PFKFB1 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from HepG2, using PFKFB1 Antibody.

Product:	PFKFB1 Antibody
Catalog:	DF3098
Description:	Rabbit polyclonal antibody to PFKFB1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Zebrafish, Bovine, Horse, Sheep, Rabbit, Xenopus
Mol.Wt.:	58 KD; 55kD(Calculated).
Uniprot:	P16118
RRID:	AB_2835398

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Xenopus(82%)

Clonality:

Polyclonal

Specificity:

PFKFB1 Antibody detects endogenous levels of total PFKFB1.

RRID:

AB_2835398
Cite Format: Affinity Biosciences Cat# DF3098, RRID:AB_2835398.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

6 phosphofructo 2 kinase/ fructose 2,6 bisphosphatase; 6 phosphofructo 2 kinase/fructose 2,6 biphosphatase 3; 6-bisphosphatase; 6-P2ase 3; 6-P2ASE brain/placenta-type isozyme; 6PF 2 K/Fru 2,6 P2ASE brain/placenta type isozyme; 6PF 2-K/Fru 2,6 P2ase 3; 6PF-2-K/Fru-2; F263_HUMAN; fructose 6 phosphate,2 kinase/fructose 2, 6 bisphosphatase; Fructose-2; Inducible 6 phosphofructo 2 kinase/fructose 2,6 bisphosphatase; iPFK 2; iPFK-2; IPFK2; PFK/FBPase 3; PFK2; PFKFB3; Renal carcinoma antigen NY REN 56; Renal carcinoma antigen NY-REN-56; uPFK 2;

Immunogens

Immunogen:

Uniprot:

P16118(F261_HUMAN) >>Visit HPA database.

Gene(ID):

PFKFB1(5207)

Expression:

P16118 F261_HUMAN:

Liver.

Sequence:

P16118 F261_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSPEMGELTQTRLQKIWIPHSSGSSRLQRRRGSSIPQFTNSPTMVIMVGLPARGKTYISTKLTRYLNWIGTPTKVFNLGQYRREAVSYKNYEFFLPDNMEALQIRKQCALAALKDVHNYLSHEEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICNDPGIIAENIRQVKLGSPDYIDCDREKVLEDFLKRIECYEVNYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHIQSRTVYYLMNIHVTPRSIYLCRHGESELNIRGRIGGDSGLSVRGKQYAYALANFIQSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCKVESIYLNVEAVNTHREKPENVDITREPEEALDTVPAHY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Rabbit

100

Xenopus

Zebrafish

Pig

Dog

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P16118 As Substrate

P16118

Site	PTM Type	Source
S2	Phosphorylation	Uniprot
S24	Phosphorylation	Uniprot
T39	Phosphorylation	Uniprot
T133	Phosphorylation	Uniprot
Y200	Phosphorylation	Uniprot
Y204	Phosphorylation	Uniprot
Y227	Phosphorylation	Uniprot
Y242	Phosphorylation	Uniprot
Y243	Phosphorylation	Uniprot
S262	Phosphorylation	Uniprot
K364	Ubiquitination	Uniprot
Y438	Phosphorylation	Uniprot

Research Backgrounds

P16118_F261_HUMAN

Function:

Synthesis and degradation of fructose 2,6-bisphosphate.

Tissue Specificity:

Liver.

Subunit Structure:

Homodimer.

Family&Domains:

In the C-terminal section; belongs to the phosphoglycerate mutase family.

Research Fields

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

PFKFB1 Antibody - #DF3098