TBX10 Antibody | Affinity Biosciences

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Western blot analysis of extracts from HT-29 cells, using TBX10 antibody.

Product:	TBX10 Antibody
Catalog:	DF3039
Description:	Rabbit polyclonal antibody to TBX10
Application:	WB IF/ICC
Reactivity:	Human
Mol.Wt.:	43 KD; 42kD(Calculated).
Uniprot:	O75333
RRID:	AB_2835422

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

TBX10 Antibody detects endogenous levels of total TBX10.

RRID:

AB_2835422
Cite Format: Affinity Biosciences Cat# DF3039, RRID:AB_2835422.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Dc; MGC129316; MGC129317; MmTBX7; T BOX 10; T box protein 10; T box protein 13; T-box protein 10; T-box transcription factor TBX10; Tbx10; TBX10_HUMAN; Tbx13; Tbx7;

Immunogens

Immunogen:

Uniprot:

O75333(TBX10_HUMAN) >>Visit HPA database.

Gene(ID):

TBX10(347853)

Sequence:

O75333 TBX10_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAFLSAGLGILAPSETYPLPTTSSGWEPRLGSPFPSGPCTSSTGAQAVAEPTGQGPKNPRVSRVTVQLEMKPLWEEFNQLGTEMIVTKAGRRMFPPFQVKILGMDSLADYALLMDFIPLDDKRYRYAFHSSAWLVAGKADPATPGRVHFHPDSPAKGAQWMRQIVSFDKLKLTNNLLDDNGHIILNSMHRYQPRFHVVFVDPRKDSERYAQENFKSFIFTETQFTAVTAYQNHRITQLKIASNPFAKGFRESDLDSWPVAPRPLLSVPARSHSSLSPCVLKGATDREKDPNKASASTSKTPAWLHHQLLPPPEVLLAPATYRPVTYQSLYSGAPSHLGIPRTRPAPYPLPNIRADRDQGGLPLPAGLGLLSPTVVCLGPGQDSQ

PTMs - O75333 As Substrate

O75333

Site	PTM Type	Source
Y192	Phosphorylation	Uniprot
Y210	Phosphorylation	Uniprot
Y231	Phosphorylation	Uniprot
T237	Phosphorylation	Uniprot
S267	Phosphorylation	Uniprot
S275	Phosphorylation	Uniprot
S277	Phosphorylation	Uniprot

Research Backgrounds

O75333_TBX10_HUMAN

Function:

Probable transcriptional regulator involved in developmental processes.

Subcellular Location:

Nucleus.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

TBX10 Antibody - #DF3039