M-CK Antibody - #DF3072

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Product: M-CK Antibody
Catalog: DF3072
Description: Rabbit polyclonal antibody to M-CK
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 43 KD; 43kD(Calculated).
Uniprot: P06732
RRID: AB_2835455

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Bovine(91%), Sheep(91%), Rabbit(91%), Dog(91%), Chicken(89%)
Clonality:
Polyclonal
Specificity:
M-CK Antibody detects endogenous levels of total M-CK.
RRID:
AB_2835455
Cite Format: Affinity Biosciences Cat# DF3072, RRID:AB_2835455.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CKM; CKMM; Creatine kinase M; Creatine kinase M chain; Creatine kinase M type; Creatine kinase M-type; Creatine kinase muscle; Creatine kinase, muscle type; KCRM_HUMAN; M-CK; MCK; Muscle creatine kinase;

Immunogens

Immunogen:
Uniprot:

P06732(KCRM_HUMAN) >>Visit HPA database.

Gene(ID):
CKM(1158)
Sequence:
MPFGNTHNKFKLNYKPEEEYPDLSKHNNHMAKVLTLELYKKLRDKETPSGFTVDDVIQTGVDNPGHPFIMTVGCVAGDEESYEVFKELFDPIISDRHGGYKPTDKHKTDLNHENLKGGDDLDPNYVLSSRVRTGRSIKGYTLPPHCSRGERRAVEKLSVEALNSLTGEFKGKYYPLKSMTEKEQQQLIDDHFLFDKPVSPLLLASGMARDWPDARGIWHNDNKSFLVWVNEEDHLRVISMEKGGNMKEVFRRFCVGLQKIEEIFKKAGHPFMWNQHLGYVLTCPSNLGTGLRGGVHVKLAHLSKHPKFEEILTRLRLQKRGTGGVDTAAVGSVFDVSNADRLGSSEVEQVQLVVDGVKLMVEMEKKLEKGQSIDDMIPAQK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Bovine
91
Sheep
91
Dog
91
Rabbit
91
Chicken
89
Xenopus
78
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P06732 As Substrate

Site PTM Type Enzyme
Y14 Phosphorylation
Y20 Phosphorylation
S24 Phosphorylation
T35 Phosphorylation
Y39 Phosphorylation
K40 Acetylation
K41 Acetylation
Y125 Phosphorylation
S128 Phosphorylation
S129 Phosphorylation
S136 Phosphorylation
Y140 Phosphorylation
T141 Phosphorylation
S164 Phosphorylation
T166 Phosphorylation
K170 Acetylation
Y173 Phosphorylation
Y174 Phosphorylation
S178 Phosphorylation
T180 Phosphorylation
S199 Phosphorylation
R215 Methylation
S224 Phosphorylation
K247 Acetylation
K265 Acetylation
Y279 Phosphorylation
T313 Phosphorylation
T322 Phosphorylation
T327 Phosphorylation
S332 Phosphorylation
K365 Acetylation
S372 Phosphorylation

Research Backgrounds

Function:

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Dimer of identical or non-identical chains, which can be either B (brain type) or M (muscle type). With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.

Family&Domains:

Belongs to the ATP:guanido phosphotransferase family.

Research Fields

· Metabolism > Amino acid metabolism > Arginine and proline metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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For Research Use Only.
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