Product: FRK Antibody
Catalog: DF3179
Description: Rabbit polyclonal antibody to FRK
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Dog
Mol.Wt.: 55 KD; 58kD(Calculated).
Uniprot: P42685
RRID: AB_2835402

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Dog(91%)
Clonality:
Polyclonal
Specificity:
FRK Antibody detects endogenous levels of total FRK.
RRID:
AB_2835402
Cite Format: Affinity Biosciences Cat# DF3179, RRID:AB_2835402.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Beta cell Src homology tyrosine kinase; BSK; BSK/IYK; C85044; Frk; Frk fyn related kinase; FRK_HUMAN; FYN related kinase; FYN-related kinase; GASK; Gastrointestinal associated kinase; Gastrointestinal associated tyrosine kinase; GTK; GTK, mouse, homolog of; Intestine tyrosine kinase; Nuclear tyrosine protein kinase RAK; Protein-tyrosine kinase 5; PTK5; PTK5 protein tyrosine kinase 5; RAK; Tyrosine protein kinase FRK; Tyrosine-protein kinase FRK;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P42685 FRK_HUMAN:

Predominantly expressed in epithelial derived cell lines and tissues, especially normal liver, kidney, breast and colon.

Sequence:
MSNICQRLWEYLEPYLPCLSTEADKSTVIENPGALCSPQSQRHGHYFVALFDYQARTAEDLSFRAGDKLQVLDTLHEGWWFARHLEKRRDGSSQQLQGYIPSNYVAEDRSLQAEPWFFGAIGRSDAEKQLLYSENKTGSFLIRESESQKGEFSLSVLDGAVVKHYRIKRLDEGGFFLTRRRIFSTLNEFVSHYTKTSDGLCVKLGKPCLKIQVPAPFDLSYKTVDQWEIDRNSIQLLKRLGSGQFGEVWEGLWNNTTPVAVKTLKPGSMDPNDFLREAQIMKNLRHPKLIQLYAVCTLEDPIYIITELMRHGSLQEYLQNDTGSKIHLTQQVDMAAQVASGMAYLESRNYIHRDLAARNVLVGEHNIYKVADFGLARVFKVDNEDIYESRHEIKLPVKWTAPEAIRSNKFSIKSDVWSFGILLYEIITYGKMPYSGMTGAQVIQMLAQNYRLPQPSNCPQQFYNIMLECWNAEPKERPTFETLRWKLEDYFETDSSYSDANNFIR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
91
Chicken
45
Horse
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P42685 As Substrate

Site PTM Type Enzyme
S37 Phosphorylation
S40 Phosphorylation
Y46 Phosphorylation
Y53 Phosphorylation
T57 Phosphorylation
S62 Phosphorylation
S92 Phosphorylation
S93 Phosphorylation
Y99 Phosphorylation
S102 Phosphorylation
Y104 Phosphorylation
Y132 Phosphorylation
S133 Phosphorylation
S139 Phosphorylation
T178 Phosphorylation
S184 Phosphorylation
Y193 Phosphorylation
Y221 Phosphorylation
S233 Phosphorylation
Y303 Phosphorylation
Y317 Phosphorylation
Y368 Phosphorylation
Y387 Phosphorylation P42685 (FRK)
S389 Phosphorylation
S407 Phosphorylation
K409 Acetylation
S495 Phosphorylation
S496 Phosphorylation
Y497 Phosphorylation P41240 (CSK)
S498 Phosphorylation

PTMs - P42685 As Enzyme

Substrate Site Source
P38398 (BRCA1) Y1552 Uniprot
P42685 (FRK) Y387 Uniprot
P60484 (PTEN) Y336 Uniprot

Research Backgrounds

Function:

Non-receptor tyrosine-protein kinase that negatively regulates cell proliferation. Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation, possibly by reducing its binding to NEDD4. May function as a tumor suppressor.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Predominantly found in the nucleus, with a small fraction found in the cell periphery.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Predominantly expressed in epithelial derived cell lines and tissues, especially normal liver, kidney, breast and colon.

Subunit Structure:

Interacts (via the SH3-domain) with PTEN. Interacts with RB1.

Family&Domains:

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.

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