GRK6 Antibody - #DF3180
| Product: | GRK6 Antibody |
| Catalog: | DF3180 |
| Description: | Rabbit polyclonal antibody to GRK6 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus |
| Mol.Wt.: | 65 KD; 66kD(Calculated). |
| Uniprot: | P43250 |
| RRID: | AB_2835403 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF3180, RRID:AB_2835403.
Fold/Unfold
FLJ32135; G protein coupled receptor kinase 6; G protein coupled receptor kinase GRK6; G protein-coupled receptor kinase 6; G protein-coupled receptor kinase GRK6; Gprk6; Grk6; GRK6_HUMAN;
Immunogens
- P43250 GRK6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MELENIVANTVLLKAREGGGGNRKGKSKKWRQMLQFPHISQCEELRLSLERDYHSLCERQPIGRLLFREFCATRPELSRCVAFLDGVAEYEVTPDDKRKACGRQLTQNFLSHTGPDLIPEVPRQLVTNCTQRLEQGPCKDLFQELTRLTHEYLSVAPFADYLDSIYFNRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRIKKRKGEAMALNEKQILEKVNSRFVVSLAYAYETKDALCLVLTLMNGGDLKFHIYHMGQAGFPEARAVFYAAEICCGLEDLHRERIVYRDLKPENILLDDHGHIRISDLGLAVHVPEGQTIKGRVGTVGYMAPEVVKNERYTFSPDWWALGCLLYEMIAGQSPFQQRKKKIKREEVERLVKEVPEEYSERFSPQARSLCSQLLCKDPAERLGCRGGSAREVKEHPLFKKLNFKRLGAGMLEPPFKPDPQAIYCKDVLDIEQFSTVKGVELEPTDQDFYQKFATGSVPIPWQNEMVETECFQELNVFGLDGSVPPDLDWKGQPPAPPKKGLLQRLFSRQDCCGNCSDSEEELPTRL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P43250 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T10 | Phosphorylation | Uniprot | |
| K14 | Ubiquitination | Uniprot | |
| T106 | Phosphorylation | Uniprot | |
| S111 | Phosphorylation | Uniprot | |
| T113 | Phosphorylation | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| K183 | Ubiquitination | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| K210 | Ubiquitination | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| K235 | Acetylation | Uniprot | |
| K235 | Ubiquitination | Uniprot | |
| K240 | Acetylation | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| K313 | Ubiquitination | Uniprot | |
| K343 | Ubiquitination | Uniprot | |
| K358 | Ubiquitination | Uniprot | |
| K402 | Ubiquitination | Uniprot | |
| K426 | Ubiquitination | Uniprot | |
| K443 | Ubiquitination | Uniprot | |
| K466 | Ubiquitination | Uniprot | |
| S484 | Phosphorylation | P43250 (GRK6) | Uniprot |
| T485 | Phosphorylation | P43250 (GRK6) | Uniprot |
| K487 | Ubiquitination | Uniprot | |
| S557 | Phosphorylation | Uniprot | |
| S566 | Phosphorylation | Uniprot | |
| S568 | Phosphorylation | Uniprot |
PTMs - P43250 As Enzyme
| Substrate | Site | Source |
|---|---|---|
| O14745 (SLC9A3R1) | S290 | Uniprot |
| O75581 (LRP6) | S1448 | Uniprot |
| O75581 (LRP6) | S1490 | Uniprot |
| O75581 (LRP6) | S1508 | Uniprot |
| O75581 (LRP6) | T1572 | Uniprot |
| O75581 (LRP6) | S1590 | Uniprot |
| P07550 (ADRB2) | S355 | Uniprot |
| P07550 (ADRB2) | S356 | Uniprot |
| P08069 (IGF1R) | S1321 | Uniprot |
| P25963 (NFKBIA) | S32 | Uniprot |
| P25963 (NFKBIA) | S36 | Uniprot |
| P30411-2 (BDKRB2) | S346 | Uniprot |
| P30411-2 (BDKRB2) | S348 | Uniprot |
| P30411 (BDKRB2) | S366 | Uniprot |
| P30411 (BDKRB2) | S373 | Uniprot |
| P30411-1 (BDKRB2) | S375 | Uniprot |
| P37840-1 (SNCA) | S9 | Uniprot |
| P37840 (SNCA) | S129 | Uniprot |
| P43250 (GRK6) | S484 | Uniprot |
| P43250-3 (GRK6) | T485 | Uniprot |
| P61073 (CXCR4) | S324 | Uniprot |
| P61073 (CXCR4) | S325 | Uniprot |
| P61073 (CXCR4) | S330 | Uniprot |
| P61073 (CXCR4) | S339 | Uniprot |
| Q15722 (LTB4R) | T308 | Uniprot |
Research Backgrounds
Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (By similarity). Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro).
It is uncertain whether palmitoylation is on Cys-561 and/or Cys-562 and/or Cys-565.
Membrane>Lipid-anchor.
Widely expressed.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Research Fields
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Human Diseases > Substance dependence > Morphine addiction.
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
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