGFELEVLLQRINVCKAPHWSKIGRLKRSNMPKLGGRSGFGERNATCGRMICDVEISAKELIRCKSYHLSELVQQILKTERVVIPMENIQNMYSESSQLLYLLEHTWKDAKFILQIMCELNVLPLALQITNIAGNIMSRTLMGGRSERNEFLLLHAFYENNYIVPDKQIFRKPQQKLGDEDEEIDGDTNKYKKGRKKAAYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVASEAQKVTEDGEQEQIPELPDPSLEMGILPREIRKLVERRKQVKQLMKQQDLNPDLILQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTYKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNKLYKLLEIDIDGVFKSLLLLKKKKYAALVVEPTSDGNYVTKQELKGLDIVRRDWCDLAKDTGNFVIGQILSDQSRDTIVENIQKRLIEIGENVLNGSVPVSQFEINKALTKDPQDYPDKKSLPHVHVALWINSQGGRKVKAGDTVSYVICQDGSNLTASQRAYAPEQLQKQDNLTIDTQYYLAQQIHPVVARICEPIDGIDAVLIATWLGLDPTQFRVHHYHKDEENDALLGGPAQLTDEEKYRDCERFKCPCPTCGTENIYDNVFDGSGTDMEPSLYRCSNIDCKASPLTFTVQLSNKLIMDIRRFIKKYYDGWLICEEPTCRNRTRHLPLQFSRTGPLCPACMKATLQPEYSDKSLYTQLCFYRYIFDAECALEKLTTDHEKDKLKKQFFTPKVLQDYRKLKNTAEQFLSRSGYSEVNLSKLFAGCAVKS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
75
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P09884 As Substrate

Site PTM Type Enzyme
S9 Phosphorylation
S11 Phosphorylation
Y49 Phosphorylation
T55 Phosphorylation
Y58 Phosphorylation
Y66 Phosphorylation
K109 Ubiquitination
T129 Phosphorylation
K130 Acetylation
K130 Ubiquitination
K144 Acetylation
T174 Phosphorylation
S186 Phosphorylation
S190 Phosphorylation
S195 Phosphorylation
T198 Phosphorylation
T200 Phosphorylation
S209 Phosphorylation
S212 Phosphorylation
T219 Phosphorylation
K271 Acetylation
S277 Phosphorylation
K284 Sumoylation
S289 Phosphorylation
K402 Ubiquitination
T406 Phosphorylation
K411 Ubiquitination
K420 Ubiquitination
K449 Ubiquitination
Y452 Phosphorylation
K494 Ubiquitination
S503 Phosphorylation
K515 Ubiquitination
K522 Ubiquitination
K590 Ubiquitination
K599 Acetylation
K599 Ubiquitination
K604 Ubiquitination
K661 Ubiquitination
K829 Ubiquitination
K848 Ubiquitination
K930 Ubiquitination
Y942 Phosphorylation
K970 Ubiquitination
Y1000 Phosphorylation
K1031 Ubiquitination
K1054 Ubiquitination
Y1055 Phosphorylation
K1071 Ubiquitination
K1075 Ubiquitination
K1089 Ubiquitination
K1114 Ubiquitination
K1141 Ubiquitination
K1200 Ubiquitination
T1268 Phosphorylation
K1272 Ubiquitination
S1327 Phosphorylation
K1376 Ubiquitination
K1419 Ubiquitination
K1434 Methylation
K1434 Ubiquitination
S1462 Phosphorylation

Research Backgrounds

Function:

Catalytic subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, a regulatory subunit POLA2 and two primase subunits PRIM1 and PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. In the cytosol, responsible for a substantial proportion of the physiological concentration of cytosolic RNA:DNA hybrids, which are necessary to prevent spontaneous activation of type I interferon responses.

PTMs:

A 165 kDa form is probably produced by proteolytic cleavage at Lys-124.

Subcellular Location:

Nucleus. Cytoplasm>Cytosol.
Note: In the cytosol, colocalizes with RNA:DNA hybrids with a speckled pattern.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the alpha DNA polymerase complex (also known as the alpha DNA polymerase-primase complex) consisting of four subunits: the catalytic subunit POLA1, the regulatory subunit POLA2, and the primase complex subunits PRIM1 and PRIM2 respectively. Interacts with PARP1; this interaction functions as part of the control of replication fork progression. Interacts with MCM10 and WDHD1; these interactions recruit the polymerase alpha complex to the pre-replicative complex bound to DNA. Interacts with RPA1; this interaction stabilizes the replicative complex and reduces the misincorporation rate of DNA polymerase alpha by acting as a fidelity clamp.

(Microbial infection) Interacts with SV40 Large T antigen; this interaction allows viral DNA replication.

(Microbial infection) Interacts with herpes simplex virus 1/HHV-1 replication origin-binding protein UL9.

Family&Domains:

The CysA-type zinc finger is required for PCNA-binding.

The CysB motif binds 1 4Fe-4S cluster and is required for the formation of polymerase complexes.

Belongs to the DNA polymerase type-B family.

Research Fields

· Genetic Information Processing > Replication and repair > DNA replication.

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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