HOXA1 Antibody - #DF3187

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Product: HOXA1 Antibody
Catalog: DF3187
Description: Rabbit polyclonal antibody to HOXA1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit
Mol.Wt.: 37 KD; 37kD(Calculated).
Uniprot: P49639
RRID: AB_2835410

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(89%), Bovine(89%), Horse(89%), Sheep(89%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
HOXA1 Antibody detects endogenous levels of total HOXA1.
RRID:
AB_2835410
Cite Format: Affinity Biosciences Cat# DF3187, RRID:AB_2835410.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BSAS; Homeo box A1; Homeobox 1F; Homeobox A1; Homeobox protein Hox A1; Homeobox protein Hox-1F; Homeobox protein Hox-A1; Hox 1.6 like protein; Hox 1F; HOX A1; HOX A1 homeodomain protein; HOX1; HOX1F; hoxa1; hoxb1b; HXA1_HUMAN; Lab like protein; MGC45232;

Immunogens

Immunogen:
Uniprot:

P49639(HXA1_HUMAN) >>Visit HPA database.

Gene(ID):
HOXA1(3198)
Sequence:
MDNARMNSFLEYPILSSGDSGTCSARAYPSDHRITTFQSCAVSANSCGGDDRFLVGRGVQIGSPHHHHHHHHHHPQPATYQTSGNLGVSYSHSSCGPSYGSQNFSAPYSPYALNQEADVSGGYPQCAPAVYSGNLSSPMVQHHHHHQGYAGGAVGSPQYIHHSYGQEHQSLALATYNNSLSPLHASHQEACRSPASETSSPAQTFDWMKVKRNPPKTGKVGEYGYLGQPNAVRTNFTTKQLTELEKEFHFNKYLTRARRVEIAASLQLNETQVKIWFQNRRMKQKKREKEGLLPISPATPPGNDEKAEESSEKSSSSPCVPSPGSSTSDTLTTSH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
89
Horse
89
Bovine
89
Sheep
89
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49639 As Substrate

Site PTM Type Enzyme
Y28 Phosphorylation
S30 Phosphorylation
T255 Phosphorylation
S296 Phosphorylation
T299 Phosphorylation
S334 Phosphorylation

Research Backgrounds

Function:

Sequence-specific transcription factor (By similarity). Regulates multiple developmental processes including brainstem, inner and outer ear, abducens nerve and cardiovascular development and morphogenesis as well as cognition and behavior. Also part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Acts on the anterior body structures. Seems to act in the maintenance and/or generation of hindbrain segments (By similarity). Activates transcription in the presence of PBX1A and PKNOX1 (By similarity).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with OGT (via TPR repeats domain); the interaction takes place mainly in the nucleus.

Family&Domains:

Belongs to the Antp homeobox family. Labial subfamily.

References

1). Free fatty acid receptor 4 deletion attenuates colitis by modulating Treg Cells via ZBED6-IL33 pathway. eBioMedicine, 2022 (PubMed: 35525203) [IF=11.1]
2). HOXA1 participates in VSMC-to-macrophage-like cell transformation via regulation of NF-κB p65 and KLF4: a potential mechanism of atherosclerosis pathogenesis. Molecular Medicine, 2023 (PubMed: 37528397) [IF=5.7]

Application: IF/ICC    Species: Mouse    Sample:

Fig. 1 HOXA1 expression is increased in the aortic root of AS mice. A Oil red-O staining for the aortic root of wild-type mice with ND, wild-type mice with HFD, or ApoE−/− mice with HFD. Scale bar = 500 μm (40 ×). B Immunofluorescence double staining for HOXA1 (red) and α-SMA (green) in each group. DAPI (blue) was used for nucleus staining. Scale bar = 50 μm (400 ×). C Western blot for HOXA1 expression in aortic root of wild-type or HFD-fed ApoE−/− mice. Data are expressed as mean ± SD (6 animals/group). Ordinary one-way ANOVA followed by Tukey's multiple comparison test was used to calculate the P value in panel C ***P 

Application: WB    Species: Mouse    Sample:

Fig. 1 HOXA1 expression is increased in the aortic root of AS mice. A Oil red-O staining for the aortic root of wild-type mice with ND, wild-type mice with HFD, or ApoE−/− mice with HFD. Scale bar = 500 μm (40 ×). B Immunofluorescence double staining for HOXA1 (red) and α-SMA (green) in each group. DAPI (blue) was used for nucleus staining. Scale bar = 50 μm (400 ×). C Western blot for HOXA1 expression in aortic root of wild-type or HFD-fed ApoE−/− mice. Data are expressed as mean ± SD (6 animals/group). Ordinary one-way ANOVA followed by Tukey's multiple comparison test was used to calculate the P value in panel C

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