HEXB Antibody | Affinity Biosciences

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Western blot analysis of extracts from Rat muscle, using HEXB Antibody.

Product:	HEXB Antibody
Catalog:	DF3074
Description:	Rabbit polyclonal antibody to HEXB
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	63 KD; 63kD(Calculated).
Uniprot:	P07686
RRID:	AB_2835457

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

HEXB Antibody detects endogenous levels of total HEXB.

RRID:

AB_2835457
Cite Format: Affinity Biosciences Cat# DF3074, RRID:AB_2835457.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Beta hexosaminidase beta chain; Beta hexosaminidase subunit beta; Beta N acetylhexosaminidase; Beta-hexosaminidase subunit beta chain A; Beta-N-acetylhexosaminidase subunit beta; Cervical cancer proto oncogene 7 protein; Cervical cancer proto-oncogene 7 protein; ENC 1AS; Epididymis luminal protein 248; HCC 7; HCC-7; HCC7; HEL 248; HEX B; Hexb; HEXB_HUMAN; Hexosaminidase B (beta polypeptide); Hexosaminidase B; Hexosaminidase subunit B; HexosaminidaseB; N acetyl beta glucosaminidase; N-acetyl-beta-glucosaminidase subunit beta;

Immunogens

Immunogen:

Uniprot:

P07686(HEXB_HUMAN) >>Visit HPA database.

Gene(ID):

HEXB(3074)

Sequence:

P07686 HEXB_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MELCGLGLPRPPMLLALLLATLLAAMLALLTQVALVVQVAEAARAPSVSAKPGPALWPLPLSVKMTPNLLHLAPENFYISHSPNSTAGPSCTLLEEAFRRYHGYIFGFYKWHHEPAEFQAKTQVQQLLVSITLQSECDAFPNISSDESYTLLVKEPVAVLKANRVWGALRGLETFSQLVYQDSYGTFTINESTIIDSPRFSHRGILIDTSRHYLPVKIILKTLDAMAFNKFNVLHWHIVDDQSFPYQSITFPELSNKGSYSLSHVYTPNDVRMVIEYARLRGIRVLPEFDTPGHTLSWGKGQKDLLTPCYSRQNKLDSFGPINPTLNTTYSFLTTFFKEISEVFPDQFIHLGGDEVEFKCWESNPKIQDFMRQKGFGTDFKKLESFYIQKVLDIIATINKGSIVWQEVFDDKAKLAPGTIVEVWKDSAYPEELSRVTASGFPVILSAPWYLDLISYGQDWRKYYKVEPLDFGGTQKQKQLFIGGEACLWGEYVDATNLTPRLWPRASAVGERLWSSKDVRDMDDAYDRLTRHRCRMVERGIAAQPLYAGYCNHENM

PTMs - P07686 As Substrate

P07686

Site	PTM Type	Source
K51	Ubiquitination	Uniprot
N84	N-Glycosylation	Uniprot
N142	N-Glycosylation	Uniprot
K161	Ubiquitination	Uniprot
N190	N-Glycosylation	Uniprot
S201	Phosphorylation	Uniprot
T209	Phosphorylation	Uniprot
S210	Phosphorylation	Uniprot
T267	Phosphorylation	Uniprot
K303	Ubiquitination	Uniprot
N327	N-Glycosylation	Uniprot
K374	Ubiquitination	Uniprot
K412	Ubiquitination	Uniprot
S427	Phosphorylation	Uniprot
Y429	Phosphorylation	Uniprot
K465	Ubiquitination	Uniprot
T474	Phosphorylation	Uniprot
K476	Ubiquitination	Uniprot
R512	Methylation	Uniprot

Research Backgrounds

P07686_HEXB_HUMAN

Function:

Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues.

PTMs:

N-linked glycans at Asn-142 and Asn-190 consist of Man(3)-GlcNAc(2) and Man(5 to 7)-GlcNAc(2), respectively.

The beta-A and beta-B chains are produced by proteolytic processing of the precursor beta chain.

Subcellular Location:

Lysosome.

Subunit Structure:

There are 3 forms of beta-hexosaminidase: hexosaminidase A is a trimer composed of one subunit alpha, one subunit beta chain A and one subunit beta chain B; hexosaminidase B is a tetramer of two subunit beta chains A and two subunit beta chains B; hexosaminidase S is a homodimer of two alpha subunits. The two beta chains are derived from the cleavage of the beta subunit.

Family&Domains:

Belongs to the glycosyl hydrolase 20 family.

Research Fields

· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)

· Metabolism > Glycan biosynthesis and metabolism > Other glycan degradation.

· Metabolism > Carbohydrate metabolism > Amino sugar and nucleotide sugar metabolism.

· Metabolism > Glycan biosynthesis and metabolism > Glycosaminoglycan degradation.

· Metabolism > Glycan biosynthesis and metabolism > Glycosphingolipid biosynthesis - globo and isoglobo series.

· Metabolism > Glycan biosynthesis and metabolism > Glycosphingolipid biosynthesis - ganglio series.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

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HEXB Antibody - #DF3074