Product: SIX6 Antibody
Catalog: DF3059
Description: Rabbit polyclonal antibody to SIX6
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 28 KD; 28kD(Calculated).
Uniprot: O95475
RRID: AB_2835442

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
SIX6 Antibody detects endogenous levels of total SIX6.
RRID:
AB_2835442
Cite Format: Affinity Biosciences Cat# DF3059, RRID:AB_2835442.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Homeobox protein SIX6; Homeodomain protein OPTX2; MCOPCT 2; MCOPCT2; Optic homeobox 2; OPTX 2; OPTX2; Sine oculis homeobox homolog 6; SIX 6; Six 9; SIX homeobox 6; Six6; SIX6_HUMAN; Six9;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O95475 SIX6_HUMAN:

Expressed in the developing and adult retina. Also expressed in the hypothalamic and the pituitary regions.

Sequence:
MFQLPILNFSPQQVAGVCETLEESGDVERLGRFLWSLPVAPAACEALNKNESVLRARAIVAFHGGNYRELYHILENHKFTKESHAKLQALWLEAHYQEAEKLRGRPLGPVDKYRVRKKFPLPRTIWDGEQKTHCFKERTRHLLREWYLQDPYPNPSKKRELAQATGLTPTQVGNWFKNRRQRDRAAAAKNRLQQQVLSQGSGRALRAEGDGTPEVLGVATSPAASLSSKAATSAISITSSDSECDI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Bovine
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95475 As Substrate

Site PTM Type Enzyme
S10 Phosphorylation
K49 Ubiquitination
Y96 Phosphorylation
K112 Ubiquitination
K131 Ubiquitination
K136 Ubiquitination
Y147 Phosphorylation
Y152 Phosphorylation
S156 Phosphorylation
S198 Phosphorylation
S201 Phosphorylation
T212 Phosphorylation
T220 Phosphorylation
S221 Phosphorylation
S225 Phosphorylation
S227 Phosphorylation
S228 Phosphorylation

Research Backgrounds

Function:

May be involved in eye development.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the developing and adult retina. Also expressed in the hypothalamic and the pituitary regions.

Subunit Structure:

Interacts with TLE4 and TLE5.

Family&Domains:

Belongs to the SIX/Sine oculis homeobox family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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