CBX6 Antibody | Affinity Biosciences

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Western blot analysis of extracts from 293 cells using CBX6 antibody.

Product:	CBX6 Antibody
Catalog:	DF3060
Description:	Rabbit polyclonal antibody to CBX6
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Chicken, Xenopus
Mol.Wt.:	44 KD; 44kD(Calculated).
Uniprot:	O95503
RRID:	AB_2835443

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Chicken(88%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

CBX6 Antibody detects endogenous levels of total CBX6.

RRID:

AB_2835443
Cite Format: Affinity Biosciences Cat# DF3060, RRID:AB_2835443.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Cbx 6; Cbx6; CBX6_HUMAN; Chromobox homolog 6; Chromobox protein homolog 6;

Immunogens

Immunogen:

Uniprot:

O95503(CBX6_HUMAN) >>Visit HPA database.

Gene(ID):

CBX6(23466)

Sequence:

O95503 CBX6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MELSAVGERVFAAESIIKRRIRKGRIEYLVKWKGWAIKYSTWEPEENILDSRLIAAFEQKERERELYGPKKRGPKPKTFLLKARAQAEALRISDVHFSVKPSASASSPKLHSSAAVHRLKKDIRRCHRMSRRPLPRPDPQGGSPGLRPPISPFSETVRIINRKVKPREPKRNRIILNLKVIDKGAGGGGAGQGAGALARPKVPSRNRVIGKSKKFSESVLRTQIRHMKFGAFALYKPPPAPLVAPSPGKAEASAPGPGLLLAAPAAPYDARSSGSSGCPSPTPQSSDPDDTPPKLLPETVSPSAPSWREPEVLDLSLPPESAATSKRAPPEVTAAAGPAPPTAPEPAGASSEPEAGDWRPEMSPCSNVVVTDVTSNLLTVTIKEFCNPEDFEKVAAGVAGAAGGGGSIGASK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Xenopus

100

Chicken

Horse

Sheep

Dog

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95503 As Substrate

O95503

Site	PTM Type	Source
K18	Ubiquitination	Uniprot
K33	Ubiquitination	Uniprot
K38	Ubiquitination	Uniprot
Y39	Phosphorylation	Uniprot
K60	Ubiquitination	Uniprot
K82	Ubiquitination	Uniprot
K100	Acetylation	Uniprot
K100	Ubiquitination	Uniprot
S106	Phosphorylation	Uniprot
S107	Phosphorylation	Uniprot
S130	Phosphorylation	Uniprot
S143	Phosphorylation	Uniprot
S151	Phosphorylation	Uniprot
T156	Phosphorylation	Uniprot
K179	Ubiquitination	Uniprot
K183	Ubiquitination	Uniprot
K214	Ubiquitination	Uniprot
K236	Ubiquitination	Uniprot
S246	Phosphorylation	Uniprot
S280	Phosphorylation	Uniprot
T282	Phosphorylation	Uniprot
S285	Phosphorylation	Uniprot
S301	Phosphorylation	Uniprot
S303	Phosphorylation	Uniprot

Research Backgrounds

O95503_CBX6_HUMAN

Function:

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Possibly contributes to the target selectivity of the PRC1 complex by binding specific regions of chromatin. Recruitment to chromatin might occur in an H3K27me3-independent fashion (By similarity). May have a PRC1-independent function in embryonic stem cells (By similarity).

Subcellular Location:

Nucleus. Chromosome.
Note: Uniformely distributed in the nucleoplasm (PubMed:18927235). Localizes to the inactivated X chromosome in females (By similarity).

Subunit Structure:

Component of a PRC1-like complex. Distinct PRC1-like core complexes are composed of a RING1 subunit (RING1B or RING1A), one of the six PCGF proteins (PCGF1-6), one PHC protein (PHC1-3) and one of the CBX proteins (CBX2, CBX4, CBX6, CBX7 or CBX8). Interacts with PCGF1, PCGF2, PCGF3, BMI1, PCGF5, PCGF6, RING1 and RNF2. May interact with HIST2H3A and HIST1H3A. Interacts (via chromodomain) with single-stranded RNA (ssRNA) (By similarity).

Restrictive clause

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For Research Use Only.
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CBX6 Antibody - #DF3060