MYST2 Antibody - #DF3056

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Product: MYST2 Antibody
Catalog: DF3056
Description: Rabbit polyclonal antibody to MYST2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 75 KD; 71kD(Calculated).
Uniprot: O95251
RRID: AB_2835439

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
MYST2 Antibody detects endogenous levels of total MYST2.
RRID:
AB_2835439
Cite Format: Affinity Biosciences Cat# DF3056, RRID:AB_2835439.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Hbo 1; HBO1; HBOa; Histone acetyltransferase binding to hORC1; Histone acetyltransferase binding to ORC1; Histone acetyltransferase KAT7; Histone acetyltransferase MYST2; K(lysine) acetyltransferase 7; KAT 7; KAT7; Lysine acetyltransferase 7; MOZ; MOZ YBF2/SAS3 SAS2 and TIP60 protein; MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2; MYST 2; MYST histone acetyltransferase 2; MYST protein 2; MYST-2; MYST2; MYST2_HUMAN; SAS 2; SAS2 and TIP60 protein 2; TIP60 protein 2; YBF2/SAS3; ZC2HC7;

Immunogens

Immunogen:
Uniprot:

O95251(KAT7_HUMAN) >>Visit HPA database.

Gene(ID):
KAT7(11143)
Expression:
O95251 KAT7_HUMAN:

Ubiquitously expressed, with highest levels in testis.

Sequence:
MPRRKRNAGSSSDGTEDSDFSTDLEHTDSSESDGTSRRSARVTRSSARLSQSSQDSSPVRNLQSFGTEEPAYSTRRVTRSQQQPTPVTPKKYPLRQTRSSGSETEQVVDFSDRETKNTADHDESPPRTPTGNAPSSESDIDISSPNVSHDESIAKDMSLKDSGSDLSHRPKRRRFHESYNFNMKCPTPGCNSLGHLTGKHERHFSISGCPLYHNLSADECKVRAQSRDKQIEERMLSHRQDDNNRHATRHQAPTERQLRYKEKVAELRKKRNSGLSKEQKEKYMEHRQTYGNTREPLLENLTSEYDLDLFRRAQARASEDLEKLRLQGQITEGSNMIKTIAFGRYELDTWYHSPYPEEYARLGRLYMCEFCLKYMKSQTILRRHMAKCVWKHPPGDEIYRKGSISVFEVDGKKNKIYCQNLCLLAKLFLDHKTLYYDVEPFLFYVMTEADNTGCHLIGYFSKEKNSFLNYNVSCILTMPQYMRQGYGKMLIDFSYLLSKVEEKVGSPERPLSDLGLISYRSYWKEVLLRYLHNFQGKEISIKEISQETAVNPVDIVSTLQALQMLKYWKGKHLVLKRQDLIDEWIAKEAKRSNSNKTMDPSCLKWTPPKGT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95251 As Substrate

Site PTM Type Enzyme
S10 Phosphorylation
T15 Phosphorylation
S18 Phosphorylation
T22 Phosphorylation
S39 Phosphorylation
S45 Phosphorylation
S46 Phosphorylation
S50 Phosphorylation
S52 Phosphorylation
S53 Phosphorylation
S56 Phosphorylation
S57 Phosphorylation P53350 (PLK1)
S64 Phosphorylation
T67 Phosphorylation
Y72 Phosphorylation
T74 Phosphorylation
T78 Phosphorylation
S80 Phosphorylation
T85 Phosphorylation P06493 (CDK1)
T88 Phosphorylation P06493 (CDK1) , P24941 (CDK2)
K90 Ubiquitination
T97 Phosphorylation
S99 Phosphorylation
S100 Phosphorylation
S102 Phosphorylation
T104 Phosphorylation
S111 Phosphorylation
T115 Phosphorylation
T118 Phosphorylation
S124 Phosphorylation
T128 Phosphorylation
T130 Phosphorylation
S135 Phosphorylation
S136 Phosphorylation
S138 Phosphorylation
S158 Phosphorylation
K160 Acetylation
S162 Phosphorylation
S164 Phosphorylation
S167 Phosphorylation
K171 Acetylation
S178 Phosphorylation
K184 Sumoylation
T187 Phosphorylation
S192 Phosphorylation
K199 Acetylation
S205 Phosphorylation
Y212 Phosphorylation
S216 Phosphorylation
K221 Acetylation
K221 Ubiquitination
K229 Acetylation
K261 Acetylation
K269 Acetylation
K270 Acetylation
K277 Acetylation
K323 Acetylation
K323 Ubiquitination
T331 Phosphorylation
S334 Phosphorylation
K338 Ubiquitination
T339 Phosphorylation
K376 Methylation
K376 Ubiquitination
Y399 Phosphorylation
S403 Phosphorylation
K432 Acetylation
S466 Phosphorylation
S506 Phosphorylation
S512 Phosphorylation
K524 Ubiquitination
K537 Ubiquitination
S540 Phosphorylation
S545 Phosphorylation
K587 Ubiquitination
K596 Acetylation
T606 Phosphorylation
K609 Ubiquitination
T611 Phosphorylation

Research Backgrounds

Function:

Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Involved in H3K14 (histone H3 lysine 14) acetylation and cell proliferation (By similarity). Through chromatin acetylation it may regulate DNA replication and act as a coactivator of TP53-dependent transcription. Acts as a coactivator of the licensing factor CDT1. Specifically represses AR-mediated transcription.

PTMs:

Phosphorylation at Ser-57 by PLK1 during mitosis seems important for prereplicative complex formation and DNA replication licensing, and requires prior phosphorylation at Thr-85 and Thr-88 by CDK1. Phosphorylated by MAP2K1, which accelerates its degradation (By similarity).

Autoacetylation at Lys-432 is required for proper function.

Ubiquitinated at Lys-338, leading to proteasomal degradation.

Subcellular Location:

Nucleus>Nucleoplasm. Cytoplasm>Cytosol. Nucleus.
Note: Associates with replication origins specifically during the G1 phase of the cell cycle (PubMed:18832067).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed, with highest levels in testis.

Subunit Structure:

Component of the HBO1 complex composed at least of ING4 or ING5, KAT7/HBO1, MEAF6, and one of JADE1, JADE2 and JADE3. Interacts with MCM2 and ORC1. Interacts with the androgen receptor (AR) in the presence of dihydrotestosterone. Interacts with CDT1. Interacts with MAP2K1 and CUL1 (By similarity).

Family&Domains:

The C2HC MYST-type zinc finger is required for interaction with MCM2 and ORC1.

The N-terminus is involved in transcriptional repression, while the C-terminus mediates AR-interaction.

Belongs to the MYST (SAS/MOZ) family.

References

1). Histone Acetyltransferase Mof Affects the Progression of DSS-Induced Colitis. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2018 (PubMed: 29975939)

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