GAK Antibody - #AF0317

(3)  
Product: GAK Antibody
Catalog: AF0317
Description: Rabbit polyclonal antibody to GAK
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 144kDa; 143kD(Calculated).
Uniprot: O14976
RRID: AB_2833481

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Bovine(82%), Horse(82%), Sheep(82%), Rabbit(82%), Dog(82%)
Clonality:
Polyclonal
Specificity:
GAK Antibody detects endogenous levels of total GAK.
RRID:
AB_2833481
Cite Format: Affinity Biosciences Cat# AF0317, RRID:AB_2833481.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Auxilin 2; Cyclin G associated kinase; Cyclin-G-associated kinase; DNAJ26; DNAJC26; EC 2.7.11.1; FLJ16629; FLJ40395; GAK; GAK protein; GAK_HUMAN; MGC99654;

Immunogens

Immunogen:
Uniprot:

O14976(GAK_HUMAN) >>Visit HPA database.

Gene(ID):
GAK(2580)
Expression:
O14976 GAK_HUMAN:

Ubiquitous. Highest in testis.

Description:
GAK Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin- coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. Belongs to the protein kinase superfamily.
Sequence:
MSLLQSALDFLAGPGSLGGASGRDQSDFVGQTVELGELRLRVRRVLAEGGFAFVYEAQDVGSGREYALKRLLSNEEEKNRAIIQEVCFMKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVEFLKKMESRGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIIDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPPHDTQYTVFHSLIRAMLQVNPEERLSIAEVVHQLQEIAAARNVNPKSPITELLEQNGGYGSATLSRGPPPPVGPAGSGYSGGLALAEYDQPYGGFLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESALKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSRFHNRVSECGWAARRAPHLHTLYNICRNMHAWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVRAVVMTPVPLFSKQRSGCRPFCEVYVGDERVASTSQEYDKMRDFKIEDGKAVIPLGVTVQGDVLIVIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNLEVEVEPRDRPSREAPPWENSSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAGAGSPEAEPTDSDSPPSSSADASRFLHTLDWQEEKEAETGAENASSKESESALMEDRDESEVSDEGGSPISSEGQEPRADPEPPGLAAGLVQQDLVFEVETPAVLPEPVPQEDGVDLLGLHSEVGAGPAVPPQACKAPSSNTDLLSCLLGPPEAASQGPPEDLLSEDPLLLASPAPPLSVQSTPRGGPPAAADPFGPLLPSSGNNSQPCSNPDLFGEFLNSDSVTVPPSFPSAHSAPPPSCSADFLHLGDLPGEPSKMTASSSNPDLLGGWAAWTETAASAVAPTPATEGPLFSPGGQPAPCGSQASWTKSQNPDPFADLGDLSSGLQGSPAGFPPGGFIPKTATTPKGSSSWQTSRPPAQGASWPPQAKPPPKACTQPRPNYASNFSVIGAREERGVRAPSFAQKPKVSENDFEDLLSNQGFSSRSDKKGPKTIAEMRKQDLAKDTDPLKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVGMADLVAPEQVKKHYRRAVLAVHPDKAAGQPYEQHAKMIFMELNDAWSEFENQGSRPLF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
82
Bovine
82
Sheep
82
Dog
82
Rabbit
82
Chicken
73
Zebrafish
55
Pig
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14976 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S16 Phosphorylation
S26 Phosphorylation
S73 Phosphorylation
K78 Ubiquitination
K90 Ubiquitination
K91 Ubiquitination
K127 Ubiquitination
K135 Ubiquitination
Y153 Phosphorylation
K166 Ubiquitination
K175 Ubiquitination
T186 Phosphorylation
Y201 Phosphorylation
Y204 Phosphorylation
Y235 Phosphorylation
Y276 Phosphorylation
S277 Phosphorylation
Y285 Phosphorylation
S290 Phosphorylation
K325 Ubiquitination
Y338 Phosphorylation
S340 Phosphorylation
Y367 Phosphorylation
Y371 Phosphorylation
K390 Ubiquitination
K395 Acetylation
K395 Ubiquitination
Y403 Phosphorylation
K405 Ubiquitination
Y412 Phosphorylation P12931 (SRC)
S421 Phosphorylation
K432 Ubiquitination
R439 Methylation
K445 Ubiquitination
S456 Phosphorylation
S540 Phosphorylation
T583 Phosphorylation
S589 Phosphorylation
K590 Ubiquitination
Y615 Phosphorylation
K617 Ubiquitination
K682 Ubiquitination
K685 Ubiquitination
K748 Ubiquitination
K751 Ubiquitination
Y764 Phosphorylation
S770 Phosphorylation
T776 Phosphorylation
S778 Phosphorylation
S780 Phosphorylation
S783 Phosphorylation
S784 Phosphorylation
S785 Phosphorylation
T794 Phosphorylation
K801 Ubiquitination
T805 Phosphorylation
S811 Phosphorylation
S812 Phosphorylation
S815 Phosphorylation
S817 Phosphorylation
S826 Phosphorylation
S829 Phosphorylation
S834 Phosphorylation
S837 Phosphorylation
S838 Phosphorylation
T908 Phosphorylation
S939 Phosphorylation
S1096 Phosphorylation
S1118 Phosphorylation
S1130 Phosphorylation
Y1149 Phosphorylation
S1151 Phosphorylation
S1154 Phosphorylation
S1168 Phosphorylation
K1172 Ubiquitination
S1176 Phosphorylation
S1185 Phosphorylation
R1192 Methylation
T1213 Phosphorylation
K1217 Ubiquitination
K1219 Ubiquitination
K1278 Ubiquitination

Research Backgrounds

Function:

Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.

Subcellular Location:

Cytoplasm>Perinuclear region. Golgi apparatus>trans-Golgi network. Cell junction>Focal adhesion.
Note: Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Highest in testis.

Family&Domains:

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

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