CDKA1 / DOC1 Antibody | Affinity Biosciences

Product:	CDKA1 / DOC1 Antibody
Catalog:	AF0309
Description:	Rabbit polyclonal antibody to CDKA1 / DOC1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Mol.Wt.:	20kDa; 12kD(Calculated).
Uniprot:	O14519
RRID:	AB_2833473

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

CDKA1 / DOC1 Antibody detects endogenous levels of total CDKA1 / DOC1.

RRID:

AB_2833473
Cite Format: Affinity Biosciences Cat# AF0309, RRID:AB_2833473.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CDK2 A1; CDK2 associated protein 1; CDK2-associated protein 1; CDK2AP1; CDKA1; CDKA1_HUMAN; Cyclin dependent kinase 2 associated protein 1; Cyclin-dependent kinase 2-associated protein 1; Deleted in oral cancer 1; DOC 1; DOC 1 related protein; DOC 1R; DOC-1; DOC1; DOC1R; DORC1; p12DOC 1; Putative oral cancer suppressor; ST19;

Immunogens

Immunogen:

A synthesized peptide derived from human CDKA1 / DOC1, corresponding to a region within C-terminal amino acids.

Uniprot:

O14519(CDKA1_HUMAN) >>Visit HPA database.

Gene(ID):

CDK2AP1(8099)

Description:

CDK2AP1 Belongs to the CDK2AP family.

Sequence:

O14519 CDKA1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSYKPNLAAHMPAAALNAAGSVHSPSTSMATSSQYRQLLSDYGPPSLGYTQGTGNSQVPQSKYAELLAIIEELGKEIRPTYAGSKSAMERLKRGIIHARGLVRECLAETERNARS

PTMs - O14519 As Substrate

O14519

Site	PTM Type	Enzyme	Source
S24	Phosphorylation		Uniprot
S46	Phosphorylation	Q14164 (IKBKE)	Uniprot
S84	Phosphorylation		Uniprot
K85	Ubiquitination		Uniprot
S86	Phosphorylation		Uniprot

Research Backgrounds

O14519_CDKA1_HUMAN

Function:

specific inhibitor of the cell-cycle kinase CDK2.

PTMs:

Phosphorylated in vitro by IKBKE at Ser-46.

Subunit Structure:

Homodimer. Interacts with monomeric unphosphorylated CDK2 (By similarity). Interacts with CDK2AP2.

Family&Domains:

Belongs to the CDK2AP family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

CDKA1 / DOC1 Antibody - #AF0309