HOXC6 Antibody | Affinity Biosciences

WB
IHC
Cites

1/3

Western blot analysis of extracts from C6 cells, using HOXC6 Antibody.

DF3078 at 1/100 staining Human colorectal cancer by IHC-P.

Figure 5 Tumor cell biology experiments of HOXC6.

Product:	HOXC6 Antibody
Catalog:	DF3078
Description:	Rabbit polyclonal antibody to HOXC6
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	26 KD; 27kD(Calculated).
Uniprot:	P09630
RRID:	AB_2835461

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

HOXC6 Antibody detects endogenous levels of total HOXC6.

RRID:

AB_2835461
Cite Format: Affinity Biosciences Cat# DF3078, RRID:AB_2835461.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CP25; HHO.C8; Homeo box 3C; Homeo box C6; Homeo box C8 protein; Homeobox C6; Homeobox protein CP25; Homeobox protein HHO.C8; Homeobox protein Hox-3C; Homeobox protein Hox-C6; HOX3; HOX3C; Hoxc6; HXC6_HUMAN;

Immunogens

Immunogen:

Uniprot:

P09630(HXC6_HUMAN) >>Visit HPA database.

Gene(ID):

HOXC6(3223)

Sequence:

P09630 HXC6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNSYFTNPSLSCHLAGGQDVLPNVALNSTAYDPVRHFSTYGAAVAQNRIYSTPFYSPQENVVFSSSRGPYDYGSNSFYQEKDMLSNCRQNTLGHNTQTSIAQDFSSEQGRTAPQDQKASIQIYPWMQRMNSHSGVGYGADRRRGRQIYSRYQTLELEKEFHFNRYLTRRRRIEIANALCLTERQIKIWFQNRRMKWKKESNLTSTLSGGGGGATADSLGGKEEKREETEEEKQKE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Sheep

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P09630 As Substrate

P09630

Site	PTM Type	Source
S131	Phosphorylation	Uniprot
Y137	Phosphorylation	Uniprot
K158	Ubiquitination	Uniprot
R164	Methylation	Uniprot
K198	Acetylation	Uniprot
K198	Ubiquitination	Uniprot
K221	Acetylation	Uniprot

Research Backgrounds

P09630_HXC6_HUMAN

Function:

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.

Subcellular Location:

Nucleus.

Family&Domains:

Belongs to the Antp homeobox family.

References

1). Expression Profiles of HOXC6 Predict the Survival of Glioblastoma Patients and Correlate with Cell Cycle. Journal of Oncology, 2022 (PubMed: 35432534)

Application: WB Species: Human Sample: LN229 cells

Figure 5 Tumor cell biology experiments of HOXC6. (a) Expression of HOXC6 after transfection of siRNA or overexpression plasmid measured by PCR. (b) CCK-8 assay to evaluate the effect of HOXC6 knockdown or overexpression on cell proliferation in LN229 cells. ∗p < 0.05, ∗∗p < 0.01. (c) CCK-8 assay to evaluate the effect of HOXC6 knockdown or overexpression on cell proliferation in T98G cells. From left to right: NC, OE, and SI. (d) Flow cytometry analysis to evaluate the effects of HOXC6 on cell cycle progression in LN229 cells. (e) Flow cytometry analysis to evaluate the effects of HOXC6 on cell cycle progression in T98G cells. From left to right: NC, OE, and SI. (f) Western blot analysis to evaluate the effects of HOXC6 on expression level of cell cycle related proteins.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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HOXC6 Antibody - #DF3078