MOS Antibody | Affinity Biosciences

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Western blot analysis of extracts from 293 cells, using MOS antibody.

Product:	MOS Antibody
Catalog:	DF3067
Description:	Rabbit polyclonal antibody to MOS
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Zebrafish, Bovine, Chicken, Xenopus
Mol.Wt.:	40 KD; 38kD(Calculated).
Uniprot:	P00540
RRID:	AB_2835450

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Zebrafish(88%), Bovine(100%), Chicken(88%), Xenopus(88%)

Clonality:

Polyclonal

Specificity:

MOS Antibody detects endogenous levels of total MOS.

RRID:

AB_2835450
Cite Format: Affinity Biosciences Cat# DF3067, RRID:AB_2835450.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

c mos; MGC119962; MGC119963; MOS; MOS_HUMAN; MSV; Oocyte maturation factor mos; Proto oncogene serine / threonine protein kinase mos; Proto-oncogene c-Mos; Proto-oncogene serine/threonine-protein kinase mos; v mos Moloney murine sarcoma viral oncogene homolog;

Immunogens

Immunogen:

Uniprot:

P00540(MOS_HUMAN) >>Visit HPA database.

Gene(ID):

MOS(4342)

Expression:

P00540 MOS_HUMAN:

Expressed specifically in testis during spermatogenesis.

Sequence:

P00540 MOS_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPSPLALRPYLRSEFSPSVDARPCSSPSELPAKLLLGATLPRAPRLPRRLAWCSIDWEQVCLLQRLGAGGFGSVYKATYRGVPVAIKQVNKCTKNRLASRRSFWAELNVARLRHDNIVRVVAASTRTPAGSNSLGTIIMEFGGNVTLHQVIYGAAGHPEGDAGEPHCRTGGQLSLGKCLKYSLDVVNGLLFLHSQSIVHLDLKPANILISEQDVCKISDFGCSEKLEDLLCFQTPSYPLGGTYTHRAPELLKGEGVTPKADIYSFAITLWQMTTKQAPYSGERQHILYAVVAYDLRPSLSAAVFEDSLPGQRLGDVIQRCWRPSAAQRPSARLLLVDLTSLKAELG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Xenopus

Zebrafish

Chicken

Pig

Horse

Sheep

Dog

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P00540 As Substrate

P00540

Site	PTM Type	Source
S3	Phosphorylation	Uniprot
S16	Phosphorylation	Uniprot
Y263	Phosphorylation	Uniprot
T339	Phosphorylation	Uniprot
S340	Phosphorylation	Uniprot

PTMs - P00540 As Enzyme

P00540

Substrate	Site	Source
P08670 (VIM)	T458	Uniprot
P08670 (VIM)	S459	Uniprot
Q02750 (MAP2K1)	S218	Uniprot
Q02750 (MAP2K1)	S222	Uniprot

Research Backgrounds

P00540_MOS_HUMAN

Tissue Specificity:

Expressed specifically in testis during spermatogenesis.

Family&Domains:

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Research Fields

· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

MOS Antibody - #DF3067