Product: FRA1 Antibody
Catalog: DF3096
Description: Rabbit polyclonal antibody to FRA1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Sheep
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 43 KD; 29kD(Calculated).
Uniprot: P15407
RRID: AB_2835396

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Sheep
Prediction:
Pig(91%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
FRA1 Antibody detects endogenous levels of total FRA1.
RRID:
AB_2835396
Cite Format: Affinity Biosciences Cat# DF3096, RRID:AB_2835396.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FOS L1; FOS like antigen 1; Fos related antigen 1; Fos-related antigen 1; FOSL 1; FOSL1; FOSL1 protein; FOSL1_HUMAN; FRA 1; FRA-1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MFRDFGEPGPSSGNGGGYGGPAQPPAAAQAAQQKFHLVPSINTMSGSQELQWMVQPHFLGPSSYPRPLTYPQYSPPQPRPGVIRALGPPPGVRRRPCEQISPEEEERRRVRRERNKLAAAKCRNRRKELTDFLQAETDKLEDEKSGLQREIEELQKQKERLELVLEAHRPICKIPEGAKEGDTGSTSGTSSPPAPCRPVPCISLSPGPVLEPEALHTPTLMTTPSLTPFTPSLVFTYPSTPEPCASAHRKSSSSSGDPSSDPLGSPTLLAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
91
Xenopus
82
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P15407 As Substrate

Site PTM Type Enzyme
R66 Methylation
T69 Phosphorylation
S74 Phosphorylation
R79 Methylation
R84 Methylation
S101 Phosphorylation
K144 Ubiquitination
T183 Phosphorylation
S191 Phosphorylation
T217 Phosphorylation
T223 Phosphorylation Q04759 (PRKCQ)
T227 Phosphorylation
T230 Phosphorylation Q04759 (PRKCQ)
T240 Phosphorylation
S246 Phosphorylation
S251 Phosphorylation
S252 Phosphorylation
S253 Phosphorylation
S254 Phosphorylation
S265 Phosphorylation Q04759 (PRKCQ)
T267 Phosphorylation

Research Backgrounds

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterodimer.

Family&Domains:

Belongs to the bZIP family. Fos subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

References

1). ETV4 is a theranostic target in clear cell renal cell carcinoma that promotes metastasis by activating the pro-metastatic gene FOSL1 in a PI3K-AKT dependent manner. CANCER LETTERS, 2020 (PubMed: 32305558) [IF=9.7]

Application: WB    Species: human    Sample: ccRCC cell

Fig. 5. The correlation of ETV4 and FOSL1 in cell lines and other tumor type tissues. (A). The clinical distribution of ETV4 and FOSL1 were analyzed by using the TCGA ccRCC data set. (B). The protein level were determined by western blotting showing that ETV4 and FOSL1 were correlatively expressed in ccRCC cell lines. (C), (E) and (G). Transient suppression of ETV4 in ACHN, ETV4 and FOSL1 mRNA level was detected by qPCR. Data represent the mean ± S.D. ***p < 0.0001. ETV4 and FOSL1 expression were measured by immunoblotting. (D), (F) and (H). Transient suppression of ETV4 in SN12C, ETV4 and FOSL1 mRNA level was detected by qPCR. Data represent the mean ± S.D. ***p < 0.0001. ETV4 and FOSL1 expression were measured by immunoblotting. (I) Localization of Vimentin in ACHN NC, ACHN si# 1 cells by confocal immunofluorescence analysis. (J). Suppression of FOSL1 dramatically reduced the migration ability of the cells as determined by a wound-healing assay. Yellow dashed lines denote the margins of the wound. (K). The quantified data of wound width of transwell. Columns, average of three independent experiments; bars, SD. *p < 0.05, Student t-test. (L) and (M). The migratory ability of overexpressed ETV4 and suppressed FOSL1 in ACHN cells. Data represent the mean ± S.D. *p < 0.05. . (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

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